We narrowed to 13,505 results for: Mpl

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WRN scFv [N428/12]

Plasmid

#206784

Purpose

Mammalian Expression of WRN scFV. Derived from hybridoma N428/12 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

WRN (Homo sapiens) recombinant scFV (WRN Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-mScarlet-ACTB

Plasmid

#207754

Purpose

Donor template for Puro-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

ACTB Homology Arms flanking a Puro-mScarlet Cassette (ACTB Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pMRX-IRES-puro-3xFLAG-NBR1 deltaUBA

Plasmid

#204547

Purpose

Expresses 3xFLAG-tagged NBR1 deltaUBA in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

NBR1 (NBR1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

deltaUBA

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pSICO-CPSF6-358-iRFP670

Plasmid

#110694

Purpose

Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with iRFP670

Depositor

Zandrea Ambrose

Article

Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18.

Insert

CPSF6-iRFP670 (CPSF6 Human)

Use

Lentiviral

Tags

iRFP670

Mutation

Truncation of CPSF6 isoform at amino acid 358

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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Parvalbumin scFv [L114/3]

Plasmid

#182072

Purpose

Mammalian Expression of Parvalbumin scFv. Derived from hybridoma L114/3.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Parvalbumin (Rattus norvegicus) (Pvalb Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {mCAR}off-{ChETA}on-WPRE

Plasmid

#111387

Purpose

AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

mCAR (Cxadr Mouse)
ChETA

Use

AAV

Tags

HA and Myc

Expression

Mammalian

Promoter

hSynapsin

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt

Plasmid

#157702

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hSyn-mCherry.3xFLAG.NOS1AP-S-WPRE

Plasmid

#127867

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP-S fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1ap (Nos1ap Mouse, Human)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

constructed by blunt end fusion of the 5’ 51 nucl…

Promoter

hSyn

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Pan-Neurofascin scFv [L11A/41]

Plasmid

#206722

Purpose

Mammalian Expression of Pan-Neurofascin scFV. Derived from hybridoma L11A/41 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Pan-Neurofascin (Rattus norvegicus) recombinant scFV (Nfasc Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Brg1 Del4 pET28C

Plasmid

#122118

Purpose

Expression Brg1 amino acids 1446-1647 in bacteria

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brg1 (SMARCA4 Human)

Tags

His-T7

Expression

Bacterial

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pLVCT-Dnd1-EGFP

Plasmid

#106343

Purpose

Lentiviral vector, mammalian expression of Dnd1-EGFP fusion protein

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1-EGFP (Dnd1 Mouse, Synthetic)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-hnRNPA2_deltaLC

Plasmid

#98664

Purpose

Expresses MBP-tagged hnRNPA2 without the LC domain

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

G190Stop

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1318-1605_W

Plasmid

#147893

Purpose

Mammalian Expression of HsNot1iso1_1318-1605

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1318-1605 (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-p35S-GFP(rc)13

Plasmid

#127526

Purpose

Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 13 attachment sites.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

EGFP reverse complement coding sequence flanked by attB/attP Integrase 13 attachment sites.

Expression

Plant

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-KIR3DX1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156774

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR3DX1 (KIR3DX1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-V1251R_R

Plasmid

#147376

Purpose

Mammalian Expression of HsNot1iso1-V1251R

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1-V1251R (CNOT1 Human)

Expression

Mammalian

Mutation

A deletion of 5 AA 822-827 SKMKPS-> T and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SIRPD-Fc(DAPA)-AviTag-6xHis

Plasmid

#156563

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIRPD (SIRPD Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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Brg1 Del1 pET28a

Plasmid

#122115

Purpose

Expression Brg1 amino acids 1-381 in bacteria

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brg1 (SMARCA4 Human)

Tags

His-T7

Expression

Bacterial

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-CTLA4-FLN-ELP-His-ybbr

Plasmid

#168038

Purpose

Expresses CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Cytotoxic T-lymphocyte associated protein 4 (CTLA4 Human)

Tags

3x ELP linker, 6x Histag, ddFLN4 (C18S), and ybbr…

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-HLA-G-COMP5AP-AviTag-9xHis

Plasmid

#157397

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

HLA-G (HLA-G Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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