We narrowed to 26,999 results for: GFP

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pAM-AAV-mSncg-EGFP

Plasmid

#153163

Purpose

Mouse gamma-synuclein (mSncg) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

mSncg

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-PERK(LD)-EGFP-HOTag3

Plasmid

#225678

Purpose

Used to generate stable cell lines that express PERK(LD)-EGFP-HOTag3 and to detect the unfolded proteins in the ER lumen.

Depositor

Likun Wang

Article

Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-GFP-LC3-RFP

Plasmid

#84573

Purpose

Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP and mRFP1

Expression

Mammalian

Available Since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

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Venus-30aa-Noxa-pEGFP-C1

Plasmid

#166762

Purpose

To increase the length of the linker between Venus and Noxa proteins

Depositor

David Andrews

Insert

Noxa (PMAIP1 Human)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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PB-CMV-MCS-Puro-EGFP

Plasmid

#219795

Purpose

Mammalian expression plasmids using piggyBac Transposon System

Depositor

Keiichi Nakayama

Type

Empty backbone

Use

Piggybac transposon

Expression

Mammalian

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-mEGFP

Plasmid

#175293

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with mEGFP

Tags

mEGFP

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Str-Ii_VSVG-SBP-EGFP

Plasmid

#65300

Purpose

synchronize trafficking of VSVG from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

VSV-G

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pS34.EF1α<GFP-2A-Cas9-RC

Plasmid

#207404

Purpose

Cas9-RC and GFP from an EF1α promoter. High-performance knockin through DSB repair by homology-directed recombination (HDR) or homology-mediated end joining (HMEJ). [Lab plasmid ID: N213]

Depositor

Alexandros Poulopoulos

Article

Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

Insert

Cas9-RC

Use

CRISPR and Synthetic Biology

Tags

GFP-2A

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FlipCherry-T2A-GFP

Plasmid

#124436

Purpose

Expresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipCherry-T2A-GFP

Expression

Mammalian

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-EGFP-AID-BubR1

Plasmid

#47330

Purpose

for plasmid integration using the Flp-In system. AID at N-terminal

Depositor

Don Cleveland

Article

Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

Insert

BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)

Tags

AID and GFP

Expression

Mammalian

Mutation

siRNA resistant

Available Since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-HDR-mEGFP-Actin

Plasmid

#119870

Purpose

AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse Beta Actin

Depositor

Ryohei Yasuda

Article

Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

Insert

beta Actin (Actb Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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SacI ML GFP Strand 11 Long

Plasmid

#164120

Purpose

Detect the long Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

Depositor

Marisa Brini, Tito Calì

Article

Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

Insert

SacI ML GFP Strand 11 Long

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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SacI ML GFP Strand 11 Short

Plasmid

#164121

Purpose

Detect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

Depositor

Marisa Brini, Tito Calì

Article

Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

Insert

SacI ML GFP Strand 11 Short

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-mDlx-GFP-Fishell-1

Plasmid

#83900

Purpose

GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

eGFP

Use

AAV

Promoter

mDlx

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-SpCas9-GFP-U6-gRNA

Plasmid

#79144

Purpose

All-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSC

Depositor

Jizhong Zou

Article

Stoehr et al JCI Insight. 2019 May 21;5. pii: 128496.

Insert

SpCas9

Use

CRISPR

Tags

2A-GFP

Expression

Mammalian

Promoter

CAG

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-FAM134B

Plasmid

#109026

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

FAM134B (RETREG1 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

EIF-1a promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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TetOn-mCherry-eGFP-RAMP4

Plasmid

#109014

Purpose

Tet-On construct to induce expression of tandem eGFP-mCherry tagged RAMP4 (ER marker)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

RAMP4 (SERP1 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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EFSp-GFP-YAP (S94A)

Plasmid

#174169

Purpose

Lentiviral vector to constitutively express TEAD-binding mutant YAP under control of the EFS promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

3x FLAG tag

Expression

Mammalian

Mutation

Serine 94 mutated to Alanine

Promoter

EFS

Available Since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCW57.1 N-term GFP tTA

Plasmid

#107551

Purpose

All-in-one dox-repressible expression of cDNA with optional N-term GFP tag.

Depositor

David Sabatini

Type

Empty backbone

Use

Lentiviral

Tags

GFP (optional)

Expression

Mammalian

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pZ M2rtTA_CAGG TetON-Sox10 with GFP

Plasmid

#115241

Purpose

RMCE donor vector for inducible expression of SOX10 and GFP and constitutive expression of m2rtTA (generated from plasmid #112668)

Depositor

Catherine Verfaillie

Article

Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

Inserts

SOX10 (SOX10 Human)
eGFP

Tags

GFP (separate polypeptide)

Expression

Mammalian

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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