We narrowed to 15,735 results for: LEA

1. pSBbi-Puro_LPAR_AKAR-WT

   Plasmid

   #125206

   Purpose

   SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporter

   Depositor

   Eric Davis

   Insert

   LPAR_AKAR-WT
   

   Use

   Transposon

   Tags

   EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. 5C8G

   Plasmid

   #69968

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   Tb427tmp.01.1830:MAC054-B05:C234722
   

   Tags

   His and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO

   Available Since

   Oct. 7, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

3. Clim-DC1-mCherry-H2B-Venus

   Plasmid

   #129559

   Purpose

   nuclear marker

   Depositor

   Inaki Ruiz-Trillo

   Insert

   mcherry and Histone 2B fused to venus
   

   Expression

   Bacterial

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAS1-rab-3P::his-24::mCherry

   Plasmid

   #124348

   Purpose

   Nuclear Localized Pan-Neuronal mCherry

   Depositor

   Andrew Leifer

   Insert

   rab-3, mCherry (rab-3 Nematode)
   

   Expression

   Worm

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET His6 GSSGSS NusA TEV LIC cloning vector (2NTL)

   Plasmid

   #55214

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, GSSGSS linker, NusA, and TEV cleavage site…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. PLXNA2

   Plasmid

   #28279

   Depositor

   Cheryl Arrowsmith

   Insert

   PLXNA2 (PLXNA2 Human)
   

   Tags

   His with TEV cleavage

   Expression

   Bacterial

   Mutation

   residues 1490-1600.

   Available Since

   Feb. 10, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pcDNA3.1-hRIPK1-Flag-K599R

   Plasmid

   #112490

   Purpose

   Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

   Depositor

   Junying Yuan

   Insert

   RIPK1 (RIPK1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K599R

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-hRIPK1-Flag-K115R

   Plasmid

   #112488

   Purpose

   Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

   Depositor

   Junying Yuan

   Insert

   RIPK1 (RIPK1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K115R

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Top7 K39E K40E V48V K55E

   Plasmid

   #12464

   Purpose

   Plasmid contains Top7 K39E K40E V46A and K55E. Please note this is DIFFERENT than what the plasmid name implies.

   Depositor

   David Baker

   Insert

   Top7 K39E K40E V46A K55E
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K39E K40E V46A K55E

   Available Since

   March 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-Flag-HeFSpCas9-H840A

    Plasmid

    #80456

    Purpose

    high fidelity enhanced SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Insert

    HeFSpCas9_H840A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. mP_ILB_Vector 1

    Plasmid

    #232193

    Purpose

    The plasmid vector designed for the expression of four genes in oleaginous yeasts, including Yarrowia lipolytica and Rhodotorula toruloides.

    Depositor

    Matrika Bhattarai

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    March 3, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. 1203T-CMV-Cas7.1-NLS-bGHpolyA

    Plasmid

    #221540

    Purpose

    Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector), nuclear localization signal sequence on 5' and 3' end of the coding domain

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFHMSPN-avi-TEV-LIC V

    Plasmid

    #220885

    Purpose

    Donor vector to generate recombinant baculovirus by site-specific transposition in an E. coli host.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Baculovirus expresssion

    Tags

    Honeybee melittin signal followed by His, avi and…

    Available Since

    June 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. p_L_3c_SG_RLuc

    Plasmid

    #208729

    Purpose

    For assessing leaky scanning rates in mammalian cells

    Depositor

    Andrew Firth

    Insert

    ORF3c (ORF3a )
    

    Tags

    Renilla luciferase

    Expression

    Mammalian

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pnEA-NvG-DmNot3_677-844_P

    Plasmid

    #147185

    Purpose

    Bacterial Expression of DmNot3_677-844

    Depositor

    Elisa Izaurralde

    Insert

    DmNot3_677-844 (Not3 Fly)
    

    Expression

    Bacterial

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pnEA-NvM-DmNot3_677-844_P

    Plasmid

    #147190

    Purpose

    Bacterial Expression of DmNot3_677-844

    Depositor

    Elisa Izaurralde

    Insert

    DmNot3_677-844 (Not3 Fly)
    

    Expression

    Bacterial

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETM-60-DmNot3_677-844_P

    Plasmid

    #147177

    Purpose

    Bacterial Expression of DmNot3_677-844

    Depositor

    Elisa Izaurralde

    Insert

    DmNot3_677-844 (Not3 Fly)
    

    Expression

    Bacterial

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pExp-TFSumo

    Plasmid

    #112576

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli linked to Sumo) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8xHis-TFSumo and Strep tag

    Expression

    Bacterial

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pExp-Tsf

    Plasmid

    #112578

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Tsf (elongation factor Ts from E. coli fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8xHis-Tsf and Strep tag

    Expression

    Bacterial

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pExp-AFV

    Plasmid

    #112560

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and AFV (Acidianus Filamentous Virus 1 protein) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8xHis-AFV and Strep tag

    Expression

    Bacterial

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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