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LDB59

Plasmid

#185436

Purpose

NUP1 CEN URA3 plasmid

Depositor

Ken Belanger, Laura Davis

Article

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Expression

Yeast

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pUC57-Peft-pHCountdown-unc54

Plasmid

#182304

Purpose

C. elegans plasmid encoding pH-Countdown with three introns

Depositor

Andrew York

Article

Seidel et al (2021), doi: 10.5281/zenodo.5810930

Insert

pH countdown with 3 introns, with a Peft3 promoter and unc54 terminator

Expression

Worm

Promoter

Peft-3

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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mEYFP-(L)-mApple

Plasmid

#182860

Purpose

Hetero-dimer expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

mEYFP-mApple

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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mEGFP-(L)-mApple

Plasmid

#182856

Purpose

Hetero-dimer expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

mEGFP-mApple

Expression

Mammalian

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-P3-cTVMVP

Plasmid

#179663

Purpose

Uncaged-cTVMVP with P3 leucine zipper

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

cTVMVP

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pVB002

Plasmid

#127186

Purpose

Tunable NOT gate with RiboJ insulator

Depositor

Thomas Gorochowski

Article

Bartoli et al Nat Commun. 2020 Apr 29;11(1):2095. doi:

Insert

Tunable NOT gate with RiboJ and Reporter

Expression

Bacterial

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a SznF E281A

Plasmid

#139591

Purpose

expresses SznF E281A

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF E281A

Tags

hexahistidine

Expression

Bacterial

Mutation

changed glutamate 281 to alanine

Promoter

T7

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a SznF H225A

Plasmid

#139590

Purpose

expresses SznF H225A

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF H225A

Tags

hexahistidine

Expression

Bacterial

Mutation

changed histidine 225 to alanine

Promoter

T7

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-SznF E215A

Plasmid

#139565

Purpose

expresses SznF E215A in E. coli

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF E215A

Tags

hexahistidine

Expression

Bacterial

Mutation

changed glutamate 215 to alanine

Promoter

T7

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-SznF

Plasmid

#139564

Purpose

expresses SznF in E. coli

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF

Expression

Bacterial

Promoter

T7

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAW8_tdTomato

Plasmid

#110239

Purpose

Cre/Lox C-terminal tagging construct encoding red fluorescent protein tdTomato

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_GST

Plasmid

#110237

Purpose

Cre/Lox C-terminal tagging construct encoding glutathione S-transferase

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_6XHis

Plasmid

#110236

Purpose

Cre/Lox C-terminal tagging construct encoding six histidine (His) residues

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_mCherry

Plasmid

#110238

Purpose

Cre/Lox C-terminal tagging construct encoding red fluorescent protein mCherry

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pHH04C_Sp_CoD

Plasmid

#120899

Purpose

the component plasmid of a unregulated device

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

Insert

L3S3P11 terminator

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_13Myc

Plasmid

#110232

Purpose

Cre/Lox C-terminal tagging construct encoding 13 copies of the human c-myc (Myc) epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_yEGFP

Plasmid

#110229

Purpose

Cre/Lox C-terminal tagging construct encoding codon-optimized green fluorescent protein

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_1XFLAG

Plasmid

#110234

Purpose

Cre/Lox C-terminal tagging construct encoding single copy of the FLAG epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_Xho

Plasmid

#110223

Purpose

Cre-expression plasmid for cassette exchange but MCS modified compared to pAW8

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_TAP

Plasmid

#110240

Purpose

Cre/Lox C-terminal tagging construct encoding the Twin Affinity Purification protein

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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