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pIntegrase_4

Plasmid

#60576

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_4

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_2

Plasmid

#60574

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_2

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_7

Plasmid

#60578

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_7

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_5

Plasmid

#60577

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_5

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_12

Plasmid

#60583

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_12

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_13

Plasmid

#60584

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_13

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_11

Plasmid

#60582

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_11

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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AAVS1-TALEN-L

Plasmid

#59025

Purpose

TALEN designed to target the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

AAVS1-TALEN-L

Use

AAV and TALEN

Tags

3xFlag and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only

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AAVS1-TALEN-R

Plasmid

#59026

Purpose

TALEN designed to target the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

AAVS1-TALEN-R

Use

AAV and TALEN

Tags

3xFlag and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-hPREP

Plasmid

#179387

Purpose

Express human prolyl oligopeptidase (tagged) in bacterial cells

Depositor

Christian Gruber

Article

Retzl et al J Nat Prod. 2020 Nov 25;83(11):3305-3314

Insert

human prolyl oligopeptidase (PREP Human)

Tags

GST

Expression

Bacterial

Promoter

TAC

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-blast

Plasmid

#26655

Purpose

3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses blasticidin for selection.

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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mRFP-Lact-C2

Plasmid

#74061

Purpose

Fluorescent biosensor for phosphatidylserine.

Depositor

Sergio Grinstein

Article

Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

Insert

Lactadherin-C2 (MFGE8 Bovine)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

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pJRH-1362 scFv entry plasmid

Plasmid

#201912

Purpose

Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pJRH-1187 VSVGmut

Plasmid

#201913

Purpose

Expresses a VSV-G variant (K47Q R354A) defective for LDL-R receptor binding (CAG promoter)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

VSV-G (K47Q, R354A)

Expression

Mammalian

Mutation

K47Q, R354A

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-GFP-Sec61β

Plasmid

#186597

Purpose

Third generation lentiviral vector expressing GFP-Sec61β

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Sec61β (SEC61B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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UbG76V-eGFP-V5His_20aa

Plasmid

#23970

Depositor

Nico Dantuma

Article

Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

Insert

Ub-G76V-eGFP-V5HIS_20aa

Tags

eGFP

Expression

Mammalian

Mutation

G76V mutation in Ubiquitin. Ub fused to N-termin…

Available Since

March 22, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3 CDK7 HA (P#633)

Plasmid

#14647

Depositor

Matija Peterlin

Article

Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

Insert

Cdk7 (CDK7 Human)

Tags

HA

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MDM2 WT

Plasmid

#16233

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pLSU4cg10

Plasmid

#126078

Purpose

Plant transformation vector for stable expression of plastid targeted GFP1-10 in plant cells

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

longCaMV35S

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-MCN-10His-mStayGold (E138D)

Plasmid

#211361

Purpose

Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Article

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

mStayGold

Tags

10xHis-tag

Expression

Bacterial

Mutation

E138D

Promoter

T7

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
DYSF-3HA

Plasmid

#29767

Depositor

Steven Vogel

Article

Covian-Nares et al J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-

Insert

DYSF (DYSF Human)

Tags

3xHA

Expression

Bacterial and Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF1-alpha-V

Plasmid

#27290

Depositor

Timothy Springer

Article

Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

Insert

integrin alpha V (ITGAV Human)

Expression

Mammalian

Mutation

Isoform 2

Available Since

March 8, 2011

Availability

Academic Institutions and Nonprofits only

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pLX301-ZIM3-KRAB-PYL1

Plasmid

#154761

Purpose

Expresses ZIM3 KRAB domain fused to PYL1

Depositor

Mikko Taipale

Article

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Promoter

CMV

Available Since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCSC-SP-PW-NepX (aka: pBOB-NEPX)

Plasmid

#12340

Depositor

Inder Verma

Article

Marr et al J Mol Neurosci. 2004 . 22(1-2):5-11.

Insert

NEP-X (MME Human)

Use

Lentiviral

Expression

Mammalian

Mutation

E585V (inactive mutant)

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
Klc1-GFP

Plasmid

#186617

Purpose

Encodes Kinesin Light Chain 1 fused to GFP

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Light Chain 1 (KLC1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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LRG (Lenti_sgRNA_EFS_GFP)

Plasmid

#65656

Purpose

Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

Depositor

Christopher Vakoc

Article

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter-driven sgRNA expression and EFS promo…

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-2A-Puro (PX459)

Plasmid

#48139

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
pCS2+-H2B-pr-mEosFP

Plasmid

#141101

Purpose

H2B fused to pr-mEosFP for chromatin visualization and primed conversion

Depositor

Periklis Pantazis

Article

Welling et al Bio Protoc. 2020 Jun 5;10(11):e3645. doi

Insert

Histone 2B

Tags

pr-mEosFP

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-GFP-IRES-CRE

Plasmid

#48201

Purpose

retroviral vector encoding for GFP and Cre-recombinase

Depositor

Fred Gage

Article

Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

Inserts

eGFP
Cre

Use

Retroviral

Expression

Mammalian

Promoter

CAG and linked by IRES

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSC115

Plasmid

#216356

Purpose

xNup93-Nb2t Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup93-Nb2t

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV BAF170-Flag

Plasmid

#19142

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2 (SMARCC2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
SIRT1 Flag

Plasmid

#13812

Purpose

Mammalian expression of human SIRT1 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT1 (SIRT1 Human)

Tags

flag

Expression

Mammalian

Mutation

N-terminus truncated

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV_JAK3_IRES_BFP

Plasmid

#206291

Purpose

Expresses the wild type form of human JAK3.

Depositor

Charles de Bock

Article

Omari et al Heliyon. 2023 Nov 8;9(11):e22085. doi: 1

Insert

JAK3 (JAK3 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330A-FBL/PITCh

Plasmid

#63671

Purpose

Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only
pCS_FLPER(T2)

Plasmid

#31303

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

FLP-ER ligand binding domain (ESR1 Budding Yeast, Human)

Expression

Mammalian

Mutation

full length FLP linked to ligand binding domain o…

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCas9n(D10A)-Blast

Plasmid

#63593

Purpose

Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

Depositor

Feng Zhang

Article

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Inserts

Cas9
Blasticidin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A, nickase mutant

Promoter

EFS-NS

Available Since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSIIhyg-rtTA

Plasmid

#139480

Purpose

Expresses rtTA, Tet-ON reverse tetracycline-controlled trans-activator in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

rtTA, Tet-ON reverse transactivator

Use

Lentiviral

Expression

Mammalian

Promoter

Human EF1a promoter

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-HA-rat Nrxn3beta AS4(-)

Plasmid

#58269

Purpose

Expression plasmid for HA-tagged rat Neurexin 3beta, lacking the insert at alternatively spliced segment 4 (AS4-)

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn3beta AS4(-) (Nrxn3 Rat)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-CBLB

Plasmid

#192082

Purpose

Mammalian expression of human CBLB with FLAG tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

CBLB (CBLB Human)

Tags

FLAG

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1-Puro Xlone-eGFP

Plasmid

#136936

Purpose

donor plasmid for inducible transgene expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

all-in-one tet-on system

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3GS

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IZ-ANXA1-mRuby3

Plasmid

#184888

Purpose

Stable expression of ANXA1-mRuby3 (Annexin A1-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA1 (ANXA1 Human)

Tags

mRuby3

Expression

Mammalian

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-21a(+)-AK1-His

Plasmid

#118977

Purpose

Bacterial expression plasmid for Adenylate kinase isoenzyme 1

Depositor

Sebastian Maerkl

Article

Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

Insert

adenylate kinase isoenzyme 1 AK1 (AK1 Chicken)

Tags

6x-His

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pKAR2-Br512_Mut235

Plasmid

#179278

Purpose

Expresses Br512_Mut235 polymerase in E.coli

Depositor

Andrew Ellington

Article

Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

Insert

Engineered Bst DNA Polymerase I Large Fragment

Tags

8x His tag; Villin Headpiece 47 amino acid (HP47)…

Expression

Bacterial

Promoter

T7

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
RP1B-PP1alpha

Plasmid

#51768

Purpose

Expresses PP1 alpha residues 7-330

Depositor

Wolfgang Peti

Article

Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

Insert

pp1 alpha

Tags

His, TEV protease site, and Thio-6

Expression

Bacterial

Mutation

Contains PP1 alpha residues 7-330

Promoter

T7-lac

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
pBbB8k-csg-NbGFP

Plasmid

#166858

Purpose

Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.

Depositor

Neel Joshi

Article

Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469

Insert

csgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5-Flag-RhoA wt

Plasmid

#11750

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

RhoA (RHOA Human)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pB-Neo-mtkeima

Plasmid

#210338

Purpose

Overexpression of mtkeima

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

mtkeima

Expression

Mammalian

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-CD68 promoter/enhancer

Plasmid

#34837

Depositor

Peter Murray

Article

Lang et al J Immunol. 2002 Apr 1;168(7):3402-11.

Insert

CD68 promoter/enhancer -680/+140 (CD68 Human)

Expression

Mammalian

Available Since

Jan. 18, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSP1E3

Plasmid

#20064

Depositor

Stephen Sligar

Article

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E3 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeats of he…

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Bak

Plasmid

#32564

Depositor

Richard Youle

Article

Nechushtan et al J Cell Biol. 2001 Jun 11. 153(6):1265-76

Insert

human Bak (BAK1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 7,325 results for: 11