Addgene

We narrowed to 3,048 results for: golden gate

Showing: 801 - 820 of 3048 results

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pICH47742:: pRPS5a-PE2max-NC::tHSP

Plasmid

#227694

Purpose

Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Insert

PE2max-NC being driven by pRPS5a and terminated by tHSP

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

pRPS5a

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pICH47742::p35S-PE2max-NC::tEURb7

Plasmid

#227693

Purpose

Golden Gate level 1, position 2, PE2max-NC expression cassette.

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Insert

PE2max-NC being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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MSGV Mouse Acceptor PGK NGFR

Plasmid

#128482

Purpose

A TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR expressing NGFR ref Biotechniques. 2015 Mar 1;58(3):135-9

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

MSCV

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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BB1_12_pGAP

Plasmid

#98498

Purpose

BB1 with insert: promoter PGAP

Depositor

Michael Sauer, Diethard Mattanovich, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

GAP promoter

Use

Tags

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLPE1

Plasmid

#209981

Purpose

Contains Level 0 Part: E. coli shuttle part (pMB1_ampR_B0017) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

E. coli shuttle part (pMB1_ampR_B0017)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLPE2

Plasmid

#209982

Purpose

Contains Level 0 Part: E. coli shuttle part (ampR_B0017) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

E. coli shuttle part (ampR_B0017)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLPE3

Plasmid

#209985

Purpose

Contains Level 0* Part: E. coli shuttle part (pMB1_cat) for the construction of Level 2 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

E. coli shuttle part (pMB1_cat)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLPP5

Plasmid

#209967

Purpose

Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P_sppQ)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLPP6

Plasmid

#209968

Purpose

Contains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertion

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter Dropout (sfGFP)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLP-mScarlet-I

Plasmid

#209993

Purpose

Contains Level 0 Part: Coding sequences (mScarlet-I) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher, Georg Fritz

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

mScarlet-I

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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BB3rN_14*

Plasmid

#98550

Purpose

empty BB3 for insertion and overexpression of 1 gene in P. pastoris

Depositor

Michael Sauer, Diethard Mattanovich, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB3aZ_14*

Plasmid

#98542

Purpose

empty BB3 for insertion and overexpression of 1 gene in P. pastoris

Depositor

Michael Sauer, Diethard Mattanovich, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3aN_14*

Plasmid

#98548

Purpose

empty BB3 for insertion and overexpression of 1 gene in P. pastoris

Depositor

Michael Sauer, Diethard Mattanovich, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_34_RPS25Att

Plasmid

#98526

Purpose

BB1 with insert: RPS25Att

Depositor

Michael Sauer, Diethard Mattanovich, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

RPS25Att

Use

Tags

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_23_eGFP

Plasmid

#98518

Purpose

BB1 with insert: CDS eGFP

Depositor

Michael Sauer, Diethard Mattanovich, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

eGFP

Use

Tags

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB3aK_14*

Plasmid

#98529

Purpose

empty BB3 for insertion and overexpression of 1 gene in P. pastoris

Depositor

Michael Sauer, Diethard Mattanovich, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_12_pLAT1

Plasmid

#98509

Purpose

BB1 with insert: promoter PLAT1

Depositor

Michael Sauer, Diethard Mattanovich, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

LAT1 promoter

Use

Tags

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_12_pPFK300

Plasmid

#98510

Purpose

BB1 with insert: promoter PPpPfk

Depositor

Michael Sauer, Diethard Mattanovich, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

PpPfk promoter

Use

Tags

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_12_pDAS2

Plasmid

#98513

Purpose

BB1 with insert: promoter PDAS2

Depositor

Michael Sauer, Diethard Mattanovich, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

DAS2 promoter

Use

Tags

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_12_pRPL2A

Plasmid

#98508

Purpose

BB1 with insert: promoter PRPL2A

Depositor

Michael Sauer, Diethard Mattanovich, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

RPL2A promoter

Use

Tags

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge