Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 9,351 results for: sacs

Showing: 8201 - 8220 of 9351 results

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pLD-puro-Cc-SARS-CoV-2_Spike_Lambda-VA

Plasmid

#191220

Purpose

Lentiviral expression of SARS-CoV-2 Spike_Lambda in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

SPIKE_SARS2_Lambda (S SARS CoV-2)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

G75V T76I Del 246-252 L452Q F490S D614G T859N

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-IFIT5_WT-VA

Plasmid

#191221

Purpose

Lentiviral expression of human IFIT5_WT in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-IFIT5_K160R-VA

Plasmid

#191222

Purpose

Lentiviral expression of human IFIT5_K160R in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

changed Lysine 160 to Arginine (K160R)

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-SARS-CoV-2_NSP3-VA

Plasmid

#191214

Purpose

Lentiviral expression of SARS-CoV-2 NSP3 in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

Non structural protein 3 (ORF1ab SARS CoV-2)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLViN-iRFP670-α-cateninA+ΔβH

Plasmid

#229707

Purpose

Lentiviral expression of iRFP670-alpha-cateninA+delta-beta-H in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Mutation

amino acids 670-673, RAIM--> GSGS

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 GST-hUSP27X K181E

Plasmid

#225714

Purpose

Bacterial expression of USP27X (K181E) with GST tag

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X K181E (USP27X Human)

Tags

GST

Expression

Bacterial

Mutation

K181E

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro 3XFLAG-hATXN7L3

Plasmid

#225725

Purpose

Transfection of ATXN7L3 with 3XFLAG tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

ATXN7L3 (ATXN7L3 Human)

Tags

3XFLAG

Expression

Mammalian

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X S404N

Plasmid

#225724

Purpose

Transfection of USP27X (S404N) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X S404N (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

S404N

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X F313V

Plasmid

#225722

Purpose

Transfection of USP27X (F313V) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X F313V (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

F313V

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X K181E

Plasmid

#225721

Purpose

Transfection of USP27X (K181E) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X K181E (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

K181E

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X Y144C

Plasmid

#225720

Purpose

Transfection of USP27X (Y144C) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X Y144C (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

Y144C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X G76S

Plasmid

#225719

Purpose

Transfection of USP27X (G76S) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X G76S (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

G76S

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 GST-hUSP27X S404N

Plasmid

#225717

Purpose

Bacterial expression of USP27X (S404N) with GST tag

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X S404N (USP27X Human)

Tags

GST

Expression

Bacterial

Mutation

S404N

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 GST-hUSP27X Y381H

Plasmid

#225716

Purpose

Bacterial expression of USP27X (Y381H) with GST tag

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X Y381H (USP27X Human)

Tags

GST

Expression

Bacterial

Mutation

Y381H

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 GST-hUSP27X F313V

Plasmid

#225715

Purpose

Bacterial expression of USP27X (F313V) with GST tag

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X F313V (USP27X Human)

Tags

GST

Expression

Bacterial

Mutation

F313V

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 GST-hUSP27X Y144C

Plasmid

#225713

Purpose

Bacterial expression of USP27X (Y144C) with GST tag

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X Y144C (USP27X Human)

Tags

GST

Expression

Bacterial

Mutation

Y144C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 GST-hUSP27X G76S

Plasmid

#225712

Purpose

Bacterial expression of USP27X (G76S) with GST tag

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X G76S (USP27X Human)

Tags

GST

Expression

Bacterial

Mutation

G76S

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 GST-hUSP27X C87A

Plasmid

#225711

Purpose

Bacterial expression of USP27X (C87A) with GST tag

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X C87A (USP27X Human)

Tags

GST

Expression

Bacterial

Mutation

C87A, inactivates deubiquitylase

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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NYESO beta/alpha into TRAC HDRT Source (pTR 169)

Plasmid

#112021

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO beta/alpha into TRAC HDRT

Use

CRISPR and Synthetic Biology

Available Since

Sept. 1, 2018

Availability

Academic Institutions and Nonprofits only

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P3-dCas9-Tet1-GFP-Puro

Plasmid

#190728

Purpose

Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

Depositor

Maja Jagodic

Article

Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

Insert

dCas9-tet1

Use

CRISPR

Tags

3XFLAG and SV40 NLS

Expression

Mammalian

Mutation

Changed Pro434 codon from CCG to CCC and Gly468 f…

Promoter

pCAG

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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