We narrowed to 165,214 results for: addgene

1. pWT015a

   Plasmid

   #96878

   Purpose

   GFP reporter

   Depositor

   David Liu

   Article

   Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

   Insert

   GFP
   

   Expression

   Mammalian

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-fDIO-JRGECO1a

   Plasmid

   #128317

   Purpose

   Expresses JRGECO1a under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

   Depositor

   Patricia Jensen

   Has Service

   AAV1

   Insert

   NES-JRGECO1a
   

   Use

   AAV

   Tags

   His Tag

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. PERK 6: PERK.9E10.pBABE

   Plasmid

   #21819

   Depositor

   David Ron

   Insert

   PERK (Eif2ak3 Mouse)
   

   Use

   Retroviral

   Tags

   Myc (9E10)

   Expression

   Mammalian

   Available Since

   March 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV V5-lamin B1

   Plasmid

   #175108

   Purpose

   Lentiviral expression of V5-tagged mouse Lmnb1

   Depositor

   Larry Gerace

   Insert

   Lmnb1 (Lmnb1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV-Jarid1c-IRES-GFP

   Plasmid

   #166009

   Purpose

   Retroviral biscistronic expression of Jarid1c and eGFP

   Depositor

   Manu Rangachari

   Insert

   Jarid1c (Kdm5c Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTF275 (pFA6a-TEV-6xGly-2xStrep-His3MX)

   Plasmid

   #44070

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   2xStrep tag, 6xGly linker, ADH1 transcription ter…

   Available Since

   April 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. meGFP-hTRAK1

   Plasmid

   #188664

   Purpose

   human TRAK1 with N-terminal meGFP tag

   Depositor

   Thomas Schwarz

   Insert

   OIP106 (TRAK1 Human)
   

   Tags

   meGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTF263 (pFA6a-TEV-6xGly-12xHis-His3MX)

   Plasmid

   #44069

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   12xHis tag, 6xGly linker, ADH1 transcription term…

   Available Since

   April 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. UHRF1

   Plasmid

   #28163

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   UHRF1 (UHRF1 Human)
   

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   tandem tudor domain

   Available Since

   Feb. 28, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pAAV-CMV-FLEX-SaCas9-U6-sgOprk1

    Plasmid

    #159903

    Purpose

    Mutagenesis of Oprk1

    Depositor

    Larry Zweifel

    Insert

    Oprk1 (Oprk1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-BE4max-P2A-EGFP (pRZ33)

    Plasmid

    #140083

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP

    Depositor

    Keith Joung

    Insert

    Human codon optimized pCAG BE4max with P2A-EGFP
    

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A (SpCas9)

    Promoter

    CAG

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. XLone-Puro SPI1 P2A mCherry

    Plasmid

    #140029

    Purpose

    Tunable and temporal expression control of SPI1 and mCherry

    Depositor

    Xiaoping Bao

    Insert

    SPI1 (SPI1 Human)
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-HA_human_BAP1

    Plasmid

    #158109

    Purpose

    Expresses tagged BAP1, neomycin selection

    Depositor

    Emanuela Felley-Bosco

    Insert

    BAP1 (BAP1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)

    Plasmid

    #176932

    Purpose

    Donor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hGEM(1-110)_mNG_hSLBP(18-108)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    PGK1

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. XLone-BSD SPI1 P2A mCherry

    Plasmid

    #140031

    Purpose

    Tunable and temporal expression control of SPI1 and mCherry

    Depositor

    Xiaoping Bao

    Insert

    SPI1 (SPI1 Human)
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV-mMDM2-IRES-GFP

    Plasmid

    #66954

    Purpose

    retroviral construct for expression of mouse Mdm2 cloned into the EcoRI site

    Depositor

    Martine Roussel

    Insert

    Mdm2 (Mdm2 Mouse)
    

    Use

    Retroviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CMV-FLEX-SaCas9-U6-sgNts

    Plasmid

    #159909

    Purpose

    Mutagenesis of Nts

    Depositor

    Larry Zweifel

    Insert

    Nts (Nts Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTF277 (pFA6a-TEV-6xGly-2xStrep-KanMX)

    Plasmid

    #44094

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    2xStrep tag, 6xGly linker, ADH1 transcription ter…

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CMV-FLEX-SaCas9-U6-sgVamp2

    Plasmid

    #159920

    Purpose

    Mutagenesis of Vamp2

    Depositor

    Larry Zweifel

    Insert

    Vamp2 (Vamp2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 4QQD

    Plasmid

    #61947

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    UHRF1:SDC130-H02:C39674 (UHRF1 Human)
    

    Tags

    6xHis and MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits