We narrowed to 16,131 results for: form

1. pCSD_2XNLS-SpCas9MT3-NLS-3XHA-2XNLS-NmdCas9-2XNLS

   Plasmid

   #107323

   Purpose

   Expresses SpCas9 (R1335K) fused to dNmCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9-NmCas9 fusion
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SpCas9 (R1335K) is fused to nuclease dead NmCas9 …

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV 3xFLAG-LC3A G120

   Plasmid

   #123091

   Purpose

   Expresses 3xFLAG-LC3A G120 in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Deleted amino acid 121. Stop codon after G120.

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. His10-B1 Integrin

   Plasmid

   #186139

   Purpose

   For expression of recombinant his10-beta1 integrin cytosolic domain in bacteria

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   ITGB1 (ITGB1 Human)
   

   Tags

   MBP + uncleavable His10

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCVpic2neo-hTERT-FF2

   Plasmid

   #164910

   Purpose

   Expresses hTERT cDNA and FF2 shRNA in mammalian cells

   Depositor

   Ji Luo

   Article

   Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

   Inserts

   human telomerase reverse transcriptase (TERT Human)
   Neuropeptide FF receptor 2 (NPFFR2 Human)
   

   Use

   Retroviral

   Tags

   HA tag

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPBpuro-mNG-eDHFR(69K6)-Tiam1

   Plasmid

   #172102

   Purpose

   Mammalian expression of Tiam1(DH-PH) fused to mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   mNeonGreen-eDHFR(69K6)-Tiam1(DH-PH) (TIAM1 Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Topo_SpCas9.tracr_LbCas12a.DR

   Plasmid

   #155049

   Purpose

   TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)
   

   Use

   CRISPR

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEN_TGmiR_Gb2-K

   Plasmid

   #25766

   Purpose

   Entry vector withTRE promoter driving mouse G beta 2 miR30-based shRNA with GFP coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   Gb2 miR-shRNA (Gnb2 Mouse)
   

   Use

   Entry vector

   Tags

   GFP

   Available Since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. LK4-Ets2

   Plasmid

   #44606

   Depositor

   Robert Oshima

   Article

   Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

   Insert

   Ets2 (Ets2 Mouse)
   

   Expression

   Mammalian

   Promoter

   human Beta-Actin

   Available Since

   May 13, 2013

   Availability

   Academic Institutions and Nonprofits only

9. KN801: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-VPR

   Plasmid

   #121829

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   x-dCas9(3.7)/VPR (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. KN901: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-KRAB

    Plasmid

    #121830

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-dCas9(3.7)/KRAB (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. KO001: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-p300core

    Plasmid

    #121832

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-dCas9(3.7)/p300core (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223 LC3A G120

    Plasmid

    #123071

    Purpose

    Gateway entry clone encoding human MAP1LC3A G120

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Deleted amino acid 121. Stop codon after G120.

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. hEBI3 pEF6-Flag (MJS_003)

    Plasmid

    #72491

    Purpose

    Expression of human EBI3 in mammalian cells (Flag tag)

    Depositor

    Matthew Sweet

    Article

    Ramnath et al Immunol Cell Biol. 2015 Oct;93(9):771-9.

    Insert

    EBI3 (EBI3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ntng2.a-Fc-His

    Plasmid

    #72120

    Purpose

    Expresses the extracellular region of the Netrin G2, isoform a protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ntng2.a (Ntng2 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ntng1.b-Fc-His

    Plasmid

    #72111

    Purpose

    Expresses the extracellular region of the Netrin G1, isoform b protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ntng1.b (Ntng1 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBW2677_pCAG-PV1-FlpO-N396-L1-nMag-NLS-BGHpA

    Plasmid

    #108831

    Purpose

    Expresses N-terminus to aa396 of Flp recombinase fused to nMag CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment N-terminus to aa396
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    nMag,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ncam1_1.0.1-Fc-His

    Plasmid

    #72087

    Purpose

    Expresses the extracellular region of the NCAM1, isoform 1.0.1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ncam1_1.0.1 Mouse (Ncam1 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pInducer20 CARD8-HA PAM S297A

    Plasmid

    #169983

    Purpose

    Tet/Dox-inducible mammalian expression of CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PAM, S297A

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Gal4-entry

    Plasmid

    #128014

    Purpose

    Vector containing the Gal4-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancer

    Depositor

    Julius Brennecke

    Article

    Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

    Insert

    Gal4-DBD-3xFlag
    

    Use

    Gal4 entry vector

    Tags

    Gal4-DBD-3xFlag

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5FRT/TO PP1gamma-2*Strep

    Plasmid

    #113506

    Purpose

    Expresses dual Strep-tagged PP1gamma in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    PP1gamma (PPP1CC Human)
    

    Tags

    Strep-Strep

    Expression

    Mammalian

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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