We narrowed to 1,374 results for: ccdb gene

1. nuclear cpVenus control

   Plasmid

   #186790

   Purpose

   Identical to nuclear NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   nuclear cpVenus control
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. BPK2014-AaCas12b

   Plasmid

   #121949

   Purpose

   E. coli expression, protein purification

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   AaCas12b
   

   Use

   Tags

   10xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDD317 (Pmyo-2::GFP + SEC)

   Plasmid

   #73344

   Purpose

   SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Hibshman et al BMC Biol. 2021 Dec 14;19(1):263. doi: 10

   Insert

   Pmyo-2::GFP + SEC
   

   Use

   CRISPR and Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   myo-2

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOCC154

   Plasmid

   #118902

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
   

   Use

   Tags

   TwinStrep, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. p413TEF1-ATR1(pYL573)

   Plasmid

   #178288

   Purpose

   Expresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Insert

   ATR1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOCC153

   Plasmid

   #118901

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   TwinStrep, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pdestMB14

   Plasmid

   #26415

   Depositor

   Marc Vidal

   Article

   Dupuy et al Genome Res. 2004 Oct;14(10B):2169-75.

   Insert

   attR4-ccdB-attR2
   

   Use

   Tags

   Expression

   Bacterial and Worm

   Mutation

   Promoter

   Available Since

   April 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pdestDD03

   Plasmid

   #26414

   Depositor

   Marc Vidal

   Article

   Dupuy et al Genome Res. 2004 Oct;14(10B):2169-75.

   Insert

   attR4-ccdB-attR2 cassette
   

   Use

   Tags

   Expression

   Bacterial and Worm

   Mutation

   Promoter

   Available Since

   April 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. BPK2014-AmCas12b

   Plasmid

   #121951

   Purpose

   E. coli expression, protein purification

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   AmCas12b
   

   Use

   Tags

   10xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG415Gal_Gcn4 WW+GFP

    Plasmid

    #233003

    Purpose

    Galactose iduced expression of Gcn4 WW+GFP in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 WW+
    

    Use

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    E109N, S117D, T121W, N126W, T132W

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG415Gal_Gcn4 Aro2+GFP

    Plasmid

    #233001

    Purpose

    Galactose iduced expression of Gcn4 Aro2+GFP in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 Aro2+
    

    Use

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    N116F, S117E, E119N, P129Q, T132Y

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG415Gal_Gcn4 solvvol

    Plasmid

    #232976

    Purpose

    Galactose iduced expression of Gcn4 solvvol in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 solvvol
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG415Gal_Gcn4 S/ArotoL

    Plasmid

    #232979

    Purpose

    Galactose iduced expression of Gcn4 S/ArotoL in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 S/ArotoL
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    S101L, S104L, F108L, Y110L, S117L, S136L, S144L

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAG415Gal_Gcn4 ILVtoED WHA

    Plasmid

    #232995

    Purpose

    Galactose iduced expression of Gcn4 ILVtoED W+HAin yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoED W+
    

    Use

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    N126W, I128E, V130E, V135E, L137D, I142D

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG415Gal_Gcn4 9acidAHA

    Plasmid

    #232997

    Purpose

    Galactose iduced expression of Gcn4 9acidAHA in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 9acidA
    

    Use

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG415Gal_Gcn4 9acidAGFP

    Plasmid

    #233009

    Purpose

    Galactose iduced expression of Gcn4 9acidAGFP in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 9acidA
    

    Use

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG415Gal_Gcn4 Aro3+GFP

    Plasmid

    #233002

    Purpose

    Galactose iduced expression of Gcn4 Aro3+GFP in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 Aro3+
    

    Use

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    D103Y, N112T, L113Y, S117F, K140R

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG415Gal_Gcn4 ILVtoED WGFP

    Plasmid

    #233007

    Purpose

    Galactose iduced expression of Gcn4 LVtoED W+GFPin yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoED W+
    

    Use

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    N126W, I128E, V130E, V135E, L137D, I142D

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEN_TT BirA*-3xFLAG-NUP37

    Plasmid

    #192311

    Purpose

    Gateway entry vector for an inducible 3xFLAG-BirA* 3xFLAG-NUP37

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    BirA*-3xFLAG-NUP37 (NUP37 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOCC111

    Plasmid

    #118906

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal sortase site (LPETGg) and HIS6

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-LPETGg-HIS6-stop-HindIII cassette
    

    Use

    Tags

    LPETGg, HIS6

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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