We narrowed to 5,482 results for: pcas

1. pCas3-Apr

   Plasmid

   #208000

   Purpose

   Expresses all necessary components of Type I-C CRISPR-Cas system

   Depositor

   Pablo Ivan Nikel

   Article

   Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

   Inserts

   RhaR (rhaR )
   RhaS (rhaS )
   pRhaBAD
   I-C crRNA
   Cas3
   Cas5
   Cas8
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG_SRGAP2A_GAP(R527L_K566A)

   Plasmid

   #222617

   Purpose

   Expresses full length SRGAP2 with R527L, K566A mt in the Rac1-specific GAP domain that abrogates the GTPase activating function of SRGAP2 without locking the GAP-domain in a form bound to Rac1-GDP.

   Depositor

   Franck Polleux

   Article

   Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

   Insert

   SRGAP2 (SRGAP2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Point mutations R527L and K566A (Rac1-specific GA…

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSAVED-CHAT_PCaspase

   Plasmid

   #214043

   Purpose

   Bacterial expression plasmid for SAVED-CHAT and PCaspase N-terminal fragment (aa 1-153) from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lacUV5 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSAVED-CHAT_PCaspase_PCi

   Plasmid

   #214049

   Purpose

   Bacterial expression plasmid for SAVED-CHAT, PCaspase, and PCi from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT-Pcaspase-PCi
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lacUV5 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAT.482

   Plasmid

   #203952

   Purpose

   Spectinomycin-based RSF1010 acceptor Level T

   Depositor

   Alistair McCormick

   Article

   Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG_ALX4-Flag-HA

   Plasmid

   #215028

   Purpose

   Mammalian expression of human ALX4

   Depositor

   Joanna Wysocka

   Article

   Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

   Insert

   ALX homeobox 4 (ALX4 Human)
   

   Use

   Tags

   Flag-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-ORF3cStrep

   Plasmid

   #208728

   Purpose

   Expresses ORF3c-Strep in mammalian cells

   Depositor

   Andrew Firth

   Insert

   ORF3c (ORF3a )
   

   Use

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-ORF3cHA

   Plasmid

   #208653

   Purpose

   Express ORF3c-HA in mammalian cells

   Depositor

   Andrew Firth

   Insert

   ORF3c (ORF3a )
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAB1-YFP-GW

   Plasmid

   #209363

   Purpose

   Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis CAB1 promoter driving expression of N-terminal YFP fusion protein.

   Depositor

   Lucia Strader

   Article

   Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL0_156 [PcatP]

    Plasmid

    #210678

    Purpose

    Level 0 part

    Depositor

    Charlie Gilbert, Cultivarium Tools, Nili Ostrov

    Insert

    PcatP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LLP781_SnoopCatcher-KRAB-CO

    Plasmid

    #211774

    Purpose

    SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Traptavidin-KRAB
    

    Use

    Tags

    3xV5

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect KRAB e…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG/ATP11C-c-HA

    Plasmid

    #209222

    Purpose

    Mammalian expression of ATP11C isoform c

    Depositor

    Hye-Won Shin

    Article

    Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

    Insert

    ATP11C isoform c (ATP11C Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG/ATP11C-b-HA

    Plasmid

    #209221

    Purpose

    Mammalian expression of ATP11C isoform b

    Depositor

    Hye-Won Shin

    Article

    Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

    Insert

    ATP11C isoform b (ATP11C Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. cacnb1b pCAGGS

    Plasmid

    #206102

    Purpose

    expression of rat beta1b calcium channel auxillary subunit

    Depositor

    Annette Dolphin

    Insert

    cacnb1b (Cacnb1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/B-actin

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cav2.2 pCAGGS

    Plasmid

    #206101

    Purpose

    expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

    Depositor

    Annette Dolphin

    Article

    Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/B-actin

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG/ATP8B3-HA

    Plasmid

    #209211

    Purpose

    Mammalian expression of ATP8B3

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    ATP8B3 (ATP8B3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. B-HeFSpCas9-A695Q

    Plasmid

    #207368

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A695Q
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, R661A, K848A, Q926A, K1003A, R1060A, amino…

    Promoter

    CBh

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. B-HeFSpCas9-A926Q

    Plasmid

    #207370

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A926Q
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, K1003A, R1060A, amino…

    Promoter

    CBh

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. B-evoSpCas9-E526K

    Plasmid

    #207365

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9-E526K
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    M495V, Y515N, R661Q, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. B-evoSpCas9-V495M

    Plasmid

    #207363

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9-V495M
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Y515N, K526E, R661Q, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge