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dsRedm-Rab21

Plasmid

#83425

Purpose

Full-length mouse Rab21 (wildtype)

Depositor

Johanna Ivaska

Publication

Pellinen et al Dev Cell. 2008 Sep;15(3):371-85. doi: 10

Insert

Rab21 (Rab21 Mouse)

Tags

dsRed-monomer

Expression

Mammalian

Promoter

CMV

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Nicastrin-CD4d3+4-bio

Plasmid

#73106

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Nicastrin with rat CD4d3+4-bio

Depositor

Gavin Wright

Publication

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Nicastrin (NCSTN Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pGK:TRL-EFS:iRFP670

Plasmid

#68475

Purpose

Lentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

tTR-KRAB-2A-rtTA3-2A-Luciferase
iRFP670

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

EFS and pGK

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pIK127

Plasmid

#65631

Purpose

Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter.

Depositor

Iskra Katic

Publication

Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

Insert

Peft-3::gfp::h2b::tbb-2 3'UTR

Expression

Worm

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-act5cB-GAL4QF

Plasmid

#61309

Purpose

Expresses chimeric transactivator GAL4_DBD:QF2w_AD in Drosophila

Depositor

Christopher Potter

Publication

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

GAL4_DBD::QF2w_AD

Expression

Insect

Mutation

Changed EK on C-terminus of QF2 to KKKK

Available since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNAIIIB cdc42 WT

Plasmid

#61635

Purpose

mammalian expression of cdc42 wt

Depositor

JS Gutkind

Publication

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

cdc42 (CDC42 Human)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
mEmerald-MyosinIIC-C-18

Plasmid

#54194

Purpose

Localization: Cytoskeleton, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Publication

Ebrahim et al Curr Biol. 2013 Apr 22;23(8):731-6. doi:

Insert

MyosinIIC

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3-V5-Lin28A

Plasmid

#51387

Depositor

Narry Kim

Publication

Heo et al Cell. 2009 Aug 21;138(4):696-708. doi: 1

Insert

Lin28A (LIN28A Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pAO122 (Apr LEU2 MRC1)

Plasmid

#41923

Depositor

Stephen Elledge

Publication

Osborn et al Genes Dev. 2003 Jul 15;17(14):1755-67.

Insert

Mediator of the Replication Checkpoint

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
f2H ∂HD (272)

Plasmid

#24428

Depositor

Steven Rosen

Publication

Morimoto-Tomita et al Neoplasia. 2005 Nov . 7(11):1001-10.

Insert

SULF2 (SULF2 Human)

Tags

FLAG, His, and Myc

Expression

Mammalian

Mutation

AA 418-716 deleted, 23AA N-terminal truncation

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
f1H ∂HD (267)

Plasmid

#24426

Depositor

Steven Rosen

Publication

Morimoto-Tomita et al Neoplasia. 2005 Nov . 7(11):1001-10.

Insert

SULF1 (SULF1 Human)

Tags

FLAG, His, and Myc

Expression

Mammalian

Mutation

AA 416-726 deleted, 22AA N-terminal truncation

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pEMS1505

Plasmid

#29300

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple34 (CLDN5 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available since

Nov. 4, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pGL3-RbE2Fmut

Plasmid

#32391

Depositor

Julien Sage

Publication

Burkhart et al Mol Cell Biol. 2010 Apr;30(7):1729-45. E

Insert

Rb promoter (Rb1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated E2F binding site: TTTCCCGC --> TTTACATC

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-nMyc-wt_3'UTR

Plasmid

#31858

Depositor

Robert Blelloch

Publication

Melton et al Nature. 2010 Feb 4;463(7281):621-6. Epub

Insert

nMyc wild-type 3'UTR

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

SV40

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shRalA-2

Plasmid

#15258

Depositor

William Hahn

Publication

Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

Insert

v-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pLenti-EF1a-HIF2a dPA (P405A, P531A)

Plasmid

#232954

Purpose

Stably express a constitutively stable version of HIF2a (HIF2a dPA)

Depositor

William Kaelin

Publication

Jiang et al Cell. 2025 Feb 25:S0092-8674(25)00156-4.

Insert

HIF2a (EPAS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

P405A, P531A

Promoter

EF1a

Available since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP8_nostop

Plasmid

#149311

Purpose

Gateway-compatible Entry vector, with insert of NSP8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP8 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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ECE1-His-bio

Plasmid

#53361

Purpose

Expresses full-length Endothelin converting enzyme 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

Depositor

Gavin Wright

Publication

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

ECE1 (ECE1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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INSR-bio-His

Plasmid

#53341

Purpose

Expresses full-length Insulin receptor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Publication

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

INSR (INSR Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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APOH-bio-His

Plasmid

#52177

Purpose

Expresses full-length Beta-2-glycoprotein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Publication

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

APOH (APOH Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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