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pET-51b(+)-cpHalo∆

Plasmid

#194659

Purpose

Protein production of the split HaloTag part cpHalo∆ (cpHaloTag135/156) in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

cpHalo∆

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pEF HaloTag-SYT1

Plasmid

#197280

Purpose

Encodes full-length wild type synaptotagmin 1 (SYT1) with an N-terminal HaloTag

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYT1 and HaloTag (Syt1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(cyto).cpSFGFP.HaloTag

Plasmid

#209658

Purpose

Non-responsive control

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.HaloTag

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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N-terminal Halo-XLF

Plasmid

#207546

Purpose

Homologous recombination donor to insert halo tag at the N terminal of the endogenous XLF locus.

Depositor

Jens Schmidt

Article

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

HaloTag with internal PuroR cassette flanked by human Lig4 locus sequences

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHTC-HaloTag_FBXO3:1-471

Plasmid

#211072

Purpose

Mammalian expression of full-length human FBXO3. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXO3:M1-F471 (FBXO3 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSEMS-CV SUy-Halo7Tag

Plasmid

#108933

Purpose

Selflabeling tag at ATP Synthase

Depositor

Karin Busch

Article

Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

Insert

ATP synthase F1 subunit gamma (ATP5F1C Human)

Use

Tags

Halo7 tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pB-Halo,IRES-eGFP

Plasmid

#164519

Purpose

All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible GFP and constitutive rtTA and neomycin resistance)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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FCK-Halo-GFP

Plasmid

#14750

Purpose

Lentiviral expression of Halo-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al PLoS One. 2007 Mar 21;2(3):e299.

Insert

Mammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

N. pharaonis halorhodopsin is mammalian codon-opt…

Promoter

CaMKIIa

Available since

April 5, 2007

Availability

Academic Institutions and Nonprofits only

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SSpB-HaloTag-p63DH

Plasmid

#176116

Purpose

Heterodimerization with iLID, RhoA activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

RHOGEF p63 (ARHGEF25 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-No-HaloTag7-LC3

Plasmid

#184900

Purpose

Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Use

Tags

HaloTag7

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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