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pJK642
Plasmid#72391PurposeProduces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)DepositorInsertglutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
UseTagsHis6ExpressionBacterialMutationMutated tyrosine-397 to phenylalanine to block ad…PromoterT7Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
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PKM2 (3GQY)
Plasmid#25360PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPKM2_1 (PKM Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
PANK1
Plasmid#32871PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPANK1 (PANK1 Human)
UseTagsHis tag (with Thrombin cleavage)ExpressionBacterialMutationamino acids 231-597 onlyPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
UBE2H
Plasmid#25618PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertUBE2H (UBE2H Human)
UseTagsHisExpressionBacterialMutationContains amino acid residues 1-179PromoterAvailable sinceFeb. 2, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
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4P7A
Plasmid#61954PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMLH1:JMC01L-F09:C205690 (MLH1 Human)
UseTags6xHis and MHHHHHHSSGRENLYFQGExpressionBacterialMutationSee commentsPromoterT7Available sinceMarch 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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NIRF
Plasmid#25602PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertNIRF (UHRF2 Human)
UseTagsHisExpressionBacterialMutationContains Ring finger domainPromoterAvailable sinceAug. 30, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
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HOXC8 (murine) HIS-tag pET
Plasmid#8567DepositorInsertHOXC8 (Hoxc8 Mouse)
UseTagsHis and T7ExpressionBacterialMutationmakes protein by WesternPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
USP37
Plasmid#36898PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertUSP37 (USP37 Human)
UseTagsHis tag with thrombin cleavageExpressionBacterialMutationcontains amino acid residues 4-125 onlyPromoterAvailable sinceJune 21, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits