We narrowed to 5,620 results for: Feng Zhang

1. pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D

   Plasmid

   #169912

   Purpose

   Expression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   dCjCas9
   

   Use

   CRISPR

   Tags

   10XGCN4, 2xSV40NLS, and SV40NLS-HA

   Expression

   Mammalian

   Mutation

   D8A, H559A in cjCas9

   Promoter

   CMV

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRGEN-CMV-VPR-L1-CjCas9 V-D

   Plasmid

   #169913

   Purpose

   Expression of a VPR-dCjCas9 fusion protein to activate gene expression.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   dCjCas9
   

   Use

   CRISPR

   Tags

   2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)

   Expression

   Mammalian

   Mutation

   D8A, H559A in cjCas9

   Promoter

   CMV

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRGEN-CMV-VPR-WT CjCas9 V-WT

   Plasmid

   #169914

   Purpose

   Expression of VPR-WT CjCas9 fusion protein to induce orthogonal genes knock out and activation.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   CjCas9
   

   Use

   CRISPR

   Tags

   2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Pdpy-30-miniCAFE

   Plasmid

   #170115

   Purpose

   Dpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   MiniCAFE
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D8A, HNH-truncation (Δ495–609 aa) in CjCas9

   Promoter

   Dpy-30

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hsyn-GRAB_5-HT1.0

   Plasmid

   #140552

   Purpose

   Expresses the 5-HT sensor GRAB_5-HT1.0 in neurons

   Depositor

   Yulong Li

   Article

   Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s

   Has Service

   AAV9

   Insert

   5-HT sensor GRAB_5-HT1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDisplay-GACh2.0

   Plasmid

   #106073

   Purpose

   express the genetically-encoded fluorescent ACh indicator GACh2.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

   Insert

   GPCR activation based ACh sensor (GACh) 2.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-GACh2.0

   Plasmid

   #121921

   Purpose

   express the genetically-encoded fluorescent acethycholine(ACh) sensor GACh2.0

   Depositor

   Yulong Li

   Article

   Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

   Insert

   GPCR activation based ACh sensor GACh2.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDisplay-GACh1.0

   Plasmid

   #106072

   Purpose

   express the genetically-encoded fluorescent ACh indicator GACh1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

   Insert

   GPCR activation based ACh sensor (GACh) 1.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.3-hALDOA

   Plasmid

   #210809

   Purpose

   For transient transfection

   Depositor

   Sheng-cai Lin

   Article

   Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

   Insert

   ALDOA (ALDOA Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Th-tdTomato

    Plasmid

    #159460

    Purpose

    Targeting vector to TH gene locus for induction of P2A-fused tdTomato, with floxed PGK-puroR drug resistant cassette

    Depositor

    Yuejun Chen

    Article

    Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

    Insert

    P2A-tdTomato
    

    Use

    Cre/Lox

    Tags

    P2A

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous TH

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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