We narrowed to 9,182 results for: Pol

1. pPLP2S-mCf

   Plasmid

   #229986

   Purpose

   LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. Strep-tag II CP tag for Strep-Tactin AC purification.

   Depositor

   Barry Lutz

   Article

   Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39

   Type

   Empty backbone

   Tags

   Strep-tag II

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. p5M-PR-A

   Plasmid

   #89124

   Purpose

   mammalian expression of full-length human progesterone receptor-A in pCMV5 with 3 restriction sites deleted in polylinker

   Depositor

   Elizabeth Wilson

   Article

   Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

   Insert

   human progesterone receptor-A (PGR Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Col2.3deltaTK

   Plasmid

   #114273

   Purpose

   Conditional ablation of mature bone cells via ganciclovir exposure of cells expressing a truncated thymidine kinase.

   Depositor

   David Rowe

   Article

   Visnjic et al J Bone Miner Res. 2001 Dec;16(12):2222-3

   Insert

   Col2.3 promoter + deltaTK + BGH polyA
   

   Expression

   Mammalian

   Promoter

   2.3 kb rat type 1 collagen

   Available Since

   Oct. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. DCAF1:PBC020-D05:C246337

   Plasmid

   #183974

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   DCAF1:PBC020-D05:C246337 (DCAF1 Human)
   

   Use

   Baculovirus expresssion

   Tags

   6xHis-TEV and MHHHHHHSSGRENLYFQG

   Promoter

   Polyhedrin

   Available Since

   May 10, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. MF87_PB-EF1a-TET3G-P2A-ERT2-Gal4-BGHpA

   Plasmid

   #176630

   Purpose

   Integrated in CHO-K1 to make parental polyclonal lines for all other MultiFate cell lines

   Depositor

   Michael Elowitz

   Article

   Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

   Insert

   EF1a-TET3G-P2A-ERT2-Gal4-BGHpA
   

   Use

   Synthetic Biology

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-mCherry-CAAX

   Plasmid

   #166229

   Purpose

   Lentiviral plasmid for expression of mCherry with a C-terminal CAAX polybasic sequence from KRras targeting mCherry to the plasma membrane.

   Depositor

   Carlos Carmona-Fontaine

   Article

   Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

   Insert

   mCherry-CAAX
   

   Use

   Lentiviral

   Tags

   CAAX

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. MTK4_002

   Plasmid

   #123811

   Purpose

   Encodes SV40 polyadenylation signal as a Type 4 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   SV40 pA
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT3TS_Transposase

   Plasmid

   #109768

   Purpose

   Codon optimised transposase sequence in an optimal vector for in vitro transcription. Contains the T3 promoter and synthetic polyA tract.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Tol2 Transposase
   

   Use

   Zebrafish plasmids

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2_Renilla_luc-60A

   Plasmid

   #200397

   Purpose

   A plasmid IVT template containing a Renilla luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

   Depositor

   Xiao Wang

   Article

   Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

   Insert

   Renilla luciferase
   

   Expression

   Mammalian

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFS462

    Plasmid

    #89065

    Purpose

    his7 integration plasmid with Z3EVpr:GFP

    Depositor

    Nick Rhind

    Article

    Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

    Insert

    Z3EV promoter GFP-NLS leu1 C-terminal 300 nt
    

    Use

    S. pombe his7 integration vector

    Promoter

    Z3EV

    Available Since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CMV-dSa-VPR mini.-2X snRP1

    Plasmid

    #99688

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signal

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Mutation

    dead Cas9

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2,0_3E5E_eGFP

    Plasmid

    #69359

    Purpose

    Encodes for Ebola Minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Schmidt et al J Infect Dis. 2011 Nov;204 Suppl 3:S861-

    Insert

    3E5E eGFP minigenome
    

    Use

    T7

    Promoter

    T7

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+) prME (JEV) sdm2

    Plasmid

    #52881

    Purpose

    expression of Japanese Encephalitis Virus VLPs in mammalian cells

    Depositor

    Yorgo Modis

    Article

    Nour et al PLoS Pathog. 2013;9(9):e1003585. doi: 10

    Insert

    prME (POLY Japanese encephalitis virus)
    

    Expression

    Mammalian

    Mutation

    sdm2

    Promoter

    CMV

    Available Since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pSmart-EF1a-Nla-cas11

    Plasmid

    #178882

    Purpose

    Expression of human codon-optimized NlaCas11 with N terminal NLS and HA tag

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas11c-bGH polyA
    

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJEP205-AAV-TRE3G-GFP-pA

    Plasmid

    #164789

    Purpose

    AAV vector backbone designed to express GFP from a TRE3G promoter. It also contains a poly-Adenylation Signal (pA)

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Green Fluorescent protein (GFP)
    

    Use

    AAV

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. RNF168-Halo HRD

    Plasmid

    #207080

    Purpose

    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
    

    Expression

    Mammalian

    Promoter

    None

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRN3P_T3_ABEmax_IVT

    Plasmid

    #171761

    Purpose

    Plasmid to be used as DNA template for in-vitro RNA transcription of the ABEmax base editor (A to G) by T3 RNA polymerase

    Depositor

    Timo Otonkoski

    Article

    Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

    Insert

    ABEmax
    

    Use

    Vector for in-vitro transcription

    Promoter

    T3 promoter

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDctp-mSox10-Mp1

    Plasmid

    #13513

    Depositor

    William Pavan

    Article

    Hakami et al Mech Dev. 2006 Feb . 123(2):124-34.

    Insert

    Sox10 (Sox10 Mouse)
    

    Tags

    Dct promoter and Mp1-polyA

    Expression

    Mammalian

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTK312

    Plasmid

    #59569

    Purpose

    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP XbaI attB1 Kozak EGFP ochre attB2 AscI
    

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual

    Plasmid

    #216377

    Purpose

    Baculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)

    Depositor

    William Weis

    Insert

    LDL receptor related protein 6 (LRP6 Human)
    

    Use

    Baculovirus

    Tags

    9xHis and HA signal sequence-FLAG-3C cleavage sit…

    Expression

    Insect

    Promoter

    Polyhedrin (LRP6)/p10 (MESD)

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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