We narrowed to 15,965 results for: grna

1. pAAV ORANGE Dlg4-HaloTag KI

   Plasmid

   #139656

   Purpose

   Endogenous tagging of PSD95: C-terminal (amino acid position: R721)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and HaloTag donor
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGB 35s:dCas:BRD:tNos (GB1172)

   Plasmid

   #75401

   Purpose

   Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional Repressor

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   dCas9:BRD
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Promoter

   35S

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-PITCh-CLTC

   Plasmid

   #227312

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CLTC for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of CLTC (CLTC Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-itis pBE-t

   Plasmid

   #195342

   Purpose

   Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed gRNA targeting EGFP A110V

   Depositor

   Alexis Komor

   Article

   Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

   Inserts

   ecTadA(8e)-SpCas9-NG
   gRNA ctctacgcgggtcttgtagt
   

   Use

   CRISPR

   Mutation

   SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…

   Promoter

   Lac and ProC

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPK2101

   Plasmid

   #65770

   Purpose

   Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized SaCas9, and SaCas9 gRNA
   

   Use

   CRISPR

   Tags

   NLS-3xFlag

   Expression

   Bacterial

   Promoter

   T7 (x2)

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pORANGE Bsn-GFP KI

   Plasmid

   #139665

   Purpose

   Endogenous tagging of Bassoon: C-terminal (amino acid position: F3938)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-itis pBE-nt

   Plasmid

   #195343

   Purpose

   Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed nonsense non-targeting gRNA

   Depositor

   Alexis Komor

   Article

   Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

   Inserts

   ecTadA(8e)-SpCas9-NG
   gRNA gtgcacgacgccgtatgcga
   

   Use

   CRISPR

   Mutation

   SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…

   Promoter

   Lac and ProC

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV ORANGE Gria1-HaloTag KI

   Plasmid

   #139655

   Purpose

   Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and HaloTag donor
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-PITCh-PEX3

   Plasmid

   #227303

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PEX3 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of PEX3 (PEX3 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pORANGE GFP-Bsn KI

    Plasmid

    #139664

    Purpose

    Endogenous tagging of Bassoon: N-terminal (amino acid position: L7)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pORANGE GFP-Pclo KI

    Plasmid

    #139657

    Purpose

    Endogenous tagging of Piccolo: N-terminal (amino acid position: G2)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-guide-puro mMyh9 - 2

    Plasmid

    #198492

    Purpose

    lentiviral stable expression of mMyh9 gRNA 2

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mMyh9 (Myh9 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-guide-puro mMyh9 - 1

    Plasmid

    #198491

    Purpose

    lentiviral stable expression of mMyh9 gRNA 1

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mMyh9 (Myh9 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330.Pten.b

    Plasmid

    #66588

    Purpose

    sgRNA for Pten deletion

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    Pten deletion (Pten Mouse)
    

    Expression

    Mammalian

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

15. px330-GAPDH

    Plasmid

    #136940

    Purpose

    NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4

    Depositor

    Jeremy Stark

    Article

    Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

    Insert

    sgRNA targeting GAPDH (GAPDH Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. B52 + SMARCAL1 sgSTOP

    Plasmid

    #100715

    Purpose

    B52 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting SMARCAL1 (cloned using BbsI)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoters

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDIRECT_26H

    Plasmid

    #91150

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. B52 + SPRTN sgSTOP

    Plasmid

    #100709

    Purpose

    B52 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting SPRTN (cloned using BbsI)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoters

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330.Pten.a

    Plasmid

    #66587

    Purpose

    sgRNA for Pten deletion

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    Pten deletion (Pten Mouse)
    

    Expression

    Mammalian

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pMOD_C2616

    Plasmid

    #91090

    Purpose

    Module C, Promoter: At7SL, Gene: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Promoter

    At7SL

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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