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mApple-M1 SpastinE442Q

Plasmid

#134464

Purpose

M1 SpastinE442Q expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

Glu to Gln at amino acid 442

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-mCherry-SPASTIN

Plasmid

#180647

Purpose

Lentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Starts at M87, has siRNA resistance to GAACAGUGUG…

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pQTEV-HSPB8

Plasmid

#34710

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

heat shock 22 kDa protein 8 (HSPB8 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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mApple-M1 SpastinK388R

Plasmid

#134463

Purpose

M1 SpastinK388R expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

Lys to Arg at amino acid 388

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pRK-FLAG-TRAF4

Plasmid

#58316

Purpose

FLAG-tagged mouse TRAF4

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pCA528 SPASTIN , residues 108-200

Plasmid

#180628

Purpose

Bacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 108-200

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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HA, EGFP Spastin M87 with exon 4, N386C

Plasmid

#167543

Purpose

For doxycycline inducible mammalian expression of EGFP tagged Spastin M87 with exon 4, with N386C mutation.

Depositor

Tarun Kapoor

Article

Cupido et al Nat Chem Biol. 2019 May;15(5):444-452. d

Insert

Spastin M87 with exon 4 (SPAST Human)

Use

Tags

EGFP and HA tag

Expression

Mammalian

Mutation

N386C

Promoter

CMV

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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mApple-M11-92

Plasmid

#134466

Purpose

M11-92 expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin Amino Acids 1-92 (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

M1 Spastin Amino Acids 1-92

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCA528 SPASTIN , residues 112-196

Plasmid

#180627

Purpose

Bacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 112-196

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-PP-Spastin(87-616)DeltaExon4

Plasmid

#128794

Purpose

Bacteiral expression of Spastin M87 varient without exon 4

Depositor

Chris Hill

Article

Han et al J Biol Chem. 2019 Nov 25. pii: AC119.009

Insert

Spastin (SPAST Human)

Use

`

Tags

GST

Expression

Bacterial

Mutation

residues 87-616 without exon 4.

Promoter

T7

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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