We narrowed to 9,182 results for: Pol

1. pX330-NEAT1m_v1

   Plasmid

   #97083

   Purpose

   Encodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoform

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   sgRNA NEAT1m_v1
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOCC7

   Plasmid

   #118876

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRN3P_T3_BE4_IVT

   Plasmid

   #171762

   Purpose

   Plasmid to be used as DNA template for in-vitro RNA transcription of the BE4 base editor (C to T) by T3 RNA polymerase

   Depositor

   Timo Otonkoski

   Article

   Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

   Insert

   BE4
   

   Use

   Vector for in-vitro transcription

   Promoter

   T3 promoter

   Available Since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOCC154

   Plasmid

   #118902

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
   

   Tags

   TwinStrep, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-V96 ELP

   Plasmid

   #67853

   Purpose

   GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

   Depositor

   Andrew MacKay

   Article

   Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

   Insert

   GFP fused to ELP (VPGVG) repeated 96 times
   

   Tags

   GFP fused to N-terminal of ELP

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX335-NEAT1_IS_v1

   Plasmid

   #97085

   Purpose

   Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   sgRNA NEAT1_IS_v1
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOCC153

   Plasmid

   #118901

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   TwinStrep, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX335-NEAT1_IS_v2

   Plasmid

   #97086

   Purpose

   Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   sgRNA NEAT1_IS_v2
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-PCBP1

   Plasmid

   #16177

   Depositor

   Huda Zoghbi

   Article

   Lim et al Cell. 2006 May 19. 125(4):801-14.

   Insert

   Poly(rC) binding protein 1 (PCBP1 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

10. Clathrin-LCa-ECFP

    Plasmid

    #21742

    Depositor

    Xiaowei Zhuang

    Article

    Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Tags

    ECFP

    Expression

    Mammalian

    Available Since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

11. hPDI1-18-508_C400S_C56S-pFLAG-CMV1

    Plasmid

    #31383

    Depositor

    David Ron

    Article

    Zito et al Mol Cell. 2010 Dec 10. 40(5):787-97.

    Insert

    PDI1A (P4HB Human)
    

    Tags

    FLAG M2

    Expression

    Mammalian

    Mutation

    C-terminal cysteines of both active sites mutated…

    Available Since

    May 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. MX01

    Plasmid

    #158624

    Purpose

    GATEWAY and BacMam compatible mammalian expression vector with eGFP expression reporter (eGFP is expressed by same promotor but as a separate polypeptide chain).

    Depositor

    Timm Maier

    Article

    Scaiola et al Sci Adv. 2020 Nov 6;6(45). pii: 6/45/eab

    Type

    Empty backbone

    Use

    Gateway destination vector ; bacmam acceptor vect…

    Tags

    P2A

    Expression

    Mammalian

    Promoter

    CMV-IE

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-T3-hCasD10A-pA

    Plasmid

    #51638

    Purpose

    Expresses Cas9-nickase in mammalian cells and zygotes.

    Depositor

    Wataru Fujii

    Article

    Fujii et al Biochem Biophys Res Commun. 2014 Jan 31.

    Insert

    Codon-optimized Cas9 D10A
    

    Use

    CRISPR

    Tags

    FLAG, NLS, and polyA

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pCas1_Cas2/3

    Plasmid

    #89240

    Purpose

    Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1

    Depositor

    Blake Wiedenheft

    Article

    Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

    Insert

    Cas1 and Cas2/3
    

    Tags

    Strep II tag on Cas1

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. SP6-hCas9-Ce-mRNA

    Plasmid

    #47911

    Purpose

    In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.

    Depositor

    Paul Sternberg

    Article

    Chiu et al Genetics. 2013 Aug 26.

    Insert

    hCas9 with C. elegans 5' and 3' UTRs
    

    Use

    CRISPR

    Promoter

    SP6

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. TAF1

    Plasmid

    #39118

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TAF1 (TAF1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pBEAST_J23101-amidase

    Plasmid

    #128135

    Purpose

    The cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    amidase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23101

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pL2-GGA(EAAAK)2AGG

    Plasmid

    #137104

    Purpose

    iFLinkC Linker cloning

    Depositor

    Viktor Stein

    Article

    Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

    Insert

    GGA(EAAAK)2AGG
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT3TS-nCas9n-A71

    Plasmid

    #125644

    Purpose

    micro-injection of poly-adenylated nCas9n RNA

    Depositor

    Jason Berman

    Article

    Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

    Insert

    nCas9n
    

    Use

    Rna in vitro transcription vector

    Promoter

    T3

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3922-48

    Plasmid

    #176497

    Purpose

    pTRE-EGFP-T2A-TFEB(wt)-polyA

    Depositor

    Albert La Spada

    Article

    Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

    Insert

    EGFP-T2A-TFEB(wild-type; full-length; human)
    

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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