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We narrowed to 16,183 results for: nes

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Showing: 8421 - 8440 of 16183 results

  2. pCas9GG

    Plasmid
    #131009
    Purpose
    Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.
    Depositor
    Chase Beisel
    Publication
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of SpCas9
    promoter PJ23119
    mRFP expression cassette
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Bba_R0040 TetR
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131009-plasmid-map-sequence-id-257082

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  3. pMaCTag-Z14

    Plasmid
    #120057
    Purpose
    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeptune2.5
    Depositor
    Michael Knop
    Publication
    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mNeptune2.5
    Use
    Pcr template
    Tags
    mNeptune2.5
    Promoter
    None
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120057-plasmid-map-sequence-id-232666

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  4. pMaCTag-P04

    Plasmid
    #120015
    Purpose
    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mTFP1
    Depositor
    Michael Knop
    Publication
    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mTFP1
    Use
    Pcr template
    Tags
    mTFP1
    Promoter
    None
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120015-plasmid-map-sequence-id-232612

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  6. pJT9R

    Plasmid
    #98305
    Purpose
    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoter
    Depositor
    Claudia Vickers
    Publication
    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.
    Insert
    TRPL3AcNES1>TRPL41B
    Expression
    Yeast
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98305-plasmid-map-sequence-id-190176

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  9. lentiCRISPR - Amplicon, JAK2 sgRNA 2

    Plasmid
    #70660
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Publication
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. IQGAP2

    Plasmid
    #25172
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    IQGAP2:A1476-K1571 (IQGAP2 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids A1476-K1571
    Available since
    Aug. 9, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. pEasyG2_mic

    Plasmid
    #184916
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.
    Depositor
    Jeferson Gross
    Publication
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184916-plasmid-map-sequence-id-371408

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Showing: 8421 - 8440 of 16183 results