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pAG415Gal_Gcn4 WW+

Plasmid

#232984

Purpose

Galactose iduced expression of Gcn4 WW+ in yeast

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 WW+

Use

Tags

TEV cleavage site

Expression

Yeast

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 F108A+

Plasmid

#232974

Purpose

Galactose iduced expression of Gcn4 F108A in yeast

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 F108A

Use

Tags

TEV cleavage site

Expression

Yeast

Mutation

F108A, T121L, A141P

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 ILVtoAS

Plasmid

#232959

Purpose

Galactose iduced expression of Gcn4 ILVtoAS in yeast

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 ILVtoAS

Use

Tags

TEV cleavage site

Expression

Yeast

Mutation

L123S, I128S, V130A, V135A, L137S, I142S

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WT 44merGFP

Plasmid

#233000

Purpose

Galactose iduced expression of Gcn4 WT 44merGFP in yeast

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 WT 44mer

Use

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WT 30mer

Plasmid

#232986

Purpose

Galactose iduced expression of Gcn4 WT 30mer in yeast

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 WT 30mer

Use

Tags

TEV cleavage site

Expression

Yeast

Mutation

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-APP-CTF

Plasmid

#181732

Purpose

Galactose inducible expression of APP-CTF

Depositor

Alejandro Chavez

Insert

APP-CTF (APP Human)

Use

Tags

Expression

Yeast

Mutation

C-terminal fragment (aa 671-770)

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEcgRNA

Plasmid

#166581

Purpose

CRISPR-Cas9-assisted genome editing in Escherichia coli

Depositor

Sheng Yang

Article

Li et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB701NL3F10H

Plasmid

#141288

Purpose

No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, Tunicamycin R for plant)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Type

Empty backbone

Use

Luciferase; Gateway

Tags

FLAG (x3), HIS (x10), and NLUC

Expression

Plant

Mutation

Promoter

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pOCC82

Plasmid

#118862

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for untagged recombinant protein

Depositor

PEPC MPI CBG, Marc Bickle

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-NotI-ccdB-AscI-stop-HindIII cassette

Use

Tags

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB501NL3F10H

Plasmid

#141286

Purpose

No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, HygR for plant)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Type

Empty backbone

Use

Luciferase; Gateway

Tags

FLAG (x3), HIS (x10), and NLUC

Expression

Plant

Mutation

Promoter

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB601NL3F10H

Plasmid

#141287

Purpose

No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, BASTA R for plant)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Type

Empty backbone

Use

Luciferase; Gateway

Tags

FLAG (x3), HIS (x10), and NLUC

Expression

Plant

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB401NL3F10H

Plasmid

#141285

Purpose

No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, KmR for plant)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Type

Empty backbone

Use

Luciferase; Gateway

Tags

FLAG (x3), HIS (x10), and NLUC

Expression

Plant

Mutation

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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p415PGK1-AtMAX1(pYL759)

Plasmid

#178289

Purpose

Expresses MAX1 from A. thaliana (AtMAX1) in Saccharomyces cerevisiae. Centromeric LEU, PPGK1-AtMAX1-Tpho5

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

AtMAX1

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK1

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-GaCYP722C(pYL777)

Plasmid

#178290

Purpose

Expresses GaCYP722C from G. arboreum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-GaCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

GaCYP722C

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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p416PGK1-OsCYP711A2(pYL770)

Plasmid

#178301

Purpose

Expresses OsCYP711A2 from O. sativa in Saccharomyces cerevisiae. Centromeric URA, PPGK1-OsCYP711A2-Tpho5

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

OsCYP711A2

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK1

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-CaCYP722C(pYL803)

Plasmid

#178291

Purpose

Expresses CaCYP722C from C. annuum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-CaCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

CaCYP722C

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-LjCYP722C(pYL795)

Plasmid

#178292

Purpose

Expresses LjCYP722C from L. japonicus in Saccharomyces cerevisiae. Centromeric TRP, PGPD-LjCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

LjCYP722C

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG414GPD-VuCYP722C(pYL1070)

Plasmid

#178293

Purpose

Expresses VuCYP722C from V. unguiculata in Saccharomyces cerevisiae. Centromeric TRP, PGPD-VuCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

VuCYP722C

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-SlCYP722C(pYL1071)

Plasmid

#178294

Purpose

Expresses SlCYP722C from S. lycopersicum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-SlCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

SlCYP722C

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG414GPD-TpCYP722C(pYL851)

Plasmid

#178295

Purpose

Expresses TpCYP722C from T. pratense in Saccharomyces cerevisiae. Centromeric TRP, PGPD-TpCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

TpCYP722C

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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