We narrowed to 13,211 results for: CAR

1. HR-TERT-SV40-GFP

   Plasmid

   #71397

   Purpose

   Homologues recombination donor plasmid fo the TERT promotor region introducing a SV-40 driven GFP marker in the TERT promotor. Includes 1kb of TERT coding region in right homologues arm.

   Depositor

   Thomas Cech

   Article

   Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

   Insert

   TERT (TERT Human)
   

   Tags

   SV-40 GFP insertion in TERT promotor

   Promoter

   Endogenous TERT promotor

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ7748 mU6: Spy sgTET || CMV: mCherry~P2A-DD-A4

   Plasmid

   #123657

   Purpose

   DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control with sgRNA driving TRE3G activation.

   Depositor

   Stanley Qi

   Article

   Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

   Insert

   mCherry-P2A-DD-AcrIIA4
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   mCherry-P2A

   Expression

   Mammalian

   Promoter

   CMV and mU6

   Available Since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL4.23 WT 2014 EBOV Delta-Mucin-Like-Domain

   Plasmid

   #86021

   Purpose

   Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

   Depositor

   Jeremy Luban, Pardis Sabeti

   Article

   Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

   Insert

   Makona Ebola Virus Glycoprotein
   

   Expression

   Mammalian

   Mutation

   Lacks mucin-like domain

   Promoter

   CMV IE

   Available Since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSCGB3A2_HL-P2A-TagBFP-PGK-NeoR-SCGB3A2_HR

   Plasmid

   #126697

   Purpose

   donor vector for targeting a 2A peptide followed by blue fluorescent reporter to the human SCGB3A2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

   Insert

   TagBFP
   

   Expression

   Mammalian

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. SOX17-NLS-tdTomato-EPG

   Plasmid

   #210466

   Purpose

   Donor plasmid for knock-in NLS-tdTomato into the human SOX17 locus

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived puromycin-EGFP selection cassette (SOX17 Human)
   

   Use

   CRISPR

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-GFAP-eGFP-shLacZ

   Plasmid

   #223222

   Purpose

   mir30 based shRNA strategy, control shRNA

   Depositor

   Li-Huei Tsai

   Article

   Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

   Insert

   shLacZ
   

   Use

   AAV

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXs-WT-ATPIF1

   Plasmid

   #85403

   Purpose

   Retroviral vector expressing wild type human ATPIF1

   Depositor

   David Sabatini

   Article

   Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1

   Insert

   ATPIF1 (ATP5IF1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Wildtype

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pDGB3 alpha2 P35S:MS2:VPR:Tnos (GB1830)

   Plasmid

   #160624

   Purpose

   TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain VPR (VP64+p65+Rta)

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   P35S:MS2:VPR:Tnos
   

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

   Plasmid

   #126698

   Purpose

   to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

   Insert

   sgRNA targeting human SCGB3A2 locus
   

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEMS1991

    Plasmid

    #49122

    Purpose

    AAV plasmid with FEV (Ple67) promoter driving expression of iCre. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    ssAAV-Ple67-iCre-WPRE
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    FEV

    Available Since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. U6_sgRNA_CAG_hSt1Cas9_LMD9

    Plasmid

    #110626

    Purpose

    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA.

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    sgRNA for St1Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and hU6

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDGB3 alpha2 P35S:MS2:TV:Tnos (GB2048)

    Plasmid

    #160627

    Purpose

    TU for the constitutive expression of Ms2 fused to TV (TALx6 - VP128) activation domains

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35s-MS2:TV-Tnos
    

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-sgLkb1/Cre

    Plasmid

    #66894

    Purpose

    Expresses an Lkb1-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

    Insert

    sgLkb1
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. LV-hHOXA13

    Plasmid

    #135561

    Purpose

    Express hHOXA13 and EGFP in mammalian cells

    Depositor

    Jose Teixeira

    Article

    George et al Cell Rep. 2019 Dec 17;29(12):4069-4085.e

    Insert

    Homeobox A13 (HOXA13 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CBH

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pY036_ATP1A1_G5_Array

    Plasmid

    #86620

    Purpose

    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G5 crRNA + user-specified crRNA + AsCpf1-3xHA
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGBT9-Hook3

    Plasmid

    #198525

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-Hook3 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    Hook3 (HOOK3 Human)
    

    Tags

    GAL4-DNA binding domain (BD) fragment

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. APEX2-6xHp

    Plasmid

    #214411

    Purpose

    Lipid droplet localization of APEX2-6xHp for electron microscopy

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    6x Spastin aa 43-92 (APEX2 Human)
    

    Tags

    APEX2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7-G-LbCas12a crRNA-BsaI cassette (MSP3495)

    Plasmid

    #114068

    Purpose

    T7 promoter crRNA entry vector used for in vitro transcription of LbCas12a guides

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for LbCas12a crRNAs
    

    Use

    In vitro transcription

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTBL680 CHYRON3 integration construct

    Plasmid

    #126448

    Purpose

    To integrate the CHYRON3 locus at AAVS1 in human cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6/3xLacO-CHYRON3 hgRNA
    pCMV-puro
    

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6/3xLacO

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. eGFP Depdc5 pLJM1

    Plasmid

    #46380

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Differs from Depdc5 isoform 1 by 10 amino acids …

    Available Since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only