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pBAD33(A-N)

Plasmid

#187084

Purpose

Medium expression of Complex I with subunit N-HA tagged

Depositor

Steven Vik

Article

Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

Insert

nuoA-N

Use

Tags

HA tag nuoN

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD33(EFG)

Plasmid

#187087

Purpose

Medium expression of subunits EFG

Depositor

Steven Vik

Article

Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

Insert

nuoEFG

Use

Tags

HA tag nuoG

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pTETDuet_WTDHFR_WTTS

Plasmid

#191282

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

WT DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAW209

Plasmid

#113188

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAB172a

Plasmid

#156324

Purpose

Expresses heterologous rRNA

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

rRNA operon, orthogonal-antiRBS

Use

Synthetic Biology

Tags

Expression

Mutation

WT antiRBS engineered to orthogonal antiRBS

Promoter

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSPK1

Plasmid

#18876

Depositor

Jay Keasling

Article

Van Dien et al Appl Environ Microbiol. 1997 May . 63(5)

Insert

polyphosphate kinase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2008

Availability

Academic Institutions and Nonprofits only
PAS513 (TJF080)

Plasmid

#65580

Purpose

pETDuet-1 Containing FadD S379R

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Use

Tags

Expression

Bacterial

Mutation

S379R

Promoter

T7 lacO

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
PAS529 (TJF072)

Plasmid

#65603

Purpose

pETDuet-1 Containing FadD Q338R-His

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Use

Tags

6x His

Expression

Bacterial

Mutation

Q338R

Promoter

T7 lacO

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
PAS504 (TJF067)

Plasmid

#65571

Purpose

pETDuet-1 Containing FadD F447S

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD with F447S mutation

Use

Tags

Expression

Bacterial

Mutation

F447S

Promoter

T7 lacO

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
pGU06

Plasmid

#212148

Purpose

Plasmid expressing alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase and tyrosine aminotransferase under constitutive promoter PgyrA control.

Depositor

Kang Zhou

Insert

alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase, tyrosine aminotransferase (AGX1, alaA, tyrB Budding Yeast, Agx1: S. cerevisiae; alaA, tyrB: E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

PgyrA

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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