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Plasmid#74599Purposeused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523DepositorInserthuman dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
UseTagsN-terminal GST and Strep IIExpressionBacterialMutationamino acids 389-523 of LIC1PromoterT7Available sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDB-hPER2-Halo-CD4-bla
Plasmid#179451PurposeDonor vector to knock in Halotag C-terminal to human PER2 geneDepositorInsertHaloTag (Halo7 Synthetic)
UseCRISPRTagsHis/FlagExpressionMutationPromoterNoneAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Dppa1-T2A-H2B-Halo donor
Plasmid#113118PurposeDonor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequenceDepositorInsertDppa1 donor region including homology arms and T2A-H2B-Halo
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-hH2A-(GGS)X6-HaloTag C58
Plasmid#171431PurposeExpression of hH2A-(GGS)X6-HaloTag C58 in mammalian cellsDepositorInserthH2A-(GGS)X6-HaloTag C58
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-SspB-HaloTag-TRIO-DHPH1
Plasmid#176126PurposeHeterodimerization with iLID, Rac1 and RhoG activationDepositorInsertTRIO (TRIO Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-KIF13A tail 361-1749
Plasmid#162720PurposeMammalian expression of WT mouse Kif13A with deletion of the 360 N-terminal residuesDepositorInsertKif 13A (Kif13a Mouse)
UseTagsExpressionMammalianMutationWT with deletion of the 360 N-terminal residues.PromoterChicken Beta actinAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-ALFANb-HaloTag-GB1-dWPRE
Plasmid#231600PurposeExpress ALFANb-HaloTagDepositorInsertALFANb-HaloTag
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
EP004: pAAV TRE::GEM7-HaloTag
Plasmid#209304PurposeDoxycycline-gated expression plasmid (TetOFF) for GEM7 (encapsulin from R. rubrum) with C-terminal HaloTag suitable for expression in miceDepositorInsertGEM7
UseAAVTagsHaloTagExpressionMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Homology Arms-BRD4-HaloTag
Plasmid#228575PurposeCRISPR donor plasmid to tag human BRD4 with Halo tag; for CRISPR-mediated gene insertionDepositorInsertHomology Arms-BRD4-HaloTag (BRD4 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330-U6-BRD4-HaloTag-Guide-hspCas9
Plasmid#228576PurposeExpression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertionDepositorInsertBRD4 gRNA (BRD4 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IBU-pSu9-HaloTag7-SNAP
Plasmid#217319PurposeExpresses the presequence of Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag for measuring mitophagy activity by Halo processing assay.DepositorInsertNeurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag
UseRetroviralTagsHaloTag and SNAPExpressionMutationPromoterCMVAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-Halo-PILATeS-RGD (200nM)
Plasmid#216716PurposeExpresses HaloTag-PILATeS-RGD with 200 nM BiT tag in bacteriaDepositorInsertHaloTag-PILATeS-RGD
UseTags6xHis and HaloTagExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-Halo-PILATeS-RGD (700pM)
Plasmid#216714PurposeExpresses HaloTag-PILATeS-RGD with 700 pM BiT tag in bacteriaDepositorInsertHaloTag-PILATeS-RGD
UseTags6xHis and HaloTagExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-MDC1 BRCT domain deletion
Plasmid#207110PurposepHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 with a deletion of the BRCT domain in human cells.DepositorInsertHaloTag-MDC1 BRCT domain deletion
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP
Plasmid#179717PurposeLentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFPDepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hPER1-Halo-CD4-bla
Plasmid#189982PurposeDonor Vector to integrate HaloTag C-terminal into the human PER1 Locus to express it as a fusion proteinDepositorInsertHaloTag
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hCRY2-Halo-CD4-bla
Plasmid#189986PurposeDonor Vector to integrate HaloTag C-terminal into the human CRY2 Locus to express it as a fusion proteinDepositorInsertHaloTag
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-XPACK-DNAJC5 (L115R)- HaloTag
Plasmid#205712PurposeMammalian expression of XPACK-DNAJC5 (L115R)-HaloTagDepositorInsertDNAJC5 (DNAJC5 Human)
UseTagsHaloTag and XPACK (GCINSKRKD)ExpressionMammalianMutationL115RPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-V5-HisB-ObKHC-K282R-HaloTag
Plasmid#201552PurposeExpresses Octopus kinesin-1 motor with K282R mutation with HaloTag in Drosophila S2 cellsDepositorInsertKinesin-1
UseTagsHaloTagExpressionInsectMutationchanged lysine 282 to arginine (numbering based o…PromoterAc5Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.HALO-KIF3A tail 419-702
Plasmid#193924PurposeMammalian expression of Halo-tagged Kif 3A tail 419-702DepositorInsertKif 3A (KIF3A Human)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only