We narrowed to 19,449 results for: Tec

Showing: 8181 - 8200 of 19449 results

Results per page:

Sort results by:

Lenti AID-BEmax

Plasmid

#157950

Purpose

Lentiviral expression of AID-BEmax

Depositor

Dali Li

Article

Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Lenti AID-BEmax

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AID-BE4max-P2A-GFP

Plasmid

#157948

Purpose

Mammalian expression of AID-BE4max-P2A-GFP

Depositor

Dali Li

Article

Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

AID-BE4max

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMonAID_nCas9-PmCDA_Hyg_ALS

Plasmid

#91693

Purpose

Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls

Depositor

Akihiko Kondo

Article

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

2x35S

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
SpCas9-NRCH-SsAPOBEC3B

Plasmid

#198550

Purpose

Expresses human codon-optimized SpCas9-NRCH-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NRCH-SsAPOBEC3B-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NRCH-SsAPOBEC3B

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPM-miR30-DDX58-ts3

Plasmid

#174243

Purpose

DDX58 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

DDX58 shRNA (RIGI Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CaMKII-iGECI-NES

Plasmid

#160424

Purpose

AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI with a nuclear export signal sequence

Depositor

Vladislav Verkhusha

Article

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI-NES

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti PA-mCit

Plasmid

#113451

Purpose

Lentiviral ProteinA-mCitrine expression vector

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-mCitrine

Use

Lentiviral

Expression

Mammalian

Promoter

hUBbC

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLCHKO_HPRT1_exon2_1_Lb

Plasmid

#155051

Purpose

Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA for deletion of HPRT1 exon 2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Cerulean3-Cerulean3-FLARE-Cameleon

Plasmid

#123353

Purpose

Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Cerulean3-Cerulean3-FLARE-Cameleon

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-HLA-cmyc-natT1R2

Plasmid

#113946

Purpose

mammalian expression plasmid for c-myc-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

Signal/leader sequence from HLA class I histocomp…

Expression

Mammalian

Mutation

residues 22-839

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
SpCas9-NG-APOBEC-nCas9-Ung

Plasmid

#198539

Purpose

Expresses human codon-optimized SpCas9-NG-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NG-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NG-APOBEC-nCas9-Ung

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
non-targeting control gRNA (BRDN0001145598)

Plasmid

#80173

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TNIK gRNA (BRDN0001146045)

Plasmid

#75849

Purpose

3rd generation lentiviral gRNA plasmid targeting human TNIK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TNIK (TNIK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-hA3H-BE3

Plasmid

#113416

Purpose

Expresses hA3H-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3H-BE3 (APOBEC3H Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2 Beta1b GFP

Plasmid

#89893

Purpose

Mammalian expression of Beta1b GFP

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1

Insert

Cacnb1 (Cacnb1 Rat)

Tags

GFP

Expression

Mammalian

Mutation

GFP sequence inserted just before the Stop codon

Promoter

adenovirus major late promoter

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST_FLAG-HUWE1_delta169-189

Plasmid

#187136

Purpose

mammalian cell expression of FLAG-HUWE1 delta169-189

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

delta169-189

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TV-hPITX3-forward

Plasmid

#22073

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

PITX3 (ASMD Human)

Use

Cre/Lox; Zfn donor

Tags

eGFP

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
TrHKI-pGFPN3

Plasmid

#21918

Depositor

Hossein Ardehali

Article

Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

Insert

Truncated human HKI (lacking exon 1 sequence that encodes for the mitochondrial binding domain) in pGFP-N3 plasmid (HK1 Human)

Tags

GFP

Expression

Mammalian

Mutation

This is the truncated human HKI cDNA (lacking exo…

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-mCherry-mCherry-FLARE-AKAR

Plasmid

#123333

Purpose

Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

mCherry-mCherry-FLARE-AKAR

Tags

6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLCHKO_HPRT1_exon3_1_Lb

Plasmid

#155053

Purpose

Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA for deletion of HPRT1 exon 3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge