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  3. pBRα-σ70 D581G

    Plasmid
    #53732
    Purpose
    Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The sigma 70 moiety carries the D581G substitution
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pSS9

    Plasmid
    #71655
    Purpose
    Contains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coli
    Depositor
    Ryan Gill
    Article
    Bassalo et al ACS Synth Biol. 2016 Apr 22.
    Insert
    gfpuv
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    T7A1
    Available since
    Jan. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    71655-plasmid-map-sequence-id-257561

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Showing: 401 - 410 of 638 results