166,289 results

1. WT Smarca4-V5 IRES-puro

   Plasmid

   #107058

   Purpose

   Expression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.

   Depositor

   Jerry Crabtree, Courtney Hodges

   Article

   Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

   Insert

   SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
   

   Use

   Lentiviral

   Tags

   IRES-puroR and V5

   Expression

   Mammalian

   Mutation

   wild-type

   Promoter

   CAG

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. LRG2.1-TagBFP2

   Plasmid

   #124773

   Purpose

   Lentiviral plasmid to co-express a guide RNA and TagBFP2

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPFO*

   Plasmid

   #120400

   Purpose

   Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.

   Depositor

   Arun Radhakrishnan

   Article

   Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11

   Insert

   Mutant form of Perfringolysin O
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Amino acids 1-27 are deleted, starts at amino aci…

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLF3FShC

   Plasmid

   #153083

   Purpose

   RMCE integration vector with self excising cassette

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Type

   Empty backbone

   Use

   Recombination mediated cassette exchange

   Expression

   Worm

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223-MERTK

   Plasmid

   #23900

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   MERTK (MERTK Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX333-T2A-EGFP

   Plasmid

   #197417

   Purpose

   SpCas9-T2A-EGFP with dual sgRNA cloning backbones

   Depositor

   Yeh-Hsing Lao

   Article

   Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CBh; U6

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cbh_v5 AAV-ABE N-terminal

   Plasmid

   #137177

   Purpose

   AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   v5 AAV-ABE N-terminal
   

   Use

   AAV

   Mutation

   Cas9 D10A

   Promoter

   Cbh

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpdCas9 KRAB

   Plasmid

   #188499

   Purpose

   Expresses FLAG tagged SpdCas9 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   EF1a

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO_CalR-SNAP-tag2-KDEL

   Plasmid

   #226517

   Purpose

   Mamalian cell expression of SNAP-tag2 in endoplasmic reticulum

   Depositor

   Kai Johnsson

   Article

   Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

   Insert

   CalR-SNAP-tag2-KDEL
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223_GOPC_WT

    Plasmid

    #82924

    Purpose

    Gateway Donor vector containing GOPC, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    GOPC (GOPC Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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