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pcDNA5-FRT-TRE3GS-eGFP

Plasmid

#252871

Purpose

TRE3GS expression plasmid for Flp/FRT recombination expressing eGFP

Depositor

Norman Davey

Insert

eGFP

Expression

Mammalian

Promoter

TRE3GS

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-EBFP-VAV1-W495L

Plasmid

#242839

Purpose

Encodes human VAV1 and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

VAV1 (VAV1 Human)

Use

Lentiviral

Tags

EBFP2

Expression

Mammalian

Mutation

W495L

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-EBFP2-VAV1-E201A

Plasmid

#242838

Purpose

Encodes human VAV1 and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

VAV1 (VAV1 Human)

Use

Lentiviral

Tags

EBFP2

Expression

Mammalian

Mutation

E201A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-WASP-3D

Plasmid

#242836

Purpose

Encodes human WASP and N-terminal EGFP tag

Depositor

Cheng-han Yu

Article

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

WASP (WAS Human)

Tags

EGFP

Expression

Mammalian

Mutation

F244D, H246D, H249D

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pYO734

Plasmid

#235753

Purpose

Protein expression of ScVPS30 and ZZ-ScVPS38

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS30 (VPS30 Budding Yeast)
VPS38 (VPS38 Budding Yeast)

Tags

ZZ-3xTEV

Expression

Yeast

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1101

Plasmid

#235708

Purpose

Expression of FL human Beclin 1 and FL human ATG14L in mammalian cells

Depositor

Roger Williams

Article

Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

Inserts

Beclin 1 (BECN1 Human)
ATG14L (ATG14 Human)

Expression

Mammalian

Promoter

CAG and CMV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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GST-RNF126 C13,16A

Plasmid

#138645

Purpose

Bacterial expression of GST-RNF126 ZnF mutant (C13,16A) fusion protein

Depositor

Jane McGlade

Article

Smith et al J Cell Sci. 2013 Mar 15;126(Pt 6):1366-8

Insert

RNF126 (RNF126 Mouse)

Tags

GST

Expression

Bacterial

Mutation

C13A, C16A

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-TagRFP-T-EEA1

Plasmid

#254776

Purpose

Neutrophil specific Early Endosome Antigen 1 expression in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

EEA1 (EEA1 Human)

Use

Zebrafish expression

Tags

TagRFP-T

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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AAVS1 locus donor plasmid

Plasmid

#252870

Purpose

Plasmid encoding FRT recombination site and TetOn3G transactivator flanked by AAVS1 homology arms

Depositor

Norman Davey

Insert

TetOn3G transactivator and FRT recombination site

Expression

Mammalian

Available Since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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pYO979

Plasmid

#235756

Purpose

Protein expression of ScVPS38, untagged + ScVPS30 FRK to DDD at 430-432, untagged

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS38 (VPS38 Budding Yeast)
VPS30 (VPS30 Budding Yeast)

Expression

Yeast

Mutation

FRK430-432DDD

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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pYO539

Plasmid

#235752

Purpose

Protein expression of ScVPS30 and ScVPS38

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS30 (VPS30 Budding Yeast)
VPS38 (VPS38 Budding Yeast)

Expression

Yeast

Promoter

GAL-TDH3

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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MAC_C_CSF1R

Plasmid

#187767

Purpose

MAC-tagged gene expression of human CSF1R

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

CSF1R (CSF1R Human)

Expression

Mammalian

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pGST2-yVps23-UEV_C133A

Plasmid

#45924

Depositor

James Hurley

Article

Ren et al EMBO J. 2011 Jun 1;30(11):2130-9. doi: 1

Insert

yeast Vps23-UEV(1-160) (STP22 Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

C133A

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
BPK1520-sgRNA GLB1

Plasmid

#184378

Purpose

Expresses a sgRNA to edit GLB1 gene NM_000404.2_c.907A>G

Depositor

David Gilot

Article

Leclerc et al CRISPR J. 2023 Feb;6(1):17-31. doi: 10.1

Insert

sgRNA targeting GLB1 (GLB1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pBoBi-hSEC61B-C-PEN2

Plasmid

#183648

Purpose

Lentiviral expression of a ER-residential PEN2.

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 and SEC61B (PSENEN, SEC61B Human)

Use

Lentiviral

Tags

HA

Mutation

WT

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBoBi-hPEN2 2A

Plasmid

#183640

Purpose

Lentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the N-terminus (cytosolic face).

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 (PSENEN Human)

Use

Lentiviral

Tags

HA

Mutation

residues 35 and 40 of PEN2 mutated to alanine

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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Negative feedback (NF) plasmid

Plasmid

#169747

Purpose

Standalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

tetR-P2A-eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV-tetO2

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt2(K14A/R25E)-Halo-PS

Plasmid

#86632

Purpose

Expresses eGFP-Akt2-HaloTag-PS fusion (cytosolic tandem)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2(K14A/R25E)-Halo-PS (AKT2 )

Expression

Mammalian

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pOGGFPR

Plasmid

#64398

Purpose

Oat globulin Glo1 promoter driving expression of GFP

Depositor

Claudia Vickers

Article

Vickers et al Plant Mol Biol. 2006 Sep;62(1-2):195-214

Insert

Oat globulin Glo1 promoter

Tags

GFP

Expression

Plant

Promoter

Oat globulin Glo1

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pT.cruzi-polyubiquitin_6xHis

Plasmid

#256080

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Article

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

Polyubiquitin

Tags

6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available Since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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