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pEBG-hBEN L38&45P

Plasmid

#22182

Depositor

Ananda Roy

Article

Tantin et al J Biol Chem. 2004 Feb 13. 279(7):5460-9.

Insert

hBEN (GTF2IRD1 Human)

Tags

GST and His

Expression

Mammalian

Mutation

Changed Leucine 38 to Proline and changed Leucine…

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pEBB-GFP-hBEN L38&45P

Plasmid

#22183

Depositor

Ananda Roy

Article

Tantin et al J Biol Chem. 2004 Feb 13. 279(7):5460-9.

Insert

hBEN (GTF2IRD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Luecine 38 to Proline and Leucine 45 to P…

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pWJPrP31

Plasmid

#22105

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

PrP exon 3 EcoR1 fragment

Use

Construction of targeting vector

Mutation

genomic EcoR1 fragment containing exon 3 of PrP

Available Since

Oct. 6, 2009

Availability

Academic Institutions and Nonprofits only
pWJPrP34

Plasmid

#22107

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

PrP exon 3 EcoR1 fragment

Use

Construction of targeting vector

Mutation

Added a Cla1 site right before translation start …

Available Since

Oct. 6, 2009

Availability

Academic Institutions and Nonprofits only
pWJPrP38

Plasmid

#22110

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

PrP genomic vector with cloning sites

Use

Construction of targeting vector

Mutation

2.2 Kb EcoR1 fragement isolated from pWJPrP34 and…

Available Since

Oct. 6, 2009

Availability

Academic Institutions and Nonprofits only
pEMB52 LRRK2_BC_1520_2210_I2211ENLYFQGWSHPQFEK

Plasmid

#40565

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and Tev-Strep (ENLYFQGWSHPQFEK)

Mutation

WT; aa1520_2210

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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AiP12214 - pAAV-ProB12-SYFP2-WPRE3-BGHpA (Alias: CN2214)

Plasmid

#208161

Purpose

AAV vector for SYFP2 expression in brain astrocytes

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

Beta Globin minimal promoter

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMDR1-221

Plasmid

#69481

Purpose

Deletion Construct

Depositor

Kathleen Scotto

Article

Jin et al Mol Cell Biol. 1998 Jul;18(7):4377-84.

Insert

MDR1 promoter (ABCB1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Insert includes MDR1 promoter sequence from -221 …

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
pCML 221

Plasmid

#98587

Purpose

pMS2-CYFP. Expresses MS2 protein-CYFP fusion in E. coli for TriFC assay (kanR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Insert

MS2

Promoter

pLacO

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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FN 221

Plasmid

#50498

Depositor

Kenneth Yamada

Insert

fibronectin (FN1 Human)

Tags

6X-His

Expression

Bacterial

Mutation

repeats 6-10, R1524K, D1526E, C1232A

Promoter

T7

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
AiP12211 - pAAV-AiE0050m_3xC2-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN2211)

Plasmid

#223974

Purpose

AiE0050m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L4/5_IT neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12218 - pAAV-AiE0058h_3xC2-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN2218)

Plasmid

#230358

Purpose

AiE0058h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minRho

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR221_EGFP

Plasmid

#25899

Purpose

Gateway entry vector containing EGFP.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

EGFP

Use

Gateway entry vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC20A1

Plasmid

#132010

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC20A1 (SLC20A1 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC12A3

Plasmid

#131920

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC12A3 (SLC12A3 Human)

Expression

Mammalian

Available Since

Oct. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pBA2219

Plasmid

#38152

Depositor

David Waugh

Article

Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

Insert

DsbC (dsbC E. coli)

Tags

N-terminal leader peptide (cloning artifact)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_SIK3_CR_T221E

Plasmid

#108108

Purpose

Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

change guanine 501 to cytosine (silent mutation),…

Promoter

EFS promoter

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC46A3

Plasmid

#132215

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC46A3 (SLC46A3 Human)

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC27A3

Plasmid

#132116

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC27A3 (SLC27A3 Human)

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC7A11

Plasmid

#132244

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC7A11 (SLC7A11 Human)

Expression

Mammalian

Available Since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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