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We narrowed to 9,707 results for: crispr plasmids

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Showing: 8201 - 8220 of 9707 results

  3. pLenti-nSpCas9-evoCDA1

    Plasmid
    #209042
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    evoCDA1-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209042-plasmid-map-sequence-id-413615

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  5. pLenti-nSpCas9-APOBEC3A

    Plasmid
    #209040
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    APOBEC3A-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209040-plasmid-map-sequence-id-413592

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  6. pLenti-nSpCas9-TadA8e

    Plasmid
    #209044
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    TadA8e-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209044-plasmid-map-sequence-id-413636

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  7. pBFC1171

    Plasmid
    #231994
    Purpose
    CRISPRi-ART crRNA only plasmid encoding crystal violet-inducible crRNA (for dRfxCas13d) with 2xBsaI spacer cloning site
    Depositor
    Brady Cress
    Article
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Insert
    crRNA of dRfxCas13d
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    pJEx (Jungle Express)
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    231994-plasmid-map-sequence-id-468506

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  8. pLenti-nSpCas9-NoCBE

    Plasmid
    #209038
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase) linked to ssDNA binding domain from RAD51 and uracil DNA glycosylase inhibitor (UGI)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209038-plasmid-map-sequence-id-413620

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  9. pLenti-nSpCas9-BEACON2

    Plasmid
    #209041
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where BEACON2 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    BEACON2-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209041-plasmid-map-sequence-id-419488

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  10. pLenti-nSpCas9-TadA8.20m

    Plasmid
    #209045
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8.20m is fused to the N-terminus of SpCas9(D10A)-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    TadA8.20m-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209045-plasmid-map-sequence-id-413618

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  13. peSCBE3-NG-HF1-Hypa

    Plasmid
    #218166
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-HF1-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218166-plasmid-map-sequence-id-433270

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  16. pX551-miniCMV-SpCas9D10A nickase

    Plasmid
    #216735
    Purpose
    Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    T7 and mini-CMV
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216735-plasmid-map-sequence-id-430321

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  17. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA

    Plasmid
    #136652
    Purpose
    Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136652-plasmid-map-sequence-id-273328

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  18. pWZ194

    Plasmid
    #163640
    Purpose
    rps-27>DHB::2xmKate2-P2A-H2B::GFP expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I
    Depositor
    David Matus
    Article
    Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1
    Insert
    DHB::2mKate2-P2A-H2B::GFP::3xHA (his-58 Nematode, Synthetic)
    Use
    CRISPR
    Tags
    2xmKate2 and GFP
    Expression
    Worm
    Promoter
    rps-27
    Available Since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    163640-plasmid-map-sequence-id-324064

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  19. pAWK61

    Plasmid
    #163642
    Purpose
    rps-27>DHB::GFP-P2A-H2B::2xmKate2 expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I
    Depositor
    David Matus
    Article
    Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1
    Insert
    DHB::GFP-P2A-H2B::2xmKate2::3xHA (his-58 Human, Nematode)
    Use
    CRISPR
    Tags
    2xmKate2 and GFP
    Expression
    Worm
    Promoter
    rps-27
    Available Since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    163642-plasmid-map-sequence-id-362086

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  20. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

    Plasmid
    #136659
    Purpose
    Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:TH1477 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136659-plasmid-map-sequence-id-273359

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Showing: 8201 - 8220 of 9707 results