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pHAGE-TO-dCas9-GFP

Plasmid

#64106

Purpose

Expression of Sp dCas9-GFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

sfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-SUN1_785

Plasmid

#125852

Purpose

Expresses SUN1_785 tagged with GFP in mammalian cells

Depositor

Miki Hieda

Article

Nishioka et al Nucleus. 2016 Nov;7(6):572-584. doi: 10.

Insert

SUN1_785 (SUN1 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nmdCas9-3XGFP

Plasmid

#64109

Purpose

Expression of Nm dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pLH-stsgRNA3.1

Plasmid

#64118

Purpose

Vector for expression of St1 sgRNA3.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA3.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pME-puro-SNAP-FLAG-CD59

Plasmid

#50374

Purpose

Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

SNAP

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE2-puro

Plasmid

#208032

Purpose

Enables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.

Depositor

Rosa Barrio, James Sutherland

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Type

Empty backbone

Expression

Insect

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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TALED_Right-ND1-AD

Plasmid

#183900

Purpose

Expresses TALED_Right-ND1-AD in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

TALED for ND1 mutation

Expression

Mammalian

Promoter

pCMV

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pUC57-PP7v3

Plasmid

#107230

Purpose

Expresses 24x optimized PP7 stem loops (version 3) in bacterial cells

Depositor

Jeffrey Chao

Article

Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

Insert

24xPP7v3

Tags

3x FLAG-tag and P2A site

Expression

Bacterial

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-mGFP-TAX1BP1

Plasmid

#192803

Purpose

Stable expression of mGFP-TAX1BP1 in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

monomeric EGFP (A206K)

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Cdc2-DN-HA

Plasmid

#1889

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdc2 (CDK1 Human)

Tags

HA

Expression

Mammalian

Mutation

D146N, dominant negative

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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mscv2.2-NAIP5

Plasmid

#60205

Purpose

Mouse NAIP5 (B6 allele), expressed under the constitutive, moderate-level retroviral LTR promoter for mammalian cell expression; IRES-GFP reporter downstream of NAIP5

Depositor

Russell Vance

Article

Tenthorey et al Mol Cell. 2014 Apr 10;54(1):17-29. doi:

Insert

NAIP5

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pTEI165

Plasmid

#126074

Purpose

Vector for expression of plastid targeted improved YFP1-10 (T65G,T205S, T203Y) in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

YFP1-10 (improved)

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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TALED_Left-ND1-E1347A

Plasmid

#183895

Purpose

Expresses TALED_Left-ND1-E1347A in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

TALED for ND1 mutation

Expression

Mammalian

Promoter

pCMV

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-PIK3R1

Plasmid

#174191

Purpose

Expresses PIK3R1 in mammalian cells

Depositor

Cheng-han Yu

Article

Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

Insert

PIK3R1 (PIK3R1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-INU-FLAG-FAM134Bs

Plasmid

#128261

Purpose

Expresses FAM134Bs in mammalian cells

Depositor

Noboru Mizushima

Article

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

FAM134Bs (RETREG1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pUb PspCas13b + PspCas13b guide RNA

Plasmid

#176305

Purpose

Expresses PspCas13b and its associated guide RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

Insert

PspCas13b

Tags

HA tag

Expression

Insect

Mutation

E113K, N265S- please depositor comments

Promoter

Ubi-p63e

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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YIplac211-NHX1-HaloTagx2

Plasmid

#177107

Purpose

Expresses Nhx1-HaloTagx2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

NHX1 (NHX1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Fra-2

Plasmid

#187907

Purpose

Expresses Fra-2 in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

Fra2 (Fosl2 Mouse)

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pX165-LZ3 Cas9

Plasmid

#140561

Purpose

Expresses LZ3 Cas9 in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

Cas9

Mutation

N690C T769I G915M N980K

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac211-NHX1-iGFP

Plasmid

#177108

Purpose

Expresses Nhx1-iGFP in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

NHX1 (NHX1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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