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pcDNA3.1_hE2F7mg_6ntmut

Plasmid

#73075

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

Depositor

Stefan Stamm

Publication

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns wi…

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_hE2F7mg_10ntmut

Plasmid

#73076

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

Depositor

Stefan Stamm

Publication

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns wi…

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-KAT14-1-546

Plasmid

#118377

Purpose

Gateway compatible donor vector with KAT14-1-546 truncation.

Depositor

Maria Vartiainen

Publication

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

KAT14 (KAT14 Human)

Use

Donor-plasmid

Mutation

Truncation (aa 1-546)

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pGS_N_Strep

Plasmid

#101651

Purpose

Expression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Publication

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, KpnI linker, Twin-Strep tag

Tags

Twin-Strep tag

Expression

Insect

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGS_C_Strep

Plasmid

#101652

Purpose

Expression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Publication

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, MCS linker, Twin-Strep tag

Tags

Twin-Strep tag

Expression

Insect

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSUPER-retro-puro-shNup88-XbaI

Plasmid

#87328

Purpose

To express shRNA against human Nup88

Depositor

Lei Lu

Publication

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup88

Use

RNAi

Expression

Mammalian

Promoter

H1

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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csd1_54-312/pET21a(+)

Plasmid

#83291

Purpose

csd1, construct: 54-312, pET21a(+) C-tag

Depositor

SeWon Suh

Publication

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1543

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-53

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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csd2_121-308/pET28b(+)

Plasmid

#83292

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag

Depositor

SeWon Suh

Publication

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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csd1_125-312/pET28b(+)

Plasmid

#83290

Purpose

csd1, construct: 125-312, pET28b(+) NC-tag

Depositor

SeWon Suh

Publication

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1543

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-124

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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Rat NCKX2-AS-long-FlagTag (pcDNA3.1+)

Plasmid

#75197

Purpose

Mammalian expression of SLC24A2

Depositor

Jonathan Lytton

Publication

Tsoi et al J Biol Chem. 1998 Feb 13;273(7):4155-62.

Insert

SLC24A2

Tags

FLAG

Expression

Mammalian

Mutation

alternatively spliced exon present

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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csd6_13-330_E110A/pET28b(+) N-tag

Plasmid

#71354

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:E110A

Depositor

SeWon Suh

Publication

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Glutamic acid 110 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP C1 SADBs shRNA Resistant

Plasmid

#67155

Purpose

pEGFP containing N-terminally EGFP Tagged mouse SADBs resistant to an shRNA

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Publication

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

Brsk1 (Brsk1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

nucleotide 294 C-T, nucleotide 297 C-G

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pIRES-Neo-HA-cdc25C5

Plasmid

#40967

Depositor

Olivier Gavet

Insert

cdc25C5 (CDC25C Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only
pET28A-p53 (1-73) (PPP)

Plasmid

#62067

Purpose

Expression of human p53 (1-73) (P12A, P13A, P27A) in e. coli

Depositor

Gary Daughdrill

Publication

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; P12A, P13A, P27A

Promoter

T7

Available since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
mTFP1-FYN-N-10

Plasmid

#55483

Purpose

Localization: Membrane Associated Kinase, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Publication

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

FYN (FYN Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-SiT-N-15

Plasmid

#55508

Purpose

Localization: Golgi TGN, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Publication

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

SiT (ST6GAL1 Human)

Tags

mTFP1

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
BDH2

Plasmid

#39151

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

BDH2 (BDH2 Human)

Tags

His Tev

Expression

Bacterial

Mutation

codon-optimized

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pLG-Acc

Plasmid

#33148

Depositor

Michael Rosbash

Publication

Legrain et al Cell. 1989 May 19;57(4):573-83.

Insert

RP51A-synthetic minimal intron (RPS17A Budding Yeast)

Tags

LacZ

Expression

Yeast

Mutation

AccI site in intron was cut/filled/ligated to cha…

Available since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pNTPTP alpha (Y789F)

Plasmid

#17702

Depositor

Xinmin Zheng

Publication

Zheng et al EMBO J. 2000 Mar 1. 19(5):964-78.

Insert

PTPalpha Y789F (PTPRA Human)

Expression

Mammalian

Mutation

Y789F

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pET-21a(+)-DR RecR(K23A/R27A)

Plasmid

#12619

Depositor

SeWon Suh

Publication

Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

Insert

Deinococcus radiodurans RecR (recR Deinococcus radiodurans)

Tags

His

Expression

Bacterial

Mutation

changed Lysine 23 to Alanine, and Arginine 27 to …

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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