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We narrowed to 171,442 results for: Gene

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Showing: 8541 - 8560 of 171442 results

  2. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196078
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196078-plasmid-map-sequence-id-388989

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  4. pGBT-RP2.2

    Plasmid
    #189842
    Purpose
    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 2.
    Depositor
    Stephen Ekker
    Article
    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    GFP GM2 variant and mRFP
    Promoter
    beta Actin
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    189842-plasmid-map-sequence-id-373952

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  5. pcDNA-dCas9-p300 Core (Y1467F)

    Plasmid
    #61362
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; Y1467F mutation i…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61362-plasmid-map-sequence-id-99796

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  9. HS_NAM-B1

    Plasmid
    #154064
    Purpose
    Level M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosT
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Inserts
    Hygromycin phosphotransferase
    Cre recombinase
    LoxP-flanked GUS and TtNAM-B1
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Expression
    Bacterial and Plant
    Mutation
    The TtNAM-B1 gene sequence was domesticated to re…
    Promoter
    HvHSP17, OsAct, and ZmUbi
    Available Since
    Sept. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154064-plasmid-map-sequence-id-313309

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  10. pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196076
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196076-plasmid-map-sequence-id-388979

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  11. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196074
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196074-plasmid-map-sequence-id-389001

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  13. pGBT-RP2.3

    Plasmid
    #189843
    Purpose
    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 3.
    Depositor
    Stephen Ekker
    Article
    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    GFP GM2 variant and mRFP
    Promoter
    beta Actin
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    189843-plasmid-map-sequence-id-373951

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Showing: 8541 - 8560 of 171442 results