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pDONR221-HUWE1_BH3-4E

Plasmid

#187143

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

V1976E/V1780E/L1983E/M1987E

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_delta169-189

Plasmid

#187134

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

delta169-189

Promoter

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_delta3896-3951

Plasmid

#187132

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

delta3896-3951

Promoter

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_I3969A/F3982A

Plasmid

#187128

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

I3969A/F3982A

Promoter

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_YH/GG_delta3291-3298

Plasmid

#187126

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

Y355G/H356G, delta3291-3298

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_delta3291-3298

Plasmid

#187124

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

delta3291-3298

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_YH/GG

Plasmid

#187122

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

Y355G/H356G

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-Sig10(DM)-Fc-IG

Plasmid

#183725

Purpose

Expresses the fusion protein containing the extracellular part of the human Siglec-10 R47Q A108V mutant and the Fc region of human IgG1, and GFP

Depositor

Takeshi Tsubata

Article

Alborzian Deh Sheikh et al J Autoimmun. 2021 Jan;116:102571. doi: 1

Insert

Siglec-10

Use

Retroviral

Tags

Fc of human IgG1

Expression

Mutation

R47Q and A108V

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Prom1 Del AA 6

Plasmid

#184024

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of amino acids 689 to 694.

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 SV8 Del AA 6 (Prom1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of amino acids 689 to 694

Promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Prom1 D-1

Plasmid

#184013

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of amino acids 685-706.

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 SV8(-Ex19a) D-1 (Prom1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of amino acids 685-706.

Promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40-A253FY208A_X

Plasmid

#147973

Purpose

Insect Expression of DmCaf40-A253FY208A

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-A253FY208A (Rcd-1 Fly)

Use

Tags

EGFP (A179V)

Expression

Insect

Mutation

Promoter

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCaf40-F65AC69Y_X

Plasmid

#147993

Purpose

Insect Expression of DmCaf40-F65AC69Y

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-F65AC69Y (Rcd-1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCaf40_25-291_W

Plasmid

#147866

Purpose

Insect Expression of DmCaf40_25-291

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40_25-291 (Rcd-1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pFL GST-UBXN2A

Plasmid

#169016

Purpose

Expresses GST-UBXN2A (human) in insect cells

Depositor

Hemmo Meyer

Article

Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

Insert

UBXN2A (UBXN2A Human)

Use

Tags

GST

Expression

Insect

Mutation

Promoter

polyhedrin promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3566 (pDEST15_Spt6 1223-1451)

Plasmid

#91814

Purpose

Bacterial expression of residues 1223-1451 from S. cerevisiae Spt6

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

SPT6 (SPT6 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

deleted residues 1-1222

Promoter

T7

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH1141 (pET151_Spt6 1247-1451)

Plasmid

#91815

Purpose

Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

SPT6 (SPT6 Budding Yeast)

Use

Tags

6xHis

Expression

Bacterial

Mutation

deleted residues 1-1246

Promoter

T7

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_DOK6_IRS

Plasmid

#109886

Purpose

Protein expression and purification of DOK6_IRS

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DOK6_IRS (DOK6 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_DOK3_IRS

Plasmid

#109885

Purpose

Protein expression and purification of DOK3_IRS

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DOK3_IRS (DOK3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_CAPN2_EF-hand

Plasmid

#109858

Purpose

Protein expression and purification of CAPN2_EF-hand

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

CAPN2_EF-hand (CAPN2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_DLG1_PDZ_1

Plasmid

#109998

Purpose

Protein expression and purification of DLG1_PDZ_1

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DLG1_PDZ_1 (DLG1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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