165,528 results

1. TOMM20-PuRRRE

   Plasmid

   #208329

   Purpose

   PuRRRE genetically-encoded tag introduced after TOMM20; to be used with PunyEEER probe peptide.

   Depositor

   Kimberly Beatty

   Article

   Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

   Insert

   TOMM20-PuRRRE
   

   Tags

   PuRRRE

   Expression

   Mammalian

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBVboostFGII WPRE 2A-CVB3

   Plasmid

   #202076

   Purpose

   Bacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

   Depositor

   Vesa Hytönen

   Article

   Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

   Insert

   CVB3 2A protease with HisTag
   

   Tags

   6xHis-tag

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   polh, CAG, T7

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJR017

   Plasmid

   #198835

   Purpose

   In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::TeTX-SL2-mKate::let-858 3'UTR
   

   Expression

   Worm

   Available Since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-Spe4-Kozak-miRFP713-RfA

   Plasmid

   #186381

   Purpose

   Destination vector for the expression of a fluorescent fusion of N-ter miRFP713 and a protein of interest into embryos, by mRNA microinjection

   Depositor

   Patrick Lemaire

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDx_mCherry-SYFP2

   Plasmid

   #189759

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mCherry-SYFP2 fusion protein for FRET analysis

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mCherry-SYFP2
   

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Promoter

   CMV & Rha

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. SFRS10

   Plasmid

   #156007

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   SFRS10 (TRA2B Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. F40F8.11 RNAi

   Plasmid

   #186731

   Purpose

   Knockdown of F40F8.11 gene

   Depositor

   Seung-Jae Lee

   Insert

   F40F8.11 targeting RNA
   

   Use

   RNAi

   Available Since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST-Spe4-RfA-miRFP713-stop

   Plasmid

   #186391

   Purpose

   Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter miRFP713 into embryos, by mRNA microinjection

   Depositor

   Patrick Lemaire

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-SKII-3XHA-eDHFR-NAT

   Plasmid

   #188926

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-eDHFR-NAT
   

   Expression

   Yeast

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB-Halo,IRES-NGFR

    Plasmid

    #164520

    Purpose

    All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

    Depositor

    Mitchell Guttman

    Article

    Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV_2pabPAC(Y72L)

    Plasmid

    #158970

    Purpose

    A negative control for AAV-mediated 2P-activatable bPAC

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

    Insert

    2pabPACY72L
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLEX-uORF-HA-birA*-N-Ras(Q61K/T35A)-IRES-Puro

    Plasmid

    #120565

    Purpose

    Expresses HA-birA*-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K, T35A

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLEX-uORF-HA-birA*-N-Ras(Q61K/Y40A)-IRES-Puro

    Plasmid

    #120567

    Purpose

    Expresses HA-birA*-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K, Y40A

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX-uORF-HA-birA*-N-Ras(Q61K/E37A)-IRES-Puro

    Plasmid

    #120566

    Purpose

    Expresses HA-birA*-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K, E37A

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Xl-VSP1 R152Q

    Plasmid

    #51881

    Purpose

    expression of Xl-VSP1 mutant in Xenopus oocytes or mammalian cells

    Depositor

    Laurinda Jaffe

    Article

    Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

    Insert

    VSP1 R152Q (tpte2 Frog)
    

    Use

    Xenopus oocyte expression

    Expression

    Mammalian

    Mutation

    R152Q: near the extracellular side of the fourth …

    Promoter

    CMV

    Available Since

    May 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. FU-tetO-Gateway

    Plasmid

    #43914

    Depositor

    John Gearhart

    Article

    Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    6xHis and V5

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBSJL1

    Plasmid

    #71261

    Purpose

    E. coli-Veillonella shuttle plasmid

    Depositor

    Felicia Qi

    Article

    Liu et al Appl Environ Microbiol. 2012 May;78(9):3

    Type

    Empty backbone

    Use

    E. coli-veillonella shuttle plasmid

    Expression

    Bacterial

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

18. mEmerald-Fascin-N-10

    Plasmid

    #54095

    Purpose

    Localization: Actin Bundling Protein, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Fascin (FSCN1 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pEGFP C3-YAP2

    Plasmid

    #19055

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.3_OCT4

    Plasmid

    #26816

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

    Insert

    5'UTR-OCT4-3'UTR (POU5F1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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