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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,322 results for: pET28a

Showing: 821 - 840 of 1322 results

  1. ppie.001.301.W257A

    Plasmid
    #137652
    Purpose
    Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.
    Depositor
    Melissa Jurica, Tara Davis
    Insert
    ppie (PPIE Human)
    Use
    Tags
    GST-(His)6-thrombin
    Expression
    Bacterial
    Mutation
    001-301-W257A
    Promoter
    Available Since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137652-plasmid-map-sequence-id-381379

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  2. 6PED

    Plasmid
    #162265
    Purpose
    Baculovirus expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    HEMK2 (N6AMT1 Human)
    Use
    Tags
    6xHis-Thrombin
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162265-plasmid-map-sequence-id-319828

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  3. UBH12 (3DB4)

    Plasmid
    #25171
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubh12.414.617:B9 (UHRF1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    contains residues 126-285
    Promoter
    Available Since
    April 4, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. pJK691

    Plasmid
    #72381
    Purpose
    Produces Escherichia coli BolA, N-terminal His6 tag (H6EcBolA)
    Depositor
    T. J. Kappock
    Insert
    stationary-phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC (bolA Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72381-plasmid-map-sequence-id-134006

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  5. pJK404

    Plasmid
    #72417
    Purpose
    Produces Acetobacter aceti 1023 Est12, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    acyl-CoA thioesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    additional N-terminal 15 residues MSETYGTGQKEQHC,…
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72417-plasmid-map-sequence-id-135656

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  6. CDY

    Plasmid
    #25507
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CDY (CDY2B Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    catalytic domain
    Promoter
    Available Since
    Sept. 10, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. pMCRW1

    Plasmid
    #122462
    Purpose
    Human NPM-ALK fusion protein gene in pET28a bacterial expression plasmid. Production of recombinant human NPM-ALK fusion protein in E. coli Bl21 Rosetta2 cells
    Depositor
    Richard C. Willson
    Article
    Kourentzi et al Sci Rep. 2020 Mar 19;10(1):5078. doi: 10
    Insert
    NPM-ALK fusion protein (ALK, NPM1 Human)
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac promoter
    Available Since
    May 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    122462-plasmid-map-sequence-id-243544

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  8. pJK642

    Plasmid
    #72391
    Purpose
    Produces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72391-plasmid-map-sequence-id-133988

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  9. COMTD1

    Plasmid
    #25484
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    COMTD1 (COMTD1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. HECTD1

    Plasmid
    #25183
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    hectd1.1273.1343 (HECTD1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 9, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. USP5

    Plasmid
    #25299
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP5.001.835 (USP5 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. PPIL2

    Plasmid
    #25601
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PPIL2 (PPIL2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 30, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. USP21 (3I3T)

    Plasmid
    #25147
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    usp21.209.563 (USP21 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. CDC34

    Plasmid
    #25598
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CDC34 (CDC34 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. SIRT6

    Plasmid
    #41564
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SIRT6 (SIRT6 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    aa 3-318
    Promoter
    T7
    Available Since
    April 9, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. PKM2 (3GQY)

    Plasmid
    #25360
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PKM2_1 (PKM Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 19, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. PANK1

    Plasmid
    #32871
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PANK1 (PANK1 Human)
    Use
    Tags
    His tag (with Thrombin cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 231-597 only
    Promoter
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. UBE2H

    Plasmid
    #25618
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    UBE2H (UBE2H Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acid residues 1-179
    Promoter
    Available Since
    Feb. 2, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. CBX6

    Plasmid
    #25296
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX6_02 (CBX6 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. GAMT

    Plasmid
    #25494
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GAMT (GAMT Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 821 - 840 of 1322 results