We narrowed to 9,591 results for: Pol
-
Plasmid#123812PurposeEncodes rabbit beta-globin polyadenylation signal as a Type 4 part to be used in the MTK systemDepositorInsertrglb pA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JAK3 (JAK3A-c023)
Plasmid#98229PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertJAK3A (JAK3 Human)
UseBaculovirusTagsHis-6-TEVMutationpfam00069:Pkinase 822-1091PromoterpolyhedrinAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDB007ns2a
Plasmid#99214PurposeAccessory plasmid for PACE of PylRS variants; negative selectionDepositorInsertPpsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
Mutationamber codons at positions 12 and 203 of T7 RNA po…PromoterPpsp, PproK, and PtetAvailable SinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCbeta EV (PKA catalytic subunit Cbeta)
Plasmid#15311DepositorInsertPRKACB (Prkacb Mouse)
ExpressionMammalianAvailable SinceJuly 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDB023f1
Plasmid#99218PurposeComplementary plasmid for aaRS PACE; amber codons in T7 RNAPDepositorInsertPpsp [SD4] T7RNAP(S12*,S203*)
ExpressionBacterialMutationamber codons at positions 12 and 203 of T7 RNA po…PromoterPpspAvailable SinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
ERAP1
Plasmid#39174PurposeBaculovirus expression for structure determination; may not be full ORFDepositorAvailable SinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
LBJJ382
Plasmid#117518PurposeAlso known as LBJJ429. PCR template for sgRNA-EF with polyT, 67bp or 192bp terminatorDepositorInsertBpiI:ACTA:pU6-26:sgRNA-EF:t192bp:TTAC:BpiI
UseCRISPRExpressionPlantPromoterAtU6-26Available SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
hPDI1-18-508_C400S-pFLAG-CMV1
Plasmid#31384DepositorAvailable SinceMay 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOCC82
Plasmid#118862Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for untagged recombinant proteinDepositorInsertNcoI-NotI-ccdB-AscI-stop-HindIII cassette
ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-Histag_C
Plasmid#127035PurposeBasic Part C- C-terminus; polyhistidine tagDepositorInsertPart His6_C
UseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRset-OppA
Plasmid#207869PurposeBacterial expression of poly-his-tagged L. lactic OppADepositorInsertOppA
Tags6xHisExpressionBacterialPromoterT7Available SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-eSpCAS9-NG-pA
Plasmid#131466PurposeExpresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized eSpCas9-NG
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianAvailable SinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15bLCFPT7
Plasmid#53545PurposeActivation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistantDepositorInsertT7 RNAP
ExpressionBacterialAvailable SinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
MXS_bGpA
Plasmid#62424PurposeMXS_chaining vector with bGpADepositorInsertbeta-Globin poly A
UseSynthetic BiologyAvailable SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4092709
Plasmid#153859PurposeBacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTRDepositorInsertfragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionBacterialMutationNearly native encoding;handful SNPs as watermarks…PromoterPromoter | t7 consensus from NEB, G is +1Available SinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBACgus N-His/GST/LRRK2 1867-2186
Plasmid#40403DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsGST-Tev and HisTevMutationWT aa1867-2186PromoterpolHAvailable SinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-T3-SpCAS9-NG-pA
Plasmid#131465PurposeExpresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized SpCas9-NG
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianAvailable SinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
HOXA9-MSCV-full-GFP
Plasmid#20977DepositorInsertHOXA9 (Hoxa9 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationFull length cDNA with ~1.1kb 3'UTR with 2 pu…Available SinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
hTspan12(1-252)-6xHis-3C-sGFP11_pVL1393
Plasmid#216382PurposeBaculovirus transfer vector to express human Tspan12 ∆C (residues 1-252) fused to split GFP s11DepositorInsertTetraspanin-12 (TSPAN12 Human)
UseBaculovirusTags6xHis-3C-splitGFP11ExpressionInsectPromoterPolyhedrinAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB_init_Sb92
Plasmid#159424PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb92DepositorInsertSb92
Tagsc-Myc and poly-HisExpressionBacterialAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only