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pLEX_305-N-dTAG

Plasmid

#91797

Purpose

Lentiviral/gateway cloning vector for N-terminally tagging proteins of interest with the dTAG system

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pX330A-nBRD4/PITCh

Plasmid

#91794

Purpose

PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

SpCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

chicken β-actin promoter

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/MPL-HA

Plasmid

#204530

Purpose

Mammalian expression of human MPL-HA

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

MPL-HA (MPL Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET30a-HS-Tn5

Plasmid

#127916

Purpose

Prokaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.

Depositor

Silin Zhong

Article

Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

Insert

Hyperstable Tn5

Use

Tags

E coli elongation factor Ts and Mxe intein - Chit…

Expression

Bacterial

Mutation

E54K, L372P

Promoter

T7

Available since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA1-Tat

Plasmid

#138478

Purpose

Expresses HIV Tat for efficient sgRNA packaging.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

Tat

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST/hCx43-EGFP-N1

Plasmid

#40907

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

Connexin 43 (GJA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

No stop codon to create c-terminal fusion

Promoter

CMV

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

Plasmid

#181745

Purpose

dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

Depositor

Benjamin Kleinstiver

Article

Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

Insert

human/zebrafish codon optimized SpCas9

Use

In vitro transcription; t7 promoter

Tags

NLS(SV40)-3xFLAG

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAJ075_hsCRBN

Plasmid

#124214

Purpose

Insect cell expression vector for His6 tagged hsCRBN

Depositor

Eric Fischer

Article

Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

Insert

Cereblon (CRBN Human)

Use

Tags

His6-TEV

Expression

Insect

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-PD-1 (Y223F, Y248F)-mGFP

Plasmid

#180790

Purpose

Expression of human PD-1 (Y223F, Y248F) fused to mEGFP

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

PD-1 (Y223F, Y248F) (PDCD1 Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mutation

PD-1 (Y223F, Y248F)

Promoter

SFFV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mClover3-KASH5-DN

Plasmid

#171492

Purpose

T7 promotor drives in vitro transcription of mClover3-tagged bovine Kash5 DN mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

KASH5-DN (CCDC155 B. tarus (bovine))

Use

Pgehme

Tags

mClover3

Expression

Mutation

Promoter

T7

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMHE-GST-KASH5-DN

Plasmid

#171493

Purpose

T7 promotor drives in vitro transcription of GST-tagged bovine Kash5 DN mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

KASH5-DN (CCDC155 B. tarus (bovine))

Use

Pgehme

Tags

GST

Expression

Mutation

Promoter

T7

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_3xFLAG_V5_ eGFP_V5

Plasmid

#160111

Purpose

Expresses eGFP in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

eGFP

Use

Lentiviral

Tags

3xFLAG, V5

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR wt-FLAG

Plasmid

#204532

Purpose

Mammalian expression of human CALR wt-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR wt-FLAG (CALR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-CD28

Plasmid

#223596

Purpose

Lentiviral expression of human CD28 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PAX5_PAX5

Plasmid

#205887

Purpose

Express mEGFP-tagged fusion protein, PAX5_PAX5 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PAX5_PAX5 (PAX5 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/MPL-V5

Plasmid

#204531

Purpose

Mammalian expression of human MPL-V5

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

MPL-V5 (MPL Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry)

Plasmid

#216531

Purpose

Targeting vector for CRISPRd generation

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

ABI-dCas9-mCherry

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His

Plasmid

#171587

Purpose

Bacterial expression of NLS-RfxCas13d-NLS 6His

Depositor

Neville Sanjana

Article

Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

Insert

MKIEE-NLS-RfxCas13d-NLS-HA-6His

Use

Tags

HA-6HIS and MKIEE

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pQCX-CD28 WT

Plasmid

#223640

Purpose

Retroviral expression of mouse CD28 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (Cd28 Mouse)

Use

Retroviral; mammalian expression

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Human a3 nicotinic receptor subunit EM construct

Plasmid

#163960

Purpose

pEZT-BM vector with alpha3 gene after CMV promoter, published EM construct

Depositor

Ryan Hibbs

Article

Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do

Insert

Human alpha3 nicotinic acetylcholine receptor subunit EM construct

Use

Baculovirus (bacmam) production and mammalian exp…

Tags

apocytochrome b(562)RIL (BRIL)

Expression

Mammalian

Mutation

Asn348-Ser402 replaced with apocytochrome b(562)R…

Promoter

CMV

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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