We narrowed to 9,182 results for: Pol

1. pSN526

   Plasmid

   #184827

   Purpose

   Expresses human KIF5A(∆exon27) in insect cells

   Depositor

   Kyoko Chiba

   Article

   Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

   Insert

   KIF5A (∆exon27) (KIF5A Human)
   

   Tags

   mScarlet-2xStrepII

   Expression

   Insect

   Mutation

   ∆exon27 form of human KIF5A

   Promoter

   polyhedrin

   Available Since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. OTUB1/pRetroX-IRES-DsRed#10

   Plasmid

   #180279

   Purpose

   Plasmid encoding the human protease OTUB1 without a tag. Polycistronic red fluorescent retroviral vector (unmodified from TAKARA BIO).

   Depositor

   Andre Catic

   Article

   Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

   Insert

   human Otubain-1 (OTUB1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898

   Plasmid

   #105635

   Purpose

   Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1873-1898) from bicistronic plasmid

   Depositor

   Thanos Halazonetis

   Inserts

   Rev7 (Mad2l2 Mouse)
   Rev3l (REV3L Human)
   

   Expression

   Bacterial

   Mutation

   Mutations: F11S, G12A, V132K, C133V, A135K

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014

   Plasmid

   #105637

   Purpose

   Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1989-2014) from bicistronic plasmid

   Depositor

   Thanos Halazonetis

   Inserts

   Rev7 (Mad2l2 Mouse)
   Rev3l (REV3L Human)
   

   Expression

   Bacterial

   Mutation

   Mutations: F11S, G12A, V132K, C133V, A135K

   Promoter

   T7

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-mSpata33-PA

   Plasmid

   #179129

   Purpose

   Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

   Insert

   spermatogenesis associated 33 (Spata33 Mouse)
   

   Tags

   PA

   Expression

   Mammalian

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. IBMc397

   Plasmid

   #161955

   Purpose

   Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
   

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHis

   Plasmid

   #175788

   Purpose

   Plasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

   Insert

   Dispersin B
   

   Tags

   AviTag, Hexahistidine tag, and eGFP

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACEBac1-Q22YU3-2xStrep

   Plasmid

   #170315

   Purpose

   Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3 C-terminally tagged with 2x Strep-tag II

   Depositor

   Andrew Carter

   Article

   Mali et al Science. 2021 Feb 26;371(6532):910-916.

   Insert

   Q22YU3 (TTHERM_00122270 Tetrahymena thermophila)
   

   Tags

   Strep-Tag II

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP–acVHH–PB

   Plasmid

   #169685

   Purpose

   An anti-caffeine nanobody responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.

   Depositor

   Yubin Zhou

   Article

   Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214

   Insert

   anti-caffeine nanobody
   

   Tags

   modified STIM1-PB tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACEBac1-Q22YU3 del C3-2xStrep

    Plasmid

    #170316

    Purpose

    Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3 with a C-terminal truncation (C3-finger). Tagged with 2x Strep-tag II at the C-terminus.

    Depositor

    Andrew Carter

    Article

    Mali et al Science. 2021 Feb 26;371(6532):910-916.

    Insert

    Q22YU3 C-terminal truncation (Delta-C3)
    

    Tags

    Strep-Tag II

    Expression

    Insect

    Mutation

    Deletion of C-terminal C3-finger

    Promoter

    polyhedrin

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJet-CMV-CD5cmyc2-bglpA

    Plasmid

    #110645

    Purpose

    Expression plasmid for c-Myc-tag embedded into human CD52 with short polyA

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    CMV-CDcmyc2-bglpA
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOCC17

    Plasmid

    #118875

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal tagRFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-tagRFP-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and tagRFP

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOCC16

    Plasmid

    #118870

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal EGFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-EGFP-stop-HindIII cassette
    

    Tags

    EGFP and HIS6, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOCC218

    Plasmid

    #118907

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS10, cleavable with 3C protease and N-terminal YBBR tag

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS10-3C-YBBR-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS10, cleavable with 3C protease, YBBR tag

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOCC18

    Plasmid

    #118868

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal tagRFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-tagRFP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease, tagRFP

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOCC171

    Plasmid

    #118888

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP tag

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-SNAP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease; SNAP tag

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOCC202

    Plasmid

    #118880

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mKate2, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
    

    Tags

    mKate2, HIS6, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOCC139

    Plasmid

    #118872

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-mGFP-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and mGFP

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pK RagA(R37P)-RagC

    Plasmid

    #99706

    Purpose

    Bacterial expression of RagA(R37P)-RagC

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag, Poly Arg, and SUMO

    Expression

    Bacterial

    Mutation

    R37P

    Promoter

    T7

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pK RagA-RagC(L91P)

    Plasmid

    #99707

    Purpose

    Bacterial expression of RagA-RagC(L91P)

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag, Poly Arg, and SUMO

    Expression

    Bacterial

    Mutation

    L91P

    Promoter

    T7

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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