Skip to main content
Addgene
Showing: 1681 - 1720 of 4899 results

  1. pwAB-erg8erg19idi1erg20

    Plasmid
    #227345
    Purpose
    Overexpression of the erg8, erg19, idi1, and erg20 genes in Aspergillus oryzae
    Depositor
    Jun-ichi Maruyama
    Insert
    Expression cassettes of the erg8, erg19, idi1, and erg20 genes
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    227345-plasmid-map-sequence-id-458568

    Enlarge

  2. pdadhDN-tefAO217

    Plasmid
    #227341
    Purpose
    Replacement of the adh gene with the expression cassette of the AO217 gene
    Depositor
    Jun-ichi Maruyama
    Insert
    Flanking regions of adh (AO090009000634) and expression cassette of AO217 (AO090120000217)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    227341-plasmid-map-sequence-id-458620

    Enlarge

  3. ppG-ple12

    Plasmid
    #227339
    Purpose
    Heterologous production of pleuromutilin in Aspergillus oryzae
    Depositor
    Jun-ichi Maruyama
    Insert
    The upstream/downstream regions of the pyrG gene and expression cassettes of the ple1 and ple2 genes
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    227339-plasmid-map-sequence-id-458622

    Enlarge

  4. pLys57-2

    Plasmid
    #211565
    Purpose
    Amber Suppressor tRNA for Lysine (Methionine-derived)
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNALys
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211565-plasmid-map-sequence-id-421056

    Enlarge

  5. pLys57-3

    Plasmid
    #211566
    Purpose
    Amber Suppressor tRNA for Lysine (Isoleucine-derived)
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNALys
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211566-plasmid-map-sequence-id-421057

    Enlarge

  6. pLys57-4

    Plasmid
    #211567
    Purpose
    Amber Suppressor tRNA for Lysine (Aspartate-derived)
    Depositor
    Aron Fenton, Liskin Swint-Kruse
    Insert
    tRNALys
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211567-plasmid-map-sequence-id-421059

    Enlarge

  7. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)Cy-

    Plasmid
    #191390
    Purpose
    Donor for targeted integration of hCdt1(1-100)Cy- probe to the hROSA26 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hCdt1(1-100)Cy-
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191390-plasmid-map-sequence-id-378098

    Enlarge

  8. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)

    Plasmid
    #191389
    Purpose
    Donor for targeted integration of hCdt1(1-100) probe to the hROSA26 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hCdt1(1-100)
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191389-plasmid-map-sequence-id-378099

    Enlarge

  9. CAG-Mermaid in Tol2

    Plasmid
    #182578
    Purpose
    Expresses the FRET membrane voltage sensor Mermaid in vertebrate cells
    Depositor
    Andreas Stuermer
    Insert
    CAG-Mermaid in Tol2
    Use
    Zebrafish expression
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182578-plasmid-map-sequence-id-358930

    Enlarge

  10. pTOX-donor

    Plasmid
    #132516
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    TOX (TOX Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    none
    Promoter
    Available since
    March 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    132516-plasmid-map-sequence-id-274812

    Enlarge

  11. pIAeBlue

    Plasmid
    #185891
    Purpose
    In vivo gene amplification (HamAmp) of AeBlue expression module at RPL25 locus
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>AeBlue>TPGK1- PBTS1> RPL25(partial; Arm2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185891-plasmid-map-sequence-id-370863

    Enlarge

  12. pAW8-aid-Turg1:kanMX6

    Plasmid
    #169354
    Purpose
    C-terminal aid degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169354-plasmid-map-sequence-id-333202

    Enlarge

  13. pAW8-aid-V5-Turg1:kanMX6

    Plasmid
    #169357
    Purpose
    C-terminal aid-V5 degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    1XV5
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169357-plasmid-map-sequence-id-333200

    Enlarge

  14. pTBX18-donor

    Plasmid
    #176370
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    TBX18 (TBX18 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176370-plasmid-map-sequence-id-347398

    Enlarge

  15. pIEforRed

    Plasmid
    #185892
    Purpose
    In vivo gene amplification (HamAmp) of EforRed expression module at RPL25 locus
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>EforRed>TPGK1-PBTS1> RPL25(partial; Arm2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185892-plasmid-map-sequence-id-370865

    Enlarge

  16. pJK678

    Plasmid
    #72376
    Purpose
    Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    phosphoribosyl pyrophosphate amidotransferase (PPAT Human)
    Use
    Gene synthesis
    Tags
    Expression
    Mutation
    Promoter
    n/a
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72376-plasmid-map-sequence-id-138130

    Enlarge

  17. pJG01

    Plasmid
    #213505
    Purpose
    expresses TdTomato in Capsaspora owczarzaki
    Depositor
    Joseph Gerdt
    Insert
    TdTomato
    Use
    Tags
    Expression
    Mutation
    Promoter
    EF1
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    213505-plasmid-map-sequence-id-431905

    Enlarge

  18. pZNF468-donor

    Plasmid
    #176350
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ZNF468 (ZNF468 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    176350-plasmid-map-sequence-id-347397

    Enlarge

  19. pJK588

    Plasmid
    #72270
    Purpose
    Synthetic gene corresponding to Synechocystis PCC6803 orf sll1489
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein SYNGTS_3005
    Use
    Gene cloning
    Tags
    His6 and TEV cleavage site
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72270-plasmid-map-sequence-id-131740

    Enlarge

  20. pLinker-2xTy-HXGPRT-LoxP

    Plasmid
    #86664
    Purpose
    CRISPR tagging with 2xTy in Toxoplamsa gondii
    Depositor
    David Sibley
    Insert
    Linker-2xTy-HXGPRT 3'UTR
    Use
    Expression in toxoplasma gondii
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    86664-plasmid-map-sequence-id-193761

    Enlarge

  21. hSLBP_mNG_FS_Donor

    Plasmid
    #191387
    Purpose
    Donor for C-terminal tagging of endogenous hSLBP
    Depositor
    Yannick Doyon
    Insert
    mNeonGreen(mNG) + FS(3xFLAG_TwinStrep)Tag for hSLBP tagging at the C-terminus (SLBP Synthetic, Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191387-plasmid-map-sequence-id-378113

    Enlarge

  23. pOTX2-donor

    Plasmid
    #176358
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    OTX2 (OTX2 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    176358-plasmid-map-sequence-id-347862

    Enlarge

  24. pSTAT5B-donor

    Plasmid
    #176339
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    STAT5B (STAT5B Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176339-plasmid-map-sequence-id-347884

    Enlarge

  25. pAW8-aid-eDSR-Turg1:kanMX6

    Plasmid
    #169362
    Purpose
    C-terminal aid-eDSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169362-plasmid-map-sequence-id-333207

    Enlarge

  26. pTUB:FLuc

    Plasmid
    #114578
    Purpose
    Constitutive expression of firefly luciferase in T. gondii using the alpha tubulin promoter; floxed pyrimethamine selectable marker; UPRT locus flanking sequence to direct integration
    Depositor
    David Sibley
    Insert
    Firefly Luciferase
    Use
    Cre/Lox and Luciferase
    Tags
    Expression
    Mutation
    Promoter
    alpha tubulin T. gondii
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    114578-plasmid-map-sequence-id-220131

    Enlarge

  27. pMDW133

    Plasmid
    #227248
    Purpose
    Lambda DNA for torsional templates
    Depositor
    Michelle Wang
    Insert
    Lambda DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Plac
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    227248-plasmid-map-sequence-id-450969

    Enlarge

  28. pXylP-HygR

    Plasmid
    #168131
    Purpose
    Expression of HygR from the xylose-inducible xylP promoter
    Depositor
    Michael Bromley
    Insert
    XylP-HygR
    Use
    Aspergillus expression
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    xylP
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    168131-plasmid-map-sequence-id-329723

    Enlarge

  29. pOSR1-donor

    Plasmid
    #176363
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    OSR1 (OSR1 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176363-plasmid-map-sequence-id-347404

    Enlarge

  30. pZNF627-donor

    Plasmid
    #176365
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ZNF627 (ZNF627 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    176365-plasmid-map-sequence-id-347863

    Enlarge

  31. pZNF608-donor

    Plasmid
    #176362
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ZNF608 (ZNF608 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    176362-plasmid-map-sequence-id-347402

    Enlarge

  32. pZNF79-donor

    Plasmid
    #176371
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ZNF79 (ZNF79 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    176371-plasmid-map-sequence-id-347399

    Enlarge

  33. pZBTB4-donor

    Plasmid
    #176374
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ZBTB4 (ZBTB4 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    176374-plasmid-map-sequence-id-347293

    Enlarge

  34. pSNAPC5-donor

    Plasmid
    #176341
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    SNAPC5 (SNAPC5 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    176341-plasmid-map-sequence-id-347185

    Enlarge

  35. pAW8-aid-spo5DSR-Turg1:kanMX6

    Plasmid
    #169358
    Purpose
    C-terminal aid-spo5DSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169358-plasmid-map-sequence-id-333930

    Enlarge

  36. pTSHZ1-donor

    Plasmid
    #132479
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    TSHZ1 (TSHZ1 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    none
    Promoter
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    132479-plasmid-map-sequence-id-274870

    Enlarge

  37. pZNF449-donor

    Plasmid
    #132515
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ZNF449 (ZNF449 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    none
    Promoter
    Available since
    March 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    132515-plasmid-map-sequence-id-275207

    Enlarge

  38. pZNF8-donor

    Plasmid
    #136689
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ZNF8 (ZNF8 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    136689-plasmid-map-sequence-id-274397

    Enlarge

  40. pMCMV3

    Plasmid
    #85711
    Purpose
    Expression vector employing the mouse CMV major immediate-early promoter enhancer (MIEP); there is a small polylinker (PstI, SmaI, XbaI, SalI ,HpaI) followed by the SV40 polyA signal sequences
    Depositor
    Ulrich Koszinowski, Martin Messerle
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MIEPmouse CMV MIEP
    Available since
    April 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    85711-plasmid-map-sequence-id-177907

    Enlarge

Showing: 1681 - 1720 of 4899 results