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pML364_PSMB7-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227922

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

PSMB7 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML374_CLTA-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227893

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

CLTA homology arms with mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML307_SEC61B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227894

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

SEC61B homology arms with mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML383_CCDC47-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227896

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

CCDC47 homology arms with mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML384_NCLN-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227897

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

NCLN homology arms with mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML318_SEC23A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227903

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

SEC23A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SFFV-HIF1A-Brd 190

Plasmid

#219149

Purpose

Transcription factor HIF1A with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

HIF1A (HIF1A Human)

Use

Lentiviral

Mutation

Codon optimized

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-RBPJ-Brd 204

Plasmid

#219158

Purpose

Transcription factor RBPJ with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

RBPJ (RBPJ Human)

Use

Lentiviral

Mutation

Codon optimized

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-EZH2-Brd 228

Plasmid

#219171

Purpose

Transcription factor EZH2 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

EZH2 (EZH2 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-RUNX1-Brd 75

Plasmid

#219053

Purpose

Transcription factor RUNX1 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-TERF1-Brd 381

Plasmid

#219306

Purpose

Transcription factor TERF1 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

TERF1 (TERF1 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-EPAS1-Brd 375

Plasmid

#219300

Purpose

Transcription factor EPAS1 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

EPAS1 (EPAS1 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-BCL11A-Brd 469

Plasmid

#219370

Purpose

Transcription factor BCL11A with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

BCL11A (BCL11A Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM1-TSC22D2-Cterm-C-FLAG

Plasmid

#222685

Purpose

overexpressing C-terminus truncation of TSC22D2 with C-terminal FLAG

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D2 (TSC22D2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

TSC22D2 C-terminus truncation

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pYX-N-Puro-mNeon-TSC22D1

Plasmid

#222667

Purpose

endogenously tag TSC22D1 with N-terminal mNeon

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D1 (TSC22D1 Human)

Tags

mNeon

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pYX-N-Blast-miniTurbo-TSC22D2

Plasmid

#222669

Purpose

endogenously tag TSC22D2 with N-terminal miniTurbo

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D2 (TSC22D2 Human)

Tags

miniTurbo

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM1-NRBP1-FL-N-FLAG

Plasmid

#222675

Purpose

overexpressing full length NRBP1 with N-terminal FLAG

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

NRBP1 (NRBP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM1-NRBP1-Nterm-N-FLAG

Plasmid

#222676

Purpose

overexpressing N-terminus truncation of NRBP1 with N-terminal FLAG

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

NRBP1 (NRBP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

NRBP1 N-terminus truncation

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM1-NRBP1-Nterm-C-FLAG

Plasmid

#222677

Purpose

overexpressing N-terminus truncation of NRBP1 with C-terminal FLAG

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

NRBP1 (NRBP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

NRBP1 N-terminus truncation

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM1-NRBP1-Cterm-N-FLAG

Plasmid

#222678

Purpose

overexpressing C-terminus truncation of NRBP1 with N-terminal FLAG

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

NRBP1 (NRBP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

NRBP1 C-terminus truncation

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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