We narrowed to 5,093 results for: puc

1. pUC19 gene 60 to gap + Cys

   Plasmid

   #49159

   Purpose

   Template for generating gene 60 mRNA, sequence 5' of coding gap + Cys

   Depositor

   Nils Walter

   Article

   Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

   Insert

   Bacteriophage T4 gene 600
   

   Mutation

   Coding gap and downstream sequence have been dele…

   Promoter

   T7

   Available Since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. puC19 preACT1(del6) A259C

   Plasmid

   #111329

   Purpose

   T7 transcription

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Expression

   Bacterial

   Mutation

   branch site A259C

   Availability

   Academic Institutions and Nonprofits only

   

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3. puC19 preACT1(del6) C256A

   Plasmid

   #111331

   Purpose

   T7 transcription

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Expression

   Bacterial

   Mutation

   branch site C256A

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19/PLIN2 GFP-P2A-BFP

   Plasmid

   #202195

   Purpose

   knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

   Depositor

   James Olzmann

   Article

   Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

   Insert

   PLIN2 (PLIN2 Human)
   

   Use

   Cloning vector

   Tags

   GFP-P2A-BFP knock-in

   Promoter

   lac

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC57-PB-PGK-tetR-KRAB-2A-Puro

   Plasmid

   #129536

   Purpose

   PiggyBac transposon carrying tetR-KRAB-2A-Puro under the PGK promoter.

   Depositor

   Hideyuki Okano

   Article

   Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

   Insert

   PB-PGK-tetR-KRAB-2A-Puro
   

   Use

   Unspecified

   Promoter

   hPGK promoter

   Available Since

   Dec. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-eGFP-P2A-dTAG-Ing3

   Plasmid

   #170754

   Purpose

   Donor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locus

   Depositor

   Kristian Helin

   Article

   Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

   Insert

   eGFP-P2A-2xHA-dTAG
   

   Use

   Mouse Targeting

   Tags

   eGFP-P2A-2xHA-dTAG

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   none

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIG-703pUC19-tNGFR-P2A-BACH2

   Plasmid

   #186112

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   BACH2 (BACH2 Human)
   

   Use

   CRISPR

   Mutation

   WT with tNGFR sequence

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIG-701pUC19-tNGFR-P2A-FOXP3

   Plasmid

   #186114

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   FOXP3 (FOXP3 Human)
   

   Use

   CRISPR

   Mutation

   WT with tNGFR sequence

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIG-704pUC19-tNGFR-P2A-STAT3

   Plasmid

   #186102

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   STAT3 (STAT3 Human)
   

   Use

   CRISPR

   Mutation

   WT with tNGFR sequence

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIG-705pUC19-tNGFR-P2A-STAT5b

    Plasmid

    #186103

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    STAT5B (STAT5B Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTR-177 pUC19-CLTA-N-mCherry

    Plasmid

    #186128

    Purpose

    knockin of mCherry to CLTA target

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CLTA (CLTA Human)
    

    Use

    CRISPR

    Mutation

    WT with mCherry sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC18T-mini-Tn7T-hph-Ptet2

    Plasmid

    #218368

    Purpose

    Empty backbone for Tn7-site insertion with tetR-PtetA expression system with two tetR operator sites

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)

    Plasmid

    #110559

    Purpose

    miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    rhaSR-PrhaBAD
    

    Expression

    Bacterial

    Promoter

    rhaSR-PrhaBAD

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-dual-scafffold-2.0 (pBP49)

    Plasmid

    #218931

    Purpose

    The dual-scaffold for making dual-CRISPR screening libraries or dual-CRISPR plasmids.

    Depositor

    Baoxu Pang

    Article

    Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

    Insert

    dual scaffolds for CRISPR
    

    Use

    CRISPR

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC18T-mini-Tn7T-Gm-Pc-DsRed-Express2

    Plasmid

    #111260

    Purpose

    mini-Tn7 vector for insertion of DsRed-Express2 at bacterial attTn7 site

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    DsRed-Express2
    

    Expression

    Bacterial

    Promoter

    Pc

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJM101(pUC18T-miniTn7T-gm-lacIq-Ptac)

    Plasmid

    #110558

    Purpose

    miniTn7 delivery plasmid with lacIq-Ptac inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    lacIq-Ptac
    

    Expression

    Bacterial

    Promoter

    lacIq-Ptac

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC118-mCtnnb1-FLIP-FlpE-Puro

    Plasmid

    #84541

    Purpose

    CRISPR - FLIP-FlpE targeting vector for mouse beta-catenin

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

    Insert

    mouse beta-catenin FLIP-FlpE - Puro targeting vector
    

    Use

    Cre/Lox and Mouse Targeting

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pUC19-TgKI-MCS-tdTomato-polyA

    Plasmid

    #174022

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    tdTomato
    

    Use

    Zebrafish knock-in tagging

    Available Since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19-P70a-Top-T~P70a-Bottom-T

    Plasmid

    #87314

    Purpose

    Expresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    P70a

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC18T-mini-Tn7T-Zeo-gfpmut3

    Plasmid

    #65037

    Purpose

    ZeoR mini-Tn7 vector, mobilizable, for GFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    gfpmut3
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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