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pDIRECT_21J

Plasmid

#91132

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: 2x35S:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:TALEN

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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PMV-ycrtE

Plasmid

#65341

Purpose

systhesis of crtE gene in the beta-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

ycrtE

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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HOXA9 (murine) HIS-tag pET

Plasmid

#8492

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HoxA9 (Hoxa9 Mouse)

Use

Tags

His and T7

Expression

Bacterial

Mutation

BamHI/BalI fusion into amino acid #2 of HOXA9; ma…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pSUPER-retro-puro-shNup35-EcoRI

Plasmid

#87332

Purpose

To express shRNA against human Nup35

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup35

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCEP4-001428gp160

Plasmid

#123255

Purpose

Mammalian expression plasmid for Env from the 001428 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (001428) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pSuper.Retro.Puro-PKN1 shRNA 4

Plasmid

#59978

Purpose

Knockdown of PKN1

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

PKN1 shRNA

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only
pMOD_A0301

Plasmid

#91005

Purpose

Module A, Promoter: 35S, Gene: AtCas9_H840A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_H840A nickase

Use

CRISPR

Tags

Expression

Mutation

H840A

Promoter

35S

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSB1A2QS

Plasmid

#138268

Purpose

MINPA Gene circuit expression in E. coli. GFP serves as a reporter for Plux/tet promoter activity. mCherry serves as reporter for Para/lac promoter activity

Depositor

Xiao Wang

Article

Wu et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Inserts

AraC-TetR operon
LuxR-LacI operon
mCherry
GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Para/lac and Plux/tet

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCA0.001

Plasmid

#119557

Purpose

Level 0 acceptor vector for promoters truncated to transcriptional start site with lacZ compatible with blue/white screening

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/intron/FlagCter

Plasmid

#113548

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

Flag

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-STOP-shA1

Plasmid

#14422

Depositor

Klaus Rajewsky

Article

Oberdoerffer et al Mol Cell Biol. 2005 May . 25(10):3896-90

Insert

modified U6 promoter (U6lox), U6 STOP cassette, shA1 gene

Use

Cre/Lox and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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P315-FBP_10

Plasmid

#139701

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing CPH and Npga

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pUBQ1:NnH3H:tNos, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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XE28 XBC 40

Plasmid

#16842

Depositor

Randall Moon

Article

Yost et al Genes Dev. 1996 Jun 15. 10(12):1443-54.

Insert

beta-catenin-myc (ctnnb1.L Frog)

Use

Xenopus expression

Tags

6x myc

Expression

Mutation

Promoter

Available since

May 22, 2009

Availability

Academic Institutions and Nonprofits only
acta 2

Plasmid

#78711

Purpose

zebrafish acta2 3-UTR–specific probe

Depositor

Sarah Childs

Article

Georgijevic et al Dev Dyn. 2007 Jun;236(6):1623-32.

Insert

acta2 (acta2 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

contains bp 1,243–1,591

Promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
pMC8b-XI3up

Plasmid

#176170

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-3

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site XI-3

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC7-XI3dw

Plasmid

#176171

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-3

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site XI-3

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC8b-XI5up

Plasmid

#176172

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-5

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site XI-5

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC7-XI5dw

Plasmid

#176173

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-5

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site XI-5

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC8b-XII5up

Plasmid

#176176

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XII-5

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site XII-5

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC7-XII5dw

Plasmid

#176177

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-5

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site XII-5

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge