Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 14,187 results for: shi

Showing: 8441 - 8460 of 14187 results

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pBS-KS-attB2-SA(0)-T2A-3X Gal80-Hsp70

Plasmid

#62951

Purpose

Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 0. Gal80 is tagged with FLAG, HA and EGFP.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

3XGal80-FLAG/HA/EGFP-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-eYFP

Plasmid

#117383

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has Service

AAV1

Insert

EYFP

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-QF2-Hsp70

Plasmid

#62945

Purpose

Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-QF2-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 ALK4 T206D

Plasmid

#80880

Purpose

mammalian expression of ALK4 T206D

Depositor

Aristidis Moustakas, Peter ten Dijke

Article

ten Dijke et al Science. 1994 Apr 1;264(5155):101-4.

Insert

ALK4 (ACVR1B Human)

Tags

HA

Expression

Mammalian

Mutation

T206D

Promoter

CMV

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR223-BPLF1_2001-3000

Plasmid

#198586

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BPLF1_2001-3000

Use

Gateway entry vector

Mutation

None (wildtype)

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-BPLF1_1-1000

Plasmid

#198582

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BPLF1_1-1000

Use

Gateway entry vector

Mutation

None (wildtype)

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-BPLF1_501-1500

Plasmid

#198583

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BPLF1_501-1500

Use

Gateway entry vector

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BPLF1_1001-2000

Plasmid

#198584

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BPLF1_1001-2000

Use

Gateway entry vector

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BPLF1_1501-2500

Plasmid

#198585

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BPLF1_1501-2500

Use

Gateway entry vector

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BPLF1_3009-3149

Plasmid

#198587

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BPLF1_3009-3149

Use

Gateway entry vector

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-B-GD-RF1

Plasmid

#195829

Purpose

Gateway Entry Clone of Epstein-Barr virus BGRF1-BDRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BGRF1-BDRF1

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BFLF1

Plasmid

#195819

Purpose

Gateway Entry Clone of Epstein-Barr virus BFLF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BFLF1

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BVRF1

Plasmid

#195821

Purpose

Gateway Entry Clone of Epstein-Barr virus BVRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BVRF1

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BVRF2

Plasmid

#195823

Purpose

Gateway Entry Clone of Epstein-Barr virus BVRF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BVRF2

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BBRF1

Plasmid

#195826

Purpose

Gateway Entry Clone of Epstein-Barr virus BBRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BBRF1

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pRT498-3xOtUBD

Plasmid

#190088

Purpose

Expresses 3 tandem OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

Depositor

Mark Hochstrasser

Article

Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

Insert

3xOtUBD

Tags

6xHis and MBP

Expression

Bacterial

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAV126

Plasmid

#63192

Purpose

pRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMTKM30

Plasmid

#233502

Purpose

Level 0 plasmid for MoClo, encodes inducible TDH3-tetO2p for K. marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

TDH3-tetO2p

Use

Synthetic Biology

Expression

Yeast

Mutation

addition of two tetracycline operator sequeneces

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM31

Plasmid

#233503

Purpose

Level 0 plasmid for MoClo, encodes inducible TEF3-tetO2p for K. marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

PGK1-tetO2p

Use

Synthetic Biology

Expression

Yeast

Mutation

addition of two tetracycline operator sequeneces

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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FAB(ER-LD)

Plasmid

#214424

Purpose

Detection of ER-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

FRB-V5

Tags

CFAST-Halo and RspAN-FRB-V5

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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