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Addgene

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We narrowed to 159,238 results for: addgene

Showing: 821 - 840 of 159238 results

  1. pTF276 (pFA6a-PSc-6xGly-2xStrep-His3MX)

    Plasmid
    #44064
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    2xStrep tag, 6xGly linker, ADH1 transcription ter…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44064-plasmid-map-sequence-id-61680

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  2. OTU04

    Plasmid
    #28157
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    OTU domain containing 7A (OTUD7A Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    UBA-like domain only
    Promoter
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. pPyCAG-Nanog(Y136A)-IP

    Plasmid
    #62995
    Purpose
    Expresses Mouse Nanog Y136A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Y136A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Tyrosine 136 (137 AA in Mouse Protein) to…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62995-plasmid-map-sequence-id-104597

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  4. p14-9VNA5

    Plasmid
    #50931
    Purpose
    Expresses human SRP14A5 and SRP9 proteins as a fusion protein in mammalian cells.
    Depositor
    Katharina Strub
    Insert
    SRP14 and SRP9 (SRP14, SRP9 Human)
    Use
    Tags
    VN tag and flag tag
    Expression
    Mammalian
    Mutation
    Amino acids 96-100 of SRP14 changed to alanine
    Promoter
    CMV
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pZC05 (pFA6a-TEV-6xGly-PrtA-KanMX)

    Plasmid
    #44096
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Expression
    Mutation
    Promoter
    Available since
    April 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    44096-plasmid-map-sequence-id-61688

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  6. pTFCP2.1.0-gDNA

    Plasmid
    #112404
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFCP2
    Depositor
    Kevin White
    Insert
    TFCP2 (TFCP2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112404-plasmid-map-sequence-id-230259

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  7. pZC10 (pFA6a-PSc-6xGly-V5-His3MX)

    Plasmid
    #44067
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, His3…
    Expression
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44067-plasmid-map-sequence-id-61693

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  8. pZC06 (pFA6a-PSc-6xGly-PrtA-KanMX)

    Plasmid
    #44090
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Expression
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44090-plasmid-map-sequence-id-61689

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  9. pZC14 (pFA6a-PSc-6xGly-V5-HphMX)

    Plasmid
    #44079
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44079-plasmid-map-sequence-id-61697

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  11. pZC07 (pFA6a-TEV-6xGly-PrtA-HphMX)

    Plasmid
    #44084
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Expression
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44084-plasmid-map-sequence-id-61690

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  12. pTF260 (pFA6a-PSc-6xGly-KanMX)

    Plasmid
    #44086
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44086-plasmid-map-sequence-id-61663

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  13. pPyCAG-Nanog(K137A)-IP

    Plasmid
    #62996
    Purpose
    Expresses Mouse Nanog K137A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog K137A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Lysine 137 (138 AA in Mouse Protein) to A…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62996-plasmid-map-sequence-id-104598

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  14. pPyCAG-Nanog(Y119A)-IP

    Plasmid
    #62991
    Purpose
    Expresses Mouse Nanog Y119A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Y119A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Tyrosine 119 (120 AA in Mouse Protein) to…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62991-plasmid-map-sequence-id-104440

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  15. pZC12 (pFA6a-PSc-6xGly-V5-KanMX)

    Plasmid
    #44091
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44091-plasmid-map-sequence-id-61695

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  16. pTF265 (pFA6a-PSc-6xGly-12xHis-His3MX)

    Plasmid
    #44063
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44063-plasmid-map-sequence-id-61668

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  17. TDRD5

    Plasmid
    #32869
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TDRD5 (TDRD5 Human)
    Use
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. pET15bHis6-pG-Tnp

    Plasmid
    #124884
    Purpose
    To transform BL21-Gold(DE3) and induced by IPTG for His-proteinG-Tnp (hyperactive mutant) fusion protein.
    Depositor
    Keji Zhao
    Insert
    protein G's C1D1C2D2C3 domains contained in the protein G gBLOCK.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    124884-plasmid-map-sequence-id-243752

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  19. pEN_TT DNM1L-V5

    Plasmid
    #220359
    Purpose
    Gateway entry vector for an inducible V5-tagged DNM1L
    Depositor
    Kevin Janes
    Insert
    DNM1L (DNM1L Human)
    Use
    Entry vector for gateway cloning
    Tags
    V5
    Expression
    Mutation
    Promoter
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220359-plasmid-map-sequence-id-447351

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  20. pAS_MmaPylT_EF1_NES-MmaPylRS WT

    Plasmid
    #174901
    Purpose
    expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG and NES
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174901-plasmid-map-sequence-id-348003

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Showing: 821 - 840 of 159238 results