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pAc5.1B-lambdaN-HA-DmThor-del76-85-V5His6_T

Plasmid

#147613

Purpose

Insect Expression of DmThor-del76-85

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-del76-85 (Thor Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HseIF4G_571-676-GB1_AG

Plasmid

#148745

Purpose

Bacterial Expression of HseIF4G_571-676

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G_571-676 (EIF4G1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676-GB1_AD

Plasmid

#148512

Purpose

Bacterial Expression of DmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676 (eIF4G1 Fly)

Expression

Bacterial

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Flnc

Plasmid

#174870

Purpose

CRISPR vector for generating Flnc STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Flnc (Flnc Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Coexpressed Free PcPTS

Plasmid

#182483

Purpose

Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and patchoulol synthase from Pogostemon cablin (PcPTS; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

Inserts

FPPS
PTS

Use

Synthetic Biology; Metabolic engineering

Expression

Yeast

Promoter

GAL10 and GAL7

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Linked Free NES (AG4TGGA)2

Plasmid

#182488

Purpose

Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence (AG4TGGA)2 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

Insert

FPPS-NES

Use

Synthetic Biology; Metabolic engineering

Expression

Yeast

Promoter

GAL10

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Linked Free (PT)4P

Plasmid

#182490

Purpose

Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence PTPTPTPTP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

Insert

FPPS-NES

Use

Synthetic Biology; Metabolic engineering

Expression

Yeast

Promoter

GAL10

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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ABHD2-3'UTR-Del-1

Plasmid

#190095

Purpose

examine the activity of ABHD2 3'UTR

Depositor

Bowen Liu

Article

Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

Insert

ABHD2 3' UTR delete-1 (ABHD2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

deleted the base pairs 6704-6709 refer to NM_0070…

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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ABHD2-3'UTR-Del-2

Plasmid

#190096

Purpose

examine the activity of ABHD2 3'UTR

Depositor

Bowen Liu

Article

Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

Insert

ABHD2 3' UTR delete-2 (ABHD2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

deleted the base pairs 7122-7129 refer to NM_0070…

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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DDX11-1

Plasmid

#155503

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX11 (DDX11 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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