164,577 results

1. LV-miR-142

   Plasmid

   #117056

   Purpose

   Overexpression of miR-142-3p using miR-451 backbone to enable DICER-independent expression along with orange fluorescent protein (OFP) to track miRNA expression

   Depositor

   Michele De Palma

   Article

   Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

   Insert

   miR-142-3p - OFP (Mir142 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV promoter

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACEBac1-GCP2

   Plasmid

   #178068

   Purpose

   Insect cell expression of GCP2

   Depositor

   Tarun Kapoor

   Article

   Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

   Insert

   GCP2 (TUBGCP2 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRE-Tn5-163

   Plasmid

   #118542

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sYFP2
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. R620-M12-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.351

   Plasmid

   #170196

   Purpose

   Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
   

   Use

   Tags

   3C-His8-Strep2x2

   Expression

   Mammalian

   Mutation

   L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…

   Promoter

   CMV51

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CDC40

   Plasmid

   #155444

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   CDC40 (CDC40 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. LCV2 V-1

   Plasmid

   #84804

   Purpose

   Cas9 with sgRNA targeting Exon 1 of VEGF-A

   Depositor

   Glenn Yiu

   Article

   Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

   Insert

   gRNA targeting VEGF-A (VEGFA Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. XX01CAPN2A-c001

   Plasmid

   #175492

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-L700 (CAPN2); EF hand 1-5, H86-S268 (CAPNS1). CAPN2 can be biotinylated. Codon optimised.

   Depositor

   Opher Gileadi

   Insert

   CAPN2 / CAPNS1 (CAPN2 Human)
   

   Use

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRE-Tn5-164

   Plasmid

   #118544

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mOrange2
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRE-Tn5-166

   Plasmid

   #118546

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mCardinal
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCF525-EF1a-Hygro-P2A-mCherry-lenti

    Plasmid

    #115796

    Purpose

    Expresses mCherry in human cells, contained in a lentiviral vector.

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF-1a promoter

    Available Since

    Oct. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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