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LI C-D YFP noATG

Plasmid

#54437

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

YFP Venus without Start codon

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LI D-E C-NES c1

Plasmid

#54429

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

NES of heat stable inhibitor (PKI)

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pFN1-Aad9

Plasmid

#244449

Purpose

A shuttle plasmid compatible with Fusobacterium nucleatum ATCC10953 or ATCC25586, carrying a spectinomycin resistance marker (aadA gene).

Depositor

Xue Liu

Inserts

pFn1 Fn ori
Aad9

Expression

Bacterial

Available since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ3538 eforRed-targeting sgRNA

Plasmid

#239455

Purpose

guide for eforRed

Depositor

Stanley Qi

Publication

Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

Insert

guide targeting eforRed

Use

CRISPR; Cell-free system using bacterial extract

Expression

Bacterial

Promoter

J23119

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-FAB(ER-LD)

Plasmid

#214420

Purpose

Detection of ER-lipid droplet contact site

Depositor

Chi-Lun Chang

Publication

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-FRB-V5-ER_IRES_CFAST-20nm-Halo-6xHp

Use

Lentiviral

Tags

CFAST-Halo and RspAN-FRB-V5

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pNL1 Ecarin

Plasmid

#106289

Purpose

Expresses Ecarin in Ecoli off a T7 promoter in the NEB control cloning vector

Depositor

James Collins

Publication

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

Ecarin

Expression

Bacterial

Promoter

T7

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pBP-pET-RBS

Plasmid

#72981

Purpose

PET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)

Depositor

Paul Freemont

Publication

Moore et al ACS Synth Biol. 2016 May 2.

Insert

pET RBS derived from pET vectors

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-lacZ

Plasmid

#72948

Purpose

Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning

Depositor

Paul Freemont

Publication

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 0 - Bioparts

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PMP34-mApple

Plasmid

#214417

Purpose

Peroxisome localization of PMP34 fused to mApple

Depositor

Chi-Lun Chang

Publication

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

PMP34 (SLC25A17 Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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LT2_15_UPRpro_X2site

Plasmid

#194427

Purpose

Plasmid for genome integration in X2 site expressing UPRpro - Sensor for Unfolded Protein Response

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

UPRpro

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Mutation

NA

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT2_32_PyruEth_X2site

Plasmid

#194428

Purpose

Plasmid for genome integration in X2 site expressing PyruEth (Pyruvate and Ethanol consumption sensors)

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

PyruEth

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Mutation

NA

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_77_TU1of2-6_HOhomology

Plasmid

#194416

Purpose

TU1 backbone (for TU #1 for plasmid with 2-6 TUs) with HO homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_79_TU2of3-6_HOhomology

Plasmid

#194418

Purpose

TU2 backbone (for TU #2 for plasmid with 3-6 TUs) with HO homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_81_TU3of4-6_HOhomology

Plasmid

#194420

Purpose

TU3 backbone (for TU #3 for plasmid with 4-6 TUs) with HO homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_83_TU4of5-6_HOhomology

Plasmid

#194422

Purpose

TU4 backbone (for TU #4 for plasmid with 5-6 TUs) with HO homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_63_TU1of2-6_X2homology

Plasmid

#194405

Purpose

TU1 backbone (for TU #1 for plasmid with 2-6 TUs) with X2 homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_65_TU2of3-6_X2homology

Plasmid

#194407

Purpose

TU2 backbone (for TU #2 for plasmid with 3-6 TUs) with X2 homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_67_TU3of4-6_X2homology

Plasmid

#194409

Purpose

TU3 backbone (for TU #3 for plasmid with 4-6 TUs) with X2 homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_69_TU4of5-6_X2homology

Plasmid

#194411

Purpose

TU4 backbone (for TU #4 for plasmid with 5-6 TUs) with X2 homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBP-Tag_linker

Plasmid

#72982

Purpose

N-terminal linker with pET-RBS (5' GTAC/3' TAAA fusion)

Depositor

Paul Freemont

Publication

Moore et al ACS Synth Biol. 2016 May 2.

Insert

N-terminal linker with pET-RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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