We narrowed to 29,515 results for: REP

1. pFsynW SYT1-PGM reporter

   Plasmid

   #197278

   Purpose

   Encodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.

   Depositor

   Edwin Chapman

   Article

   Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

   Insert

   SYT1 and HaloTag (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   T15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82A

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOET3-C-TEV-Twin-Strep

   Plasmid

   #203324

   Purpose

   Generate the baculovirus to express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cells

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   TEV cleavable site (ENLYFQG) and Twin-strep-tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-REPORT(EF1a no GFP)

   Plasmid

   #172339

   Purpose

   mnucTomato

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-REPORT(EF1a)TetR

   Plasmid

   #172352

   Purpose

   Tet Repressor reporter

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   TetR
   d2eGFP
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pnEA-NpG-HsNanos1_40-56-Strep_W

   Plasmid

   #147824

   Purpose

   Bacterial Expression of HsNanos1_40-56

   Depositor

   Elisa Izaurralde

   Article

   Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

   Insert

   HsNanos1_40-56 (NANOS1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pnEA-NpG-HsNanos3_4-20-Strep_W

   Plasmid

   #147825

   Purpose

   Bacterial Expression of HsNanos3_4-20

   Depositor

   Elisa Izaurralde

   Article

   Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

   Insert

   HsNanos3_4-20 (NANOS3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pnEA-NpM-HsNanos3_4-20-Strep_W

   Plasmid

   #147826

   Purpose

   Bacterial Expression of HsNanos3_4-20

   Depositor

   Elisa Izaurralde

   Article

   Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

   Insert

   HsNanos3_4-20 (NANOS3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pnYC-NpG-HsNanos2_6-22-Strep_W

   Plasmid

   #147828

   Purpose

   Bacterial Expression of HsNanos2_6-55

   Depositor

   Elisa Izaurralde

   Article

   Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

   Insert

   HsNanos2_6-55 (NANOS2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pN23-LIC-FLAG-STREP

   Plasmid

   #182508

   Purpose

   pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and STREP

   Depositor

   Renato Bruni

   Type

   Empty backbone

   Use

   Tags

   STREP-FLAG-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pACE-Truncated-Kir3.2-StreptagII

    Plasmid

    #177263

    Purpose

    Expresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cells

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al J Phys Chem Lett. 2021 Dec 30;12(51):122

    Insert

    G-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)
    

    Use

    Tags

    StreptagII

    Expression

    Insect

    Mutation

    Residues 49-378

    Promoter

    polyhedrin promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-WIPI2dR108E-cs-TEV-STREP

    Plasmid

    #171835

    Purpose

    Mammalian expression of human WIPI2d R108E with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    R108E

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-WIPI2dR125E-cs-TEV-STREP

    Plasmid

    #171836

    Purpose

    Mammalian expression of human WIPI2d R125E with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    R125E

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-WIPI2dK128E-cs-TEV-STREP

    Plasmid

    #171837

    Purpose

    Mammalian expression of human WIPI2d K128E with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    K128E

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG-WIPI2dH85E-cs-TEV-STREP

    Plasmid

    #171831

    Purpose

    Mammalian expression of human WIPI2d H85E with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    H85E

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-WIPI2dK88E-cs-TEV-STREP

    Plasmid

    #171832

    Purpose

    Mammalian expression of human WIPI2d K88E with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    K88E

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-WIPI2dI92E-cs-TEV-STREP

    Plasmid

    #171833

    Purpose

    Mammalian expression of human WIPI2d I92E with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    I92E

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-WIPI2dC93E-cs-TEV-STREP

    Plasmid

    #171834

    Purpose

    Mammalian expression of human WIPI2d C93E with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    C93E

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 6xHis(TEV)-yDrp1-StrepII

    Plasmid

    #174427

    Purpose

    Expresses S. pombe Drp1 in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Use

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACEBac1-Q22YU3 del C3-2xStrep

    Plasmid

    #170316

    Purpose

    Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3 with a C-terminal truncation (C3-finger). Tagged with 2x Strep-tag II at the C-terminus.

    Depositor

    Andrew Carter

    Article

    Mali et al Science. 2021 Feb 26;371(6532):910-916.

    Insert

    Q22YU3 C-terminal truncation (Delta-C3)
    

    Use

    Tags

    Strep-Tag II

    Expression

    Insect

    Mutation

    Deletion of C-terminal C3-finger

    Promoter

    polyhedrin

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLJM1-zeo-2xStrep-ORF68

    Plasmid

    #162659

    Purpose

    Lentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Lentiviral

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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