We narrowed to 13,360 results for: Mpl;

1. pTET GFP10-FRB/FKBP-GFP11

   Plasmid

   #182497

   Purpose

   Tripartite split-GFP. E. coli vector. Inducible coexpression of GFP10-FRB and FKBP-GFP11 coiled coils

   Depositor

   Stéphanie Cabantous

   Article

   Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

   Inserts

   FRB
   FKBP (Fkbp1a Mouse, Synthetic)
   

   Tags

   split GFP10 tripartite and split GFP11 tripartite

   Expression

   Bacterial

   Promoter

   TET

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. C1QBP-bio-His

   Plasmid

   #53330

   Purpose

   Expresses full-length Complement component 1 Q subcomponent-binding protein ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   C1QBP (C1QBP Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. YAP1-V5 in pLX304

   Plasmid

   #42555

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-N-Puro-mStayGold-LMNB1

   Plasmid

   #227329

   Purpose

   Donor template for Puro-2A-mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Puro-2A-mStayGold (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBridge-tyrosinase.σ2

   Plasmid

   #197415

   Purpose

   Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Inserts

   tyrosinase cytosolic tail
   AP-2 σ2
   

   Tags

   GAL4-DNA binding domain fragment, HA tag, and nuc…

   Expression

   Yeast

   Mutation

   encodes R42G substitution, contains silent substi…

   Promoter

   ADH1 and MET25

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-C2-MVP

   Plasmid

   #204543

   Purpose

   Expresses EGFP-tagged MVP in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

   Insert

   MVP (MVP Human)
   

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-T/O-Ds1-mCherry

   Plasmid

   #112855

   Purpose

   Dox inducible expression in mammalian cells of Ds1 protein fused to mCherry fluorescent protein

   Depositor

   David Sprinzak

   Article

   Loza et al Elife. 2017 Aug 18;6. pii: 24820. doi: 1

   Insert

   DCHS1 (DCHS1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   doxycycline inducible promoter

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-N-Blast-dTAG-LMNB1

   Plasmid

   #207776

   Purpose

   Donor template for Blast-2A-dTAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Blast-dTAG Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pD649-HAsp-SIGLEC8-COMP5AP-AviTag-9xHis

   Plasmid

   #157356

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SIGLEC8 (SIGLEC8 Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pD649-HAsp-SIGLEC15-COMP5AP-AviTag-9xHis

    Plasmid

    #157262

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SIGLEC15 (SIGLEC15 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTT3-SP-6xHis-NTRK1(FL)-FLAG

    Plasmid

    #157620

    Purpose

    Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    NTRK1 (NTRK1 Human)
    

    Tags

    6xHis and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX304 ERG

    Plasmid

    #118625

    Purpose

    Overexpresses ERG protein

    Depositor

    William Hahn

    Article

    Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

    Insert

    ERG (33-479) (ERG Human)
    

    Use

    Lentiviral

    Tags

    None and none

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. MBX

    Plasmid

    #64122

    Purpose

    a modified episomal (EBNA1/OriP) vector expressing human BCL2L1 (BCL-xL) gene

    Depositor

    Linzhao Cheng

    Article

    Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. OPEN Beta2-microglobulin

    Plasmid

    #215657

    Purpose

    E. coli expression of human beta-2-microglobulin containing a cysteine mutation for disulfide linkage to mutant HLA.

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN Beta2-microglobulin (B2M Human)
    

    Expression

    Bacterial

    Mutation

    Histidine 31 to Cysteine

    Promoter

    T7

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Pancortin scFv [K96/7]

    Plasmid

    #190497

    Purpose

    Mammalian Expression of Pancortin scFV. Derived from hybridoma K96/7.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Pancortin (Homo sapiens) recombinant scFV (OLFM1 Mouse)
    

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Brg1 Del2 pET28a

    Plasmid

    #122116

    Purpose

    Expression Brg1 amino acids 1-837 in bacteria

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brg1 (SMARCA4 Human)
    

    Tags

    6XHis and His-T7

    Expression

    Bacterial

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Brg1 Del3 pET28a

    Plasmid

    #122117

    Purpose

    Expression Brg1 amino acids 836-1446 in bacteria

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brg1 (SMARCA4 Human)
    

    Tags

    His-T7

    Expression

    Bacterial

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. qTAG-N-Blast-mScarlet-ACTB

    Plasmid

    #207753

    Purpose

    Donor template for Blast-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ACTB Homology Arms flanking a Blast-mScarlet Cassette (ACTB Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Dec. 1, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pBiex-1 AC30_eGFP

    Plasmid

    #82715

    Purpose

    Expresses a GFP-tagged recombinant adenylyl cyclase.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

    Insert

    Adenylyl Cyclase V/II (ADCY2, ADCY5 Human)
    

    Tags

    FLAG purification tag and eGFP

    Expression

    Bacterial and Insect

    Promoter

    T7 Promoter

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. qTAG-N-Puro-moxGFP-GOLGA2

    Plasmid

    #207792

    Purpose

    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the GOLGA2 locus for Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 Addgene #207791

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    GOLGA2 Homology Arms flanking a Puro-moxGFP Cassette (GOLGA2 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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