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pT7-V5-SBP-C1-HsNanos2-del6-22_X

Plasmid

#147961

Purpose

Mammalian Expression of HsNanos2-del6-22

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos2-del6-22 (NANOS2 Human)

Expression

Mammalian

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP225-Lenti-CMV-Flag-GluN2A-WPRE-pA

Plasmid

#84274

Purpose

pLenti vector backbone designed to express Flag-GluN2A from a CMV promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Flag-GluN2A

Use

Lentiviral

Tags

Flag

Promoter

CMV

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP202-Lenti-TRE3G-GFPGluN2A-WPRE-pA

Plasmid

#164776

Purpose

pLenti vector backbone designed to express GFP-GluN2A from a TRE3G promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2A

Use

Lentiviral

Tags

GFP

Promoter

1.3αCaMKII

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP214-Lenti-0.5Synapsin P-GFP- WPRE-pA

Plasmid

#62929

Purpose

pLenti vector backbone designed to express GFP from a 0.5Synapsin promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

Lentiviral

Promoter

0.5synapsin and 1.3αCaMKII

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP215-Lenti-0.4CaMKIIa P-GFP-WPRE-pA

Plasmid

#62927

Purpose

pLenti vector backbone designed to express GFP from a 0.4CaMKIIa promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

Lentiviral

Promoter

0.4αCaMKII and 1.3αCaMKII

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-G-Cdc42

Plasmid

#180582

Purpose

ddGFP sensor of Cdc42

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Cdc42

Use

AAV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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RP51A-No5'ss-NoBS-NoPseudoBS

Plasmid

#100987

Purpose

RP51A with T7 promoter in HindIII/EcoRI sites of pUC18. No 5'ss, No BS, No pseudo BS

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

5'ss mut to C/ATACCT BS mut to GTTAGTG, pseu…

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-CAAAG

Plasmid

#170096

Purpose

deGFP reporter plasmid with CAAAG PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-CAATG

Plasmid

#170097

Purpose

deGFP reporter plasmid with CAATG PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-GTAAT

Plasmid

#170098

Purpose

deGFP reporter plasmid with GTAAT PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-GTATT

Plasmid

#170099

Purpose

deGFP reporter plasmid with GTATT PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEc-crRNA2

Plasmid

#170089

Purpose

encodes E. coli type I-E single spacer array

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E single spacer array

Use

CRISPR and Synthetic Biology

Promoter

J23119

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-ATAAC

Plasmid

#170095

Purpose

deGFP reporter plasmid with ATAAC PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSumo-His6- Sumo-OsTPL(1-209)

Plasmid

#177855

Purpose

Bacterial Expression of TPL

Depositor

Karsten Melcher

Article

Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

Insert

TPL

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pSumo-His6-SUMO-OsTPR2(1-210)

Plasmid

#177859

Purpose

Bacterial Expression of TPR2

Depositor

Karsten Melcher

Article

Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

Insert

TPR2

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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CDS_mTurqcp_chlo

Plasmid

#163936

Purpose

L0 part - CDS

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

FP optimised for Marchantia polymorpha chloroplast expression, from Genbank ID KT364744

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1-Ck2-smR-chlo

Plasmid

#163939

Purpose

L1 in position 2, for spectinomycin in chloroplast transformation

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

aadA gene for spectinomycin resistance

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCk1chlo

Plasmid

#163942

Purpose

Acceptor plasmid for odd level position 1

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCk2chlo

Plasmid

#163943

Purpose

Acceptor plasmid for odd level position 2

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCk3chlo

Plasmid

#163944

Purpose

Acceptor plasmid for odd level position 3

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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