We narrowed to 18,493 results for: Met

1. RNA-Protein Landing Pad

   Plasmid

   #226162

   Purpose

   Used to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expression

   Depositor

   Dustin Maly

   Article

   Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

   Inserts

   mTagBFP2-P2A-iCasp9
   Reverse tetracycline transactivator 3
   Hygromycin-IRES-EGFP
   

   Use

   Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEGFP-C1-Nup35

   Plasmid

   #87342

   Purpose

   To express human Nup35 in mammalian cells

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   nucleoporin 35 (NUP35 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPAmCherry2-C1

   Plasmid

   #31932

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

   Insert

   PAmCherry2
   

   Expression

   Mammalian

   Mutation

   M18L/K69N/L84F/A147T/S148L/I165L/Q167A/L169T/A179…

   Promoter

   CMV

   Available Since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDule-Tet3.0

   Plasmid

   #174080

   Purpose

   Tetrazine 3.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

   Depositor

   Ryan Mehl

   Article

   Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

   Insert

   Tet3.0 tRNA synthetase and tRNA
   

   Expression

   Bacterial

   Mutation

   L270G, Y271L, N311G, C313A

   Available Since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPT-TAR C1

   Plasmid

   #141246

   Purpose

   Transformation Associated Recombination (TAR) cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Algal Research, 2020. 50, 102014

   Insert

   Mitochondrial Genome of Phaeodactylum tricornutum
   

   Use

   Synthetic Biology; Diatom expression

   Expression

   Bacterial and Yeast

   Mutation

   Minor additional mutations described in manuscript

   Available Since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJM456 EF1alpha EBFP2-P2A-BlastR (TUPV7)

   Plasmid

   #139236

   Purpose

   mMoClo TUPV7, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   EBFP2-P2A-Blasticidin S Deaminase
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-28a(+)::NL

   Plasmid

   #141289

   Purpose

   NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   NLUC
   

   Use

   Luciferase

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBF3060

   Plasmid

   #26853

   Purpose

   Expression of yeast codon-optimized CreA recombinase from the GAL1 promoter

   Depositor

   Nancy DaSilva, Suzanne Sandmeyer

   Article

   Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

   Insert

   CreA recombinase
   

   Use

   Cre/Lox

   Expression

   Yeast

   Mutation

   N/A

   Promoter

   GAL1

   Available Since

   Dec. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRK5-HA-SAMTOR

   Plasmid

   #100513

   Purpose

   Expresses HA tagged SAMTOR in mammalian cells

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   SAMTOR (BMT2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pClathrin-miRFP670nano3

    Plasmid

    #184667

    Purpose

    Clathrin labelled with near-infrared fluorescent protein miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    miRFP670nano3-clathrin light chain
    

    Tags

    miRFP670nano3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Salsa-BC96-P1H12

    Plasmid

    #202937

    Purpose

    Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC96

    Depositor

    Noah Palm

    Article

    Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

    Insert

    EGFP/BC96
    

    Expression

    Mammalian

    Promoter

    Tet Response Element (TRE)

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TLCV2-sgHPDL #3

    Plasmid

    #174165

    Purpose

    knock out HPDL in human cell lines

    Depositor

    Michael Pacold

    Article

    Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

    Insert

    sgRNA against HPDL
    

    Use

    Lentiviral

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TLCV2-sgHPDL #9

    Plasmid

    #174166

    Purpose

    knock out HPDL in human cell lines

    Depositor

    Michael Pacold

    Article

    Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

    Insert

    sgRNA against HPDL
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mEos2-ER-5

    Plasmid

    #57373

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    ER (CALR Human)
    

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    ER Signal Peptide

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. mtsCOXVIIIx2-B-gTEMP/pcDNA3

    Plasmid

    #199806

    Purpose

    Fluorescent temperature indicator B-gTEMP fused with mitochondrial targeting sequence

    Depositor

    Takeharu Nagai

    Article

    Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

    Insert

    B-gTEMP
    

    Tags

    mtsCOXVIIIx2

    Expression

    Mammalian

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-C1-FLAG-XRCC4

    Plasmid

    #46959

    Purpose

    Plasmid for expression of GFP-FLAG tagged human XRCC4 isoform 2 (N-terminal tag). Confers resistance to G418.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    XRCC4 (XRCC4 Human)
    

    Tags

    GFP-2xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAD-IRP

    Plasmid

    #220751

    Purpose

    Plasmid expresses the rabbit Iron Regulatory Protein fused to the Gal 4 Activation Domain.

    Depositor

    Marvin Wickens

    Article

    Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

    Insert

    Iron Regulatory Protein (ACO1 )
    

    Expression

    Yeast

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti6.3-enCjCas9-BlastR

    Plasmid

    #220978

    Purpose

    Expresses human codon-optimized enCjCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    enCjCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCX-mutSpiCee

    Plasmid

    #140903

    Purpose

    mammalian expression of a mutated form of SpiCee, that does not affect calcium signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    mutSpiCee
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. #1.4 post-G-SynapShot-mScarlet

    Plasmid

    #214716

    Purpose

    Expresses green #1.4 post-SynapShot and mScarlet

    Depositor

    Won Do Heo

    Article

    Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

    Insert

    #1.4 post-G-SynapShot
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge