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MLV Gag-RFP

Plasmid

#1814

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

Gag (gag MuLV)

Use

Tags

RFP

Expression

Mammalian

Mutation

Promoter

Available since

May 19, 2005

Availability

Academic Institutions and Nonprofits only

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pVITRO1-TagRFP-NLS

Plasmid

#184446

Purpose

Nucleus-targeted TagRFP expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

TagRFP-3xNLS

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD/HisD-rsTagRFP

Plasmid

#31927

Depositor

Vladislav Verkhusha

Article

Subach et al Chem Biol. 2010 Jul 30;17(7):745-55.

Insert

rsTagRFP

Use

Tags

His

Expression

Bacterial

Mutation

F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…

Promoter

araB

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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gRNA-MS2x2-TagRFP657

Plasmid

#184441

Purpose

sgRNA with MS2 aptamer, TagRFP657 as selection marker

Depositor

Rhys Allan

Article

Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

Insert

sgRNA-MS2x2, TagRFP657

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-hSyn-RFP

Plasmid

#22909

Depositor

Edward Callaway

Article

Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

Has Service

Cloning Grade DNA

Insert

human synapsin I promoter driving DsRedExpress (SYN1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2010

Availability

Academic Institutions and Nonprofits only

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pLEM415-ldhL-mRFP1

Plasmid

#99842

Purpose

Expression of RFP in Lactobacillus

Depositor

Sujin Bao

Article

Bao et al PLoS One. 2013;8(3):e60007. doi: 10.1371

Insert

monomeric red fluorescent protein

Use

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

ldhl

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLL7.0: tgRFPt-SSPB WT

Plasmid

#60415

Purpose

Mammalian Expression of tgRFPt-Nano

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

tgRFPt-SSPB

Use

Cre/Lox and Lentiviral

Tags

TagRFPt

Expression

Mammalian

Mutation

Y11K and A15E

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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AICSDP-28:TUBA1B-mTagRFP-T

Plasmid

#101785

Purpose

Homology arms and mTagRFPT-linker sequence for N-terminus (second exon) tagging of human TUBA1B

Depositor

Allen Institute for Cell Science

Insert

TUBA1B Homology Arms with mTagRFPT-linker (TUBA1B Human)

Use

CRISPR; Donor template

Tags

mTagRFPT-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

Oct. 31, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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LentiGuide-TagRFP-2A-BSD

Plasmid

#167930

Purpose

Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLViP-LifeAct-iRFP670

Plasmid

#229699

Purpose

Lentiviral expression of LifeAct-iRFP670 in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

Synthetic construct LifeAct-mTurquoise gene

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LAMP1-mRFP-FLAG

Plasmid

#34611

Purpose

3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tag

Depositor

David Sabatini

Article

Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

2xFlag and mRFP

Expression

Mammalian

Mutation

Promoter

Available since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mito-TagRFP-T

Plasmid

#174543

Purpose

labels mitochondria with TagRFP-T

Depositor

Minna Roh-Johnson

Article

Kidwell et al Elife. 2023 Mar 6;12:e85494. doi: 10.755

Insert

MTS-tagRFP-T

Use

Lentiviral

Tags

tagRFP-T

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDV24_AAV2_DART-VADAR_iRFP720-sensor

Plasmid

#200411

Purpose

AAV vector encoding a DART-VADAR sensor for iRFP720 mRNA

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

DART VADAR sensor

Use

AAV and Synthetic Biology

Tags

MCP-ADAR2dd(E488Q)-NES, TagBFP, and mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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piRFP-FRB-Giantin

Plasmid

#139313

Purpose

Golgi-resident FRB for chemically induced dimerization

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

iRFP:MTOR(2021-2113):[GGSA]2:GOLGB1(3097-3226)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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RFP-KIF1B beta-FL

Plasmid

#79111

Purpose

Mammalian expression of RFP-KIF1B beta-FL

Depositor

Susanne Schlisio

Article

Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

Insert

RFP-KIF1B beta full length (KIF1B Human)

Use

Tags

RFP

Expression

Mammalian

Mutation

exon 25 missing (natural splice varient)

Promoter

CMV

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_iSH2-pMag(3x)-iRFP

Plasmid

#67304

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

iSH2-pMag(3x)-iRFP (PIK3R1 Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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TAPBPL pmRFP-N2 (721)

Plasmid

#62000

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

TAPBPL (TAPBPL Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pCSC-NLS-RFP

Plasmid

#221813

Purpose

Lentiviral vector expressing red fluorescent protein (RFP) fused with nuclear localization sequence (NLS)

Depositor

Baojin Ding

Article

Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

Insert

NLS-mCherry

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-H2B-iRFP720

Plasmid

#128961

Purpose

Lentiviral plasmid for expression of far red iRFP720 nuclear marker

Depositor

Carlos Carmona-Fontaine

Insert

Human histone H2B (H2BC21 Human)

Use

Lentiviral

Tags

iRFP720

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pShuttle-CMV-iRFP

Plasmid

#31856

Purpose

a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

Depositor

Vladislav Verkhusha

Article

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Insert

iRFP

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

Promoter

CMV

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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