Addgene

We narrowed to 21,567 results for: ESC;

Showing: 8141 - 8160 of 21567 results

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Ub-LPNTG-pET

Plasmid

#221850

Purpose

Expresses Ub with sortase motif and His tag

Depositor

Artur Krężel

Article

Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

Insert

Ubiquitin

Use

Tags

Expression

Bacterial

Mutation

Arg74ProLeuThr

Promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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GB4650

Plasmid

#215265

Purpose

TU for the copper regulated expression of the PhiC31 recombinase.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS:miniDFR:PhiC31:tNos

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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GB4652

Plasmid

#215266

Purpose

TU for the copper regulated expression of the flippase (FLP) recombinase.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS:miniDFR:FLP:TNOS

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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GB1203

Plasmid

#215369

Purpose

TU for the expression of the silencing suppressor P19 driven by the 35s promoter

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

35s:P19:tNos

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pWR95(URA3)

Plasmid

#203917

Purpose

BLINCAR plasmid for gene deletion, URA3 target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

URA3 upstream homology
URA3 downstream homology

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-BBS18

Plasmid

#218718

Purpose

Expresses N-terminally EGFP-tagged BBS18 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS18

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-SFFVp-gI-BlastR

Plasmid

#207179

Purpose

Gateway compatible Lentiviral Expression Plasmid SFFVp-IRES-BlastR

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFVp

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB605-GBSSI

Plasmid

#213867

Purpose

Binary vector for the expression of GBBSI fused to the sGFP in plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ichikawa et al Plant Physiol. 2023 Oct 4:kiad528. doi:

Insert

GRANULE-BOUND STARCH SYNTHASE I (GBSSI) (GBSS1 Mustard Weed)

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB560-GBSSI

Plasmid

#213866

Purpose

Binary vector for the expression of GBBSI fused to the tagRFP in plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ichikawa et al Plant Physiol. 2023 Oct 4:kiad528. doi:

Insert

GRANULE-BOUND STARCH SYNTHASE I (GBSSI) (GBSS1 Mustard Weed)

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBbA5k-AcrAB-AsLOV2*(543)

Plasmid

#210863

Purpose

IPTG inducible promoter (lacUV5) expressing AcrA and AcrB-AsLOV2*(543). AsLOV2*(543) is also described in the literature as LOVdeg.

Depositor

Mary Dunlop

Article

Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

Inserts

acrA
acrB

Use

Synthetic Biology

Tags

AsLOV2*(543)

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PRLSS-yATOM_SH2-KDEL

Plasmid

#209714

Purpose

Mammalian expression of y-ATOM biosensor for SH2 localized to endoplasmic reticulum

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

PRLSS-yATOM_SH2-KDEL

Use

Tags

PRLSS

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_WDR5 (R80A)

Plasmid

#209713

Purpose

Mammalian expression of y-ATOM biosensor for WDR5 with binding null R80A mutation

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_WDR5 (R80A)

Use

Tags

MBP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PRLSS-NbGFP_miRFP670nano3-KDEL

Plasmid

#209718

Purpose

Mammalian expression of NbGFP-miRFP670nano3 localized to endoplasmic reticulum

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

PRLSS-NbGFP_miRFP670nano3-KDEL

Use

Tags

PRLSS

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PRLSS-Clover-KDEL

Plasmid

#209720

Purpose

Mammalian expression of clover localized to endoplasmic reticulum

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

PRLSS-Clover-KDEL

Use

Tags

PRLSS

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Cox4-yATOM_SH2

Plasmid

#209715

Purpose

Mammalian expression of y-ATOM biosensor for SH2 localized to mitochondria

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

Cox4-yATOM_SH2

Use

Tags

Cos4

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pWR95

Plasmid

#203916

Purpose

BLINCAR plasmid for gene deletion, no genomic target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

modified MCS

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-SNIFP

Plasmid

#197210

Purpose

Expresses the protein of SNIFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

SNIFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSF11-wmCardinal-T2A-HO1

Plasmid

#197249

Purpose

Expresses the protein of wmCardinal-T2A-HO1 in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmCardinal-T2A-HO1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSF11-wmiRFP720-T2A-HO1

Plasmid

#197248

Purpose

Expresses the protein of wmiRFP720-T2A-HO1 in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmiRFP720-T2A-HO1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-pnRFP

Plasmid

#201678

Purpose

Bacteria protein expression of pnRFP biosensor

Depositor

Huiwang Ai

Article

Pang et al ACS Chem Biol. 2023 Jun 16;18(6):1388-13

Insert

pnRFP

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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